SIGI1_ACET2
ID SIGI1_ACET2 Reviewed; 256 AA.
AC A3DBH0;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=RNA polymerase sigma factor SigI1 {ECO:0000305};
GN Name=sigI1 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_0058 {ECO:0000312|EMBL:ABN51299.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP FUNCTION, ACTIVITY REGULATION, INTERACTION WITH RSGI1, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism.
CC SigI1 promotes transcription from sigI1 and celS promoters
CC (PubMed:20937888). {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI1 (PubMed:20937888). Binding of the polysaccharide substrate to
CC RsgI1 may lead to the release and activation of SigI1 (Probable).
CC {ECO:0000269|PubMed:20937888, ECO:0000305|PubMed:20937888}.
CC -!- SUBUNIT: Interacts with RsgI1. {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:20937888}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Up-regulated in the presence of cellulose.
CC {ECO:0000269|PubMed:20937888}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN51299.1; -; Genomic_DNA.
DR RefSeq; WP_011837745.1; NC_009012.1.
DR PDB; 6IVU; NMR; -; B=140-249.
DR PDBsum; 6IVU; -.
DR AlphaFoldDB; A3DBH0; -.
DR BMRB; A3DBH0; -.
DR SMR; A3DBH0; -.
DR DIP; DIP-59449N; -.
DR IntAct; A3DBH0; 1.
DR STRING; 203119.Cthe_0058; -.
DR EnsemblBacteria; ABN51299; ABN51299; Cthe_0058.
DR KEGG; cth:Cthe_0058; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; AFESFAH; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IDA:CACAO.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Reference proteome; Sigma factor;
KW Transcription; Transcription regulation.
FT CHAIN 1..256
FT /note="RNA polymerase sigma factor SigI1"
FT /id="PRO_0000436515"
FT DNA_BIND 205..224
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 67..80
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT HELIX 146..159
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 163..168
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 174..189
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 191..200
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 205..212
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 216..221
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 223..234
FT /evidence="ECO:0007829|PDB:6IVU"
FT HELIX 238..248
FT /evidence="ECO:0007829|PDB:6IVU"
SQ SEQUENCE 256 AA; 30580 MW; 348925F09F11E090 CRC64;
MEVRKINTHN REKKLDDIVY DDFISTLRQI KEGNHQLREE FISEYKPFIL KVTSNATGKY
IDTRNSDEFS IALSAFNEAI DKFDIEKGYN FFLFSEQVIR RRLIDYSRSN KDDKEYPFSF
FDDEYFYNNE KLLSKSYIGF EDIEAREDIE ELKKKLQEFG ITFLDLVLNV PKHRDSRQLC
IRLAKMLAED EQMYNALMKN KNIPRNELKK KAKVHGRTIG NNRKYIIALC LIFRSNLNLS
KRYLEYYTMD GESDLI
