SIGE_MYCS2
ID SIGE_MYCS2 Reviewed; 257 AA.
AC A0R2D4; I7GEQ0; O05767;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=ECF RNA polymerase sigma factor SigE;
DE Short=ECF sigma factor SigE;
DE AltName: Full=Alternative RNA polymerase sigma factor SigE;
DE AltName: Full=RNA polymerase sigma-E factor;
DE Short=Sigma-E factor;
GN Name=sigE; OrderedLocusNames=MSMEG_5072, MSMEI_4945;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=9139909; DOI=10.1128/jb.179.9.2922-2929.1997;
RA Wu Q.L., Kong D., Lam K., Husson R.N.;
RT "A mycobacterial extracytoplasmic function sigma factor involved in
RT survival following stress.";
RL J. Bacteriol. 179:2922-2929(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP INTERACTION WITH RSEA.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=20025669; DOI=10.1111/j.1365-2958.2009.07008.x;
RA Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.;
RT "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis,
RT is inactivated by phosphorylation-dependent ClpC1P2 proteolysis.";
RL Mol. Microbiol. 75:592-606(2010).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Extracytoplasmic function (ECF) sigma factors are held in an
CC inactive form by an anti-sigma factor until released (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC transcription. Interacts (via sigma-70 factor domain 4) with RseA;
CC interaction is abrogated by treatment of cells with H(2)O(2) or
CC detergent. {ECO:0000269|PubMed:20025669}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-
CC sigma factors prevents interaction of sigma factors with the RNA
CC polymerase catalytic core (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Reduced survival after heat shock (50 degrees
CC Celsius), treatment at pH 4.0, exposure to detergent and oxidative
CC stress (5 mM H(2)O(2)). Additionally an inducible protective response
CC to oxidative stress present in the wild type was absent in the mutant.
CC {ECO:0000269|PubMed:9139909}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC45221.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AFP41389.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U87307; AAC45221.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP000480; ABK70433.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP41389.1; ALT_INIT; Genomic_DNA.
DR RefSeq; YP_889322.1; NC_008596.1.
DR AlphaFoldDB; A0R2D4; -.
DR SMR; A0R2D4; -.
DR STRING; 246196.MSMEI_4945; -.
DR EnsemblBacteria; ABK70433; ABK70433; MSMEG_5072.
DR EnsemblBacteria; AFP41389; AFP41389; MSMEI_4945.
DR KEGG; msg:MSMEI_4945; -.
DR KEGG; msm:MSMEG_5072; -.
DR PATRIC; fig|246196.19.peg.4950; -.
DR eggNOG; COG1595; Bacteria.
DR OMA; RIAINQC; -.
DR OrthoDB; 1914729at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009628; P:response to abiotic stimulus; IEA:UniProt.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..257
FT /note="ECF RNA polymerase sigma factor SigE"
FT /id="PRO_0000422946"
FT DNA_BIND 211..230
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 87..153
FT /note="Sigma-70 factor domain-2"
FT REGION 186..236
FT /note="Sigma-70 factor domain-4"
FT MOTIF 111..114
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255"
FT CONFLICT 41
FT /note="S -> P (in Ref. 1; AAC45221)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 257 AA; 29005 MW; 41049C27E5EA6A7E CRC64;
MEHDDRRASQ VRAGNNRVVI RVEQIENNAV HEQEGSTTIA SQPVTATHAA PVSMAHLEQF
TDSDWVEPSD EPTGTAVFDA TGDQAAMPSW DELVRQHADR VYRLAYRLSG NQHDAEDLTQ
ETFIRVFRSV QNYQPGTFEG WLHRITTNLF LDMVRRRGRI RMEALPEDYD RVPAEDPNPE
QIYHDSRLGA DLQAALDSLP PEFRAAVVLC DIEGLSYEEI GATLGVKLGT VRSRIHRGRQ
QLRDYLAKHS SETAQSA
