ABF2_CAEEL
ID ABF2_CAEEL Reviewed; 85 AA.
AC G5EC68; Q9NL69;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Antibacterial factor-related peptide 2 {ECO:0000303|PubMed:11772394};
DE Flags: Precursor;
GN Name=abf-2 {ECO:0000312|WormBase:C50F2.10};
GN ORFNames=C50F2.10 {ECO:0000312|WormBase:C50F2.10};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:BAA89490.1};
RN [1] {ECO:0000312|EMBL:BAA89490.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, MASS SPECTROMETRY, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=Bristol N2 {ECO:0000303|PubMed:11772394};
RX PubMed=11772394; DOI=10.1042/0264-6021:3610221;
RA Kato Y., Aizawa T., Hoshino H., Kawano K., Zhang H.;
RT "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis
RT elegans.";
RL Biochem. J. 361:221-230(2002).
RN [2] {ECO:0000312|EMBL:CCD67778.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CCD67778.1};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Exhibits antimicrobial activity against the Gram-positive
CC bacteria B.subtilis IFO 3134, K.varians MAFF 118076 and S.aureus ATCC
CC 6538P, the Gram-negative bacteria A.tumefaciens MAFF 1001,
CC B.bacteriovorus MAFF 106101 and K.pneumoniae MAFF 519002, and the
CC yeasts C.krusei MAFF 114085, K.thermotolerans MAFF 113848 and
CC T.delbrueckii MAFF 113811. {ECO:0000269|PubMed:11772394}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000303|PubMed:11772394}.
CC -!- TISSUE SPECIFICITY: Expressed in the pharynx (at protein level).
CC Detected in pharyngeal neurons and secretory cells.
CC {ECO:0000269|PubMed:11772394}.
CC -!- DEVELOPMENTAL STAGE: Expressed in all larval and adult stages but not
CC in pre-hatching embryos (at protein level).
CC {ECO:0000269|PubMed:11772394}.
CC -!- MASS SPECTROMETRY: Mass=6537.3; Method=Unknown;
CC Evidence={ECO:0000269|PubMed:11772394};
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DR EMBL; AB029810; BAA89490.1; -; mRNA.
DR EMBL; AB029811; BAA89492.1; -; mRNA.
DR EMBL; FO080915; CCD67778.1; -; Genomic_DNA.
DR RefSeq; NP_491252.1; NM_058851.4.
DR PDB; 5IX5; NMR; -; A=19-85.
DR PDBsum; 5IX5; -.
DR AlphaFoldDB; G5EC68; -.
DR SMR; G5EC68; -.
DR STRING; 6239.C50F2.10; -.
DR PaxDb; G5EC68; -.
DR PeptideAtlas; G5EC68; -.
DR EnsemblMetazoa; C50F2.10.1; C50F2.10.1; WBGene00000013.
DR GeneID; 266826; -.
DR KEGG; cel:CELE_C50F2.10; -.
DR UCSC; C50F2.10; c. elegans.
DR CTD; 266826; -.
DR WormBase; C50F2.10; CE27876; WBGene00000013; abf-2.
DR eggNOG; ENOG502TIHM; Eukaryota.
DR GeneTree; ENSGT00970000196640; -.
DR HOGENOM; CLU_172752_1_0_1; -.
DR InParanoid; G5EC68; -.
DR OMA; PHKSTCA; -.
DR OrthoDB; 1813671at2759; -.
DR PhylomeDB; G5EC68; -.
DR PRO; PR:G5EC68; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000013; Expressed in adult organism and 1 other tissue.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IEP:WormBase.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IEP:WormBase.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.110; -; 1.
DR InterPro; IPR031770; Abf-1/2.
DR InterPro; IPR038204; Abf-1/2_sf.
DR PANTHER; PTHR37971; PTHR37971; 1.
DR Pfam; PF16839; Antimicrobial25; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW Fungicide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000269|PubMed:11772394"
FT PEPTIDE 18..81
FT /note="Antibacterial factor-related peptide 2"
FT /evidence="ECO:0000269|PubMed:11772394"
FT /id="PRO_0000430527"
FT PROPEP 82..85
FT /evidence="ECO:0000303|PubMed:11772394"
FT /id="PRO_0000430528"
FT HELIX 31..33
FT /evidence="ECO:0007829|PDB:5IX5"
FT HELIX 34..46
FT /evidence="ECO:0007829|PDB:5IX5"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:5IX5"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:5IX5"
FT STRAND 61..64
FT /evidence="ECO:0007829|PDB:5IX5"
FT STRAND 70..74
FT /evidence="ECO:0007829|PDB:5IX5"
FT TURN 78..81
FT /evidence="ECO:0007829|PDB:5IX5"
SQ SEQUENCE 85 AA; 8931 MW; 2415DCF1863BB77C CRC64;
MFVRSLFLAL LLATIVAADI DFSTCARMDV PILKKAAQGL CITSCSMQNC GTGSCKKRSG
RPTCVCYRCA NGGGDIPLGA LIKRG
