SIGA_STRGG
ID SIGA_STRGG Reviewed; 514 AA.
AC B1VXR4;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=RNA polymerase principal sigma factor HrdB {ECO:0000305};
DE AltName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
GN Name=hrdB {ECO:0000303|PubMed:23347076};
GN Synonyms=sigA {ECO:0000255|HAMAP-Rule:MF_00963};
GN OrderedLocusNames=SGR_1701 {ECO:0000312|EMBL:BAG18530.1};
OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=455632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 4626 / NBRC 13350;
RX PubMed=18375553; DOI=10.1128/jb.00204-08;
RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H.,
RA Yamashita A., Hattori M., Horinouchi S.;
RT "Genome sequence of the streptomycin-producing microorganism Streptomyces
RT griseus IFO 13350.";
RL J. Bacteriol. 190:4050-4060(2008).
RN [2]
RP INDUCTION.
RC STRAIN=JCM 4626 / NBRC 13350;
RX PubMed=23347076; DOI=10.1111/mmi.12160;
RA Otani H., Higo A., Nanamiya H., Horinouchi S., Ohnishi Y.;
RT "An alternative sigma factor governs the principal sigma factor in
RT Streptomyces griseus.";
RL Mol. Microbiol. 87:1223-1236(2013).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- INDUCTION: Transcription is regulated by the alternative sigma factor
CC ShbA. {ECO:0000269|PubMed:23347076}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP009493; BAG18530.1; -; Genomic_DNA.
DR RefSeq; WP_003965769.1; NC_010572.1.
DR AlphaFoldDB; B1VXR4; -.
DR SMR; B1VXR4; -.
DR STRING; 455632.SGR_1701; -.
DR EnsemblBacteria; BAG18530; BAG18530; SGR_1701.
DR GeneID; 6210716; -.
DR KEGG; sgr:SGR_1701; -.
DR PATRIC; fig|455632.4.peg.1723; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_2_2_11; -.
DR OMA; QIPVTKW; -.
DR OrthoDB; 1565734at2; -.
DR Proteomes; UP000001685; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..514
FT /note="RNA polymerase principal sigma factor HrdB"
FT /id="PRO_0000442938"
FT DNA_BIND 475..494
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 135..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..351
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 360..436
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 449..502
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 305..308
FT /note="Interaction with polymerase core subunit RpoC"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT COMPBIAS 136..155
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..193
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 514 AA; 56133 MW; 77873D66597C8A8D CRC64;
MSASTSRTLP PEIAESESVM ALIERGKADG QIAGDDVRRA FEADQIPPTQ WKNVLRSLNQ
ILEEEGVTLM VSAAESPKRA RKSVAAKSPV KRTATKTVAA KTTVTRTVAA TAAPAVESAD
AADDAVAAAP AKKTAAKKAT AKKAAAKKTT AKKTAAKKSG KQDDEILDGD EAAEEVKAGK
GEEEEGEGEN KGFVLSDDDE DDAPAQQVAV AGATADPVKD YLKQIGKVPL LNAEQEVELA
KRIEAGLFAE DKLANADKLA PKLKRELEII AEDGRRAKNH LLEANLRLVV SLAKRYTGRG
MLFLDLIQEG NLGLIRAVEK FDYTKGYKFS TYATWWIRQA ITRAMADQAR TIRIPVHMVE
VINKLARVQR QMLQDLGREP TPEELAKELD MTPEKVIEVQ KYGREPISLH TPLGEDGDSE
FGDLIEDSEA VVPADAVSFT LLQEQLHSVL DTLSEREAGV VSMRFGLTDG QPKTLDEIGK
VYGVTRERIR QIESKTMSKL RHPSRSQVLR DYLD
