SIGC_ARATH
ID SIGC_ARATH Reviewed; 571 AA.
AC O24621; O22058; Q8L7P7;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=RNA polymerase sigma factor sigC;
DE Short=Sigma factor C;
DE Short=Sigma-C;
DE AltName: Full=RNA polymerase sigma factor sig3;
DE Short=Atsig3;
DE Short=Sigma factor 3;
DE Flags: Precursor;
GN Name=SIGC; Synonyms=SIG3; OrderedLocusNames=At3g53920; ORFNames=F5K20.220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION BY LIGHT, AND GENE
RP FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=9280303; DOI=10.1016/s0014-5793(97)00906-x;
RA Tanaka K., Tozawa Y., Mochizuki N., Shinozaki K., Nagatani A., Wakasa K.,
RA Takahashi H.;
RT "Characterization of three cDNA species encoding plastid RNA polymerase
RT sigma factors in Arabidopsis thaliana: evidence for the sigma factor
RT heterogeneity in higher plant plastids.";
RL FEBS Lett. 413:309-313(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION BY LIGHT, AND GENE FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=9405719; DOI=10.1073/pnas.94.26.14948;
RA Isono K., Shimizu M., Yoshimoto K., Niwa Y., Satoh K., Yokota A.,
RA Kobayashi H.;
RT "Leaf-specifically expressed genes for polypeptides destined for
RT chloroplasts with domains of sigma70 factors of bacterial RNA polymerases
RT in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:14948-14953(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY LIGHT, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Seedling hypocotyl;
RX PubMed=10946105; DOI=10.1016/s0300-9084(00)00611-8;
RA Allison L.A.;
RT "The role of sigma factors in plastid transcription.";
RL Biochimie 82:537-548(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-571.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=10555304; DOI=10.1093/oxfordjournals.pcp.a029612;
RA Kanamaru K., Fujiwara M., Seki M., Katagiri T., Nakamura M., Mochizuki N.,
RA Nagatani A., Shinozaki K., Tanaka K., Takahashi H.;
RT "Plastidic RNA polymerase sigma factors in Arabidopsis.";
RL Plant Cell Physiol. 40:832-842(1999).
RN [9]
RP FUNCTION.
RX PubMed=10734058; DOI=10.1074/jbc.275.13.9215;
RA Hakimi M.-A., Privat I., Valay J.-G., Lerbs-Mache S.;
RT "Evolutionary conservation of C-terminal domains of primary sigma(70)-type
RT transcription factors between plants and bacteria.";
RL J. Biol. Chem. 275:9215-9221(2000).
RN [10]
RP INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=11959137; DOI=10.1016/s0014-5793(02)02538-3;
RA Tsunoyama Y., Morikawa K., Shiina T., Toyoshima Y.;
RT "Blue light specific and differential expression of a plastid sigma factor,
RT Sig5 in Arabidopsis thaliana.";
RL FEBS Lett. 516:225-228(2002).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=12602869; DOI=10.1023/a:1022095017355;
RA Privat I., Hakimi M.-A., Buhot L., Favory J.-J., Mache-Lerbs S.;
RT "Characterization of Arabidopsis plastid sigma-like transcription factors
RT SIG1, SIG2 and SIG3.";
RL Plant Mol. Biol. 51:385-399(2003).
RN [12]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=15807777; DOI=10.1111/j.1365-313x.2005.02362.x;
RA Ishizaki Y., Tsunoyama Y., Hatano K., Ando K., Kato K., Shinmyo A.,
RA Kobori M., Takeba G., Nakahira Y., Shiina T.;
RT "A nuclear-encoded sigma factor, Arabidopsis SIG6, recognizes sigma-70 type
RT chloroplast promoters and regulates early chloroplast development in
RT cotyledons.";
RL Plant J. 42:133-144(2005).
RN [13]
RP INDUCTION BY LIGHT.
RX PubMed=18532976; DOI=10.1111/j.1365-313x.2008.03567.x;
RA Onda Y., Yagi Y., Saito Y., Takenaka N., Toyoshima Y.;
RT "Light induction of Arabidopsis SIG1 and SIG5 transcripts in mature leaves:
RT differential roles of cryptochrome 1 and cryptochrome 2 and dual function
RT of SIG5 in the recognition of plastid promoters.";
RL Plant J. 55:968-978(2008).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of plastid-encoded RNA polymerase (PEP) to specific
CC initiation sites and are then released. {ECO:0000269|PubMed:10734058,
CC ECO:0000269|PubMed:12602869}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10555304, ECO:0000269|PubMed:12602869,
CC ECO:0000269|PubMed:9405719}. Note=Seems attached to membranes.
