ID   SIGA_SYNY3              Reviewed;         425 AA.
AC   P74565; Q55351;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD, rpoD1;
GN   OrderedLocusNames=slr0653;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-396.
RC   STRAIN=559;
RA   Asayama M., Yamada A., Tanaka K., Takahashi H., Shirai M.;
RT   "A new set of PCR primers for specific detection of the gene encoding the
RT   principal sigma factor in cyanobacteria.";
RL   J. Gen. Appl. Microbiol. 42:511-515(1996).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; BA000022; BAA18672.1; -; Genomic_DNA.
DR   EMBL; D86213; BAA13043.1; -; Genomic_DNA.
DR   PIR; S76760; S76760.
DR   AlphaFoldDB; P74565; -.
DR   SMR; P74565; -.
DR   IntAct; P74565; 42.
DR   STRING; 1148.1653761; -.
DR   PaxDb; P74565; -.
DR   EnsemblBacteria; BAA18672; BAA18672; BAA18672.
DR   KEGG; syn:slr0653; -.
DR   eggNOG; COG0568; Bacteria.
DR   InParanoid; P74565; -.
DR   OMA; SMEMTIE; -.
DR   PhylomeDB; P74565; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..425
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093928"
FT   DNA_BIND        386..405
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          193..263
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          272..347
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          360..413
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           217..220
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   425 AA;  49936 MW;  4043FEA859B442D7 CRC64;
     MTQTKEPLTK AESAELEQEI ELSQYINTDD IDDDDIDVED LEQEVAATEG KEKKVRKIRK
     DAVKKKPYTE DSIRIYLQEI GRIRLLRAEE EIELARQIAD LLELELIRDN LTLQLERQPS
     ELEWGKQVWK LETAKQRLVG DKKKEPKKKD IDSYLANPDN ELSLENEWSQ QPNKNFAAFR
     RRLFLDRRAK DKMVQSNLRL VVSIAKKYMN RGLSFQDLIQ EGSLGLIRAA EKFDHEKGYK
     FSTYATWWIR QAITRAIADQ SRTIRLPVHL YETISRIKKT TKLLSQEMRR KPTEEEIAEK
     MEMTIEKLRF IAKSAQLPIS LETPIGKEED SRLGDFIEAD GETPEDEVSK NLLREDLENV
     LDTLSPRERD VLRLRYGLDD GRMKTLEEIG QIFNVTRERI RQIEAKALRK LRHPNRNSIL
     KEYIR