ID   SIGB_MYCTO              Reviewed;         323 AA.
AC   P9WGI4; O08496; O08514; Q59563; Q79FB5; Q7D6Q4;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=RNA polymerase sigma factor SigB;
GN   Name=sigB; Synonyms=mysB; OrderedLocusNames=MT2783;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. A non-essential principal sigma factor that responds to cell
CC       envelope stress and hypoxia (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer (Probable). Interacts transiently with the RNA
CC       polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha,
CC       1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase
CC       holoenzyme that can initiate transcription (By similarity).
CC       {ECO:0000250, ECO:0000305}.
CC   -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC       interaction with the -10 element in promoter DNA, and plays an
CC       important role in melting the double-stranded DNA and the formation of
CC       the transcription bubble. The sigma-70 factor domain-2 mediates
CC       interaction with the RNA polymerase subunits RpoB and RpoC (By
CC       similarity). {ECO:0000250}.
CC   -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC       H) motif that mediates interaction with the -35 element in promoter
CC       DNA. The domain also mediates interaction with the RNA polymerase
CC       subunit RpoA (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47099.1; -; Genomic_DNA.
DR   PIR; JC5011; JC5011.
DR   RefSeq; WP_003413958.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGI4; -.
DR   SMR; P9WGI4; -.
DR   EnsemblBacteria; AAK47099; AAK47099; MT2783.
DR   GeneID; 45426697; -.
DR   KEGG; mtc:MT2783; -.
DR   PATRIC; fig|83331.31.peg.2996; -.
DR   HOGENOM; CLU_014793_3_4_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..323
FT                   /note="RNA polymerase sigma factor SigB"
FT                   /id="PRO_0000428356"
FT   DNA_BIND        284..303
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..228
FT                   /note="Sufficient to interact with RbpA"
FT   REGION          25..59
FT                   /note="Sigma-70 factor domain-1"
FT   REGION          90..160
FT                   /note="Sigma-70 factor domain-2"
FT   REGION          169..245
FT                   /note="Sigma-70 factor domain-3"
FT   REGION          258..311
FT                   /note="Sigma-70 factor domain-4"
FT   MOTIF           114..117
FT                   /note="Polymerase core binding"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  36343 MW;  761DA93B9A18242F CRC64;
     MADAPTRATT SRVDSDLDAQ SPAADLVRVY LNGIGKTALL NAAGEVELAK RIEAGLYAEH
     LLETRKRLGE NRKRDLAAVV RDGEAARRHL LEANLRLVVS LAKRYTGRGM PLLDLIQEGN
     LGLIRAMEKF DYTKGFKFST YATWWIRQAI TRGMADQSRT IRLPVHLVEQ VNKLARIKRE
     MHQHLGREAT DEELAAESGI PIDKINDLLE HSRDPVSLDM PVGSEEEAPL GDFIEDAEAM
     SAENAVIAEL LHTDIRSVLA TLDEREHQVI RLRFGLDDGQ PRTLDQIGKL FGLSRERVRQ
     IERDVMSKLR HGERADRLRS YAS