CC {ECO:0000269|PubMed:12602869}.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaves, to a lower extent in
CC cotyledons, and barely expressed in roots. Present in seeds, seedlings
CC and etiolated plantlets. {ECO:0000269|PubMed:10946105,
CC ECO:0000269|PubMed:12602869, ECO:0000269|PubMed:15807777,
CC ECO:0000269|PubMed:9405719}.
CC -!- INDUCTION: Slightly induced by blue light, especially after dark
CC adaptation. {ECO:0000269|PubMed:10946105, ECO:0000269|PubMed:11959137,
CC ECO:0000269|PubMed:18532976, ECO:0000269|PubMed:9280303,
CC ECO:0000269|PubMed:9405719}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM91534.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; D89994; BAA22530.1; -; mRNA.
DR EMBL; AB019944; BAA82450.1; -; Genomic_DNA.
DR EMBL; AB004822; BAA22215.1; -; mRNA.
DR EMBL; AF015544; AAB69386.1; -; mRNA.
DR EMBL; AL132960; CAB88354.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79159.1; -; Genomic_DNA.
DR EMBL; BT030362; ABO38775.1; -; mRNA.
DR EMBL; AY128331; AAM91534.1; ALT_INIT; mRNA.
DR PIR; T45932; T45932.
DR RefSeq; NP_190960.1; NM_115252.3.
DR AlphaFoldDB; O24621; -.
DR SMR; O24621; -.
DR STRING; 3702.AT3G53920.1; -.
DR iPTMnet; O24621; -.
DR PaxDb; O24621; -.
DR PRIDE; O24621; -.
DR EnsemblPlants; AT3G53920.1; AT3G53920.1; AT3G53920.
DR GeneID; 824559; -.
DR Gramene; AT3G53920.1; AT3G53920.1; AT3G53920.
DR KEGG; ath:AT3G53920; -.
DR Araport; AT3G53920; -.
DR TAIR; locus:2084420; AT3G53920.
DR eggNOG; ENOG502QSCF; Eukaryota.
DR HOGENOM; CLU_036956_0_0_1; -.
DR InParanoid; O24621; -.
DR OMA; ASMSCWA; -.
DR OrthoDB; 1191135at2759; -.
DR PhylomeDB; O24621; -.
DR PRO; PR:O24621; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O24621; baseline and differential.
DR Genevisible; O24621; AT.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IMP:TAIR.
DR GO; GO:0016987; F:sigma factor activity; IMP:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071482; P:cellular response to light stimulus; IEP:UniProtKB.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IDA:UniProtKB.
DR GO; GO:2001141; P:regulation of RNA biosynthetic process; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR016262; RNA_pol_sigma_SigB/C/D/F.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000767; RNA_pol_sigma_SigB/C/D; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; DNA-binding; Plastid; Reference proteome; Sigma factor;
KW Transcription; Transcription regulation; Transit peptide.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 33..571
FT /note="RNA polymerase sigma factor sigC"
FT /id="PRO_0000418095"
FT DNA_BIND 531..550
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 360..373
FT /note="Polymerase core binding"
FT CONFLICT 354
FT /note="L -> M (in Ref. 2; BAA22215)"
FT /evidence="ECO:0000305"
FT CONFLICT 538
FT /note="L -> F (in Ref. 2; BAA22215)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 571 AA; 64906 MW; 7F1A1651D2B4D1DF CRC64;
MASFNSFPIP KQIVGSSSSS SSSTSRPRIL VRSSLTSSMT STNSMLVFVH PHPLIKHWLS
LLRSDQTSFP IFVRIPMTSV VATRWSFLSS VKEESRIYQN DSLKACGCAS VSPYTAQNNV
YVELKDPKEN IGVGSAERSY SSRSMLQYNL LAKNLLALEE TFVALDSVRM ERDIMLQMGK
LGAAELFKTC LSRYRGSSIT SCLSDTTELV DTTPNQQVFV SSRRKVKKKA RRSSVTAENG
DQSSLPIGLR TTWNNIDVPR VRRPPKYRKK RERISRNETE MSTGVKIVAD MERIRTQLEE
ESGKVASLSC WAAAAGMNEK LLMRNLHYGW YCRDELVKST RSLVLFLARN YRGLGIAHED
LIQAGYVGVL QGAERFDHTR GYKFSTYVQY WIRKSMSTMV SRHARGVHIP SSIIRTINHI
QKARKTLKTS HGIKYAADEE IAKLTGHSVK KIRAANQCLK VVGSIDKKVG DCFTTKFLEF
TPDTTMESPE EAVMRQSARR DIHDLLEGLE PREKQVMVLR YGLQDYRPKS LEEIGKLLKV
SKEWIRKIER RAMAKLRDQP NAEDLRYYLN Q
