SIGF_MYCTO
ID SIGF_MYCTO Reviewed; 261 AA.
AC P9WGI2; F2GKV9; Q50547; Q798K1; Q7D5S2;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=RNA polymerase sigma factor SigF;
DE Short=Sigma factor SigF;
DE AltName: Full=Alternative RNA polymerase sigma factor SigF;
DE AltName: Full=RNA polymerase sigma-F factor;
DE Short=Sigma-F factor;
DE AltName: Full=Stress response/stationary phase sigma factor SigF;
GN Name=sigF; OrderedLocusNames=MT3385;
OS Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83331;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT laboratory strains.";
RL J. Bacteriol. 184:5479-5490(2002).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=10992456; DOI=10.1128/iai.68.10.5575-5580.2000;
RA Chen P., Ruiz R.E., Li Q., Silver R.F., Bishai W.R.;
RT "Construction and characterization of a Mycobacterium tuberculosis mutant
RT lacking the alternate sigma factor gene, sigF.";
RL Infect. Immun. 68:5575-5580(2000).
RN [3]
RP FUNCTION, REGULON, AND DISRUPTION PHENOTYPE.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=14977982; DOI=10.1128/iai.72.3.1733-1745.2004;
RA Geiman D.E., Kaushal D., Ko C., Tyagi S., Manabe Y.C., Schroeder B.G.,
RA Fleischmann R.D., Morrison N.E., Converse P.J., Chen P., Bishai W.R.;
RT "Attenuation of late-stage disease in mice infected by the Mycobacterium
RT tuberculosis mutant lacking the SigF alternate sigma factor and
RT identification of SigF-dependent genes by microarray analysis.";
RL Infect. Immun. 72:1733-1745(2004).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Held in an inactive form by a cognate anti-sigma factor RsbW
CC (UsfX) until released. Its regulon, determined following disruption,
CC consists of 38 genes decreased in exponential phase, 187 genes
CC decreased in early S-phase, and 277 genes decreased in late S-phase
CC (PubMed:14977982). {ECO:0000269|PubMed:14977982}.
CC -!- SUBUNIT: Monomer. Interacts transiently with the RNA polymerase
CC catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1
CC beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that
CC can initiate transcription. Interacts (via sigma-70 factor domain 4)
CC with anti-sigma-F factor RsbW (UsfX) (By similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-
CC sigma factors prevents interaction of sigma factors with the RNA
CC polymerase catalytic core (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Not essential; grows to a higher density in
CC stationary phase and a slight increase in susceptibility to rifamycin
CC antibiotics. No effect on short-term intracellular survival and
CC proliferation in human monocytes in vitro. BALB/c mice infected with
CC mutant bacteria survived significantly longer (median survival 264 days
CC versus 161 days for wild-type) and lost significantly less weight
CC (PubMed:10992456). The CDC 1551 disruption in BALB/c and outbred Swiss-
CC Webster mice showed smaller and fewer lesions and less inflammation in
CC the lungs and spleen, and gave rabbits some degree of protection
CC against subsequent M.bovis infection (PubMed:14977982).
CC {ECO:0000269|PubMed:10992456, ECO:0000269|PubMed:14977982}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; AE000516; AAK47728.1; -; Genomic_DNA.
DR RefSeq; WP_003417158.1; NZ_KK341227.1.
DR AlphaFoldDB; P9WGI2; -.
DR SMR; P9WGI2; -.
DR EnsemblBacteria; AAK47728; AAK47728; MT3385.
DR GeneID; 45427282; -.
DR KEGG; mtc:MT3385; -.
DR PATRIC; fig|83331.31.peg.3643; -.
DR HOGENOM; CLU_014793_8_5_11; -.
DR Proteomes; UP000001020; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014322; RNA_pol_sigma-B/F/G.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02980; SigBFG; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE 3: Inferred from homology;
KW DNA-binding; Sigma factor; Transcription; Transcription regulation.
FT CHAIN 1..261
FT /note="RNA polymerase sigma factor SigF"
FT /id="PRO_0000428357"
FT DNA_BIND 231..250
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 43..112
FT /note="Sigma-70 factor domain-2"
FT REGION 123..189
FT /note="Sigma-70 factor domain-3"
FT REGION 209..258
FT /note="Sigma-70 factor domain-4"
FT MOTIF 67..70
FT /note="Interaction with polymerase core subunit RpoC"
FT /evidence="ECO:0000250"
SQ SEQUENCE 261 AA; 28794 MW; 7FD4AA6AB64061AB CRC64;
MTARAAGGSA SRANEYADVP EMFRELVGLP AGSPEFQRHR DKIVQRCLPL ADHIARRFEG
RGEPRDDLIQ VARVGLVNAA VRFDVKTGSD FVSFAVPTIM GEVRRHFRDN SWSVKVPRRL
KELHLRLGTA TADLSQRLGR APSASELAAE LGMDRAEVIE GLLAGSSYHT LSIDSGGGSD
DDARAITDTL GDVDAGLDQI ENREVLRPLL EALPERERTV LVLRFFDSMT QTQIAERVGI
SQMHVSRLLA KSLARLRDQL E
