SIGH_MYCS2
ID SIGH_MYCS2 Reviewed; 215 AA.
AC A0QTP2; I7G5A1; Q9XCD8;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=ECF RNA polymerase sigma factor SigH;
DE Short=ECF sigma factor SigH;
DE AltName: Full=Alternative RNA polymerase sigma factor SigH;
DE AltName: Full=RNA polymerase sigma-H factor;
DE Short=Sigma-H factor;
GN Name=sigH; Synonyms=rpoE; OrderedLocusNames=MSMEG_1914, MSMEI_1874;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=10400584; DOI=10.1128/jb.181.14.4266-4274.1999;
RA Fernandes N.D., Wu Q.-L., Kong D., Puyang X., Garg S., Husson R.N.;
RT "A mycobacterial extracytoplasmic sigma factor involved in survival
RT following heat shock and oxidative stress.";
RL J. Bacteriol. 181:4266-4274(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP FUNCTION, PROBABLE INTERACTION WITH RSHA, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=14617153; DOI=10.1046/j.1365-2958.2003.03739.x;
RA Song T., Dove S.L., Lee K.H., Husson R.N.;
RT "RshA, an anti-sigma factor that regulates the activity of the
RT mycobacterial stress response sigma factor SigH.";
RL Mol. Microbiol. 50:949-959(2003).
RN [6]
RP INDUCTION, AND PROBABLE ALTERNATIVE INITIATION (SIGH PRIME).
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=19682253; DOI=10.1111/j.1365-2958.2009.06824.x;
RA Kim M.S., Hahn M.Y., Cho Y., Cho S.N., Roe J.H.;
RT "Positive and negative feedback regulatory loops of thiol-oxidative stress
RT response mediated by an unstable isoform of sigmaR in actinomycetes.";
RL Mol. Microbiol. 73:815-825(2009).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Extracytoplasmic function (ECF) sigma factors are held in an
CC inactive form by an anti-sigma factor until released. This sigma factor
CC is involved in heat shock and oxidative stress responses.
CC {ECO:0000269|PubMed:14617153}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC transcription. Interacts (via sigma-70 factor domain-4) with anti-
CC sigma-H factor RshA, probably under reducing conditions, which prevents
CC its interaction with RNA polymerase.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=SigH;
CC IsoId=A0QTP2-1; Sequence=Displayed;
CC Name=SigH prime;
CC IsoId=A0QTP2-2; Sequence=VSP_053230;
CC -!- INDUCTION: Expressed at low levels at 37 degrees Celsius; 5-fold
CC further induced after heat shock (50 degress Celsius). A longer form is
CC transiently induced by diamide (at protein level). Autoregulated. Part
CC of the sigH-rshA operon. {ECO:0000269|PubMed:10400584,
CC ECO:0000269|PubMed:19682253}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-
CC sigma factors prevents interaction of sigma factors with the RNA
CC polymerase catalytic core (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Increased sensitivity to cumene hydroperoxide but
CC not H(2)O(2) (PubMed:10400584). Increased sensitivity to the oxidant
CC diamide (PubMed:14617153). {ECO:0000269|PubMed:10400584,
CC ECO:0000269|PubMed:14617153}.
CC -!- MISCELLANEOUS: [Isoform SigH]: Constitutively expressed major isoform,
CC not induced by diamide, after 60 minutes diamide no longer present (at
CC protein level).
CC -!- MISCELLANEOUS: [Isoform SigH prime]: Transiently produced upon diamide
CC induction. The longer form is seen in gels but the actual sequence is
CC unproven. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK71813.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF144091; AAD41810.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK71813.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001663; AFP38346.1; -; Genomic_DNA.
DR RefSeq; WP_003893296.1; NC_008596.1.
DR RefSeq; YP_886280.1; NC_008596.1.
DR AlphaFoldDB; A0QTP2; -.
DR SMR; A0QTP2; -.
DR STRING; 246196.MSMEI_1874; -.
DR EnsemblBacteria; ABK71813; ABK71813; MSMEG_1914.
DR EnsemblBacteria; AFP38346; AFP38346; MSMEI_1874.
DR GeneID; 66733349; -.
DR KEGG; msg:MSMEI_1874; -.
DR KEGG; msm:MSMEG_1914; -.
DR PATRIC; fig|246196.19.peg.1896; -.
DR eggNOG; COG1595; Bacteria.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009628; P:response to abiotic stimulus; IEA:UniProt.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014293; RNA_pol_sigma70_actinobac.
DR InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02947; SigH_actino; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS01063; SIGMA70_ECF; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; DNA-binding; Reference proteome; Sigma factor;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..215
FT /note="ECF RNA polymerase sigma factor SigH"
FT /id="PRO_0000423645"
FT DNA_BIND 163..182
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..96
FT /note="Sigma-70 factor domain-2"
FT REGION 137..188
FT /note="Sigma-70 factor domain-4"
FT MOTIF 53..56
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1
FT /note="M -> MVPTVCLERSALAGWDRGQLGGAVIEGKVFPTM (in isoform
FT SigH prime)"
FT /evidence="ECO:0000305"
FT /id="VSP_053230"
SQ SEQUENCE 215 AA; 24500 MW; 1A933B868A8CA757 CRC64;
MTDVDRVEPE TPPEREETDA ELTARFERDA IPLLDQLYGG ALRMTRNPAD AEDLLQETMV
KAYAGFRSFR EGTNLKAWLY RILTNTYINS YRKKQRQPSE YPTDEITDWQ LASNAEHSST
GLRSAEVEAL EALPDTEIKA ALQALPEEFR MAVYYADVEG FPYKEIAEIM ETPIGTVMSR
LHRGRRQLRD LLAGVARDRG FIRGPQLGEP EEVTS
