SIGI2_ACET2
ID SIGI2_ACET2 Reviewed; 265 AA.
AC A3DC28;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=RNA polymerase sigma factor SigI2 {ECO:0000305};
GN Name=sigI2 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_0268 {ECO:0000312|EMBL:ABN51507.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP FUNCTION, INTERACTION WITH RSGI2, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism
CC (Probable). {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000305|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI2 (By similarity). Binding of the polysaccharide substrate to RsgI2
CC may lead to the release and activation of SigI2 (By similarity).
CC {ECO:0000250|UniProtKB:A3DBH0, ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI2. {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:20937888}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Up-regulated in the presence of cellulose.
CC {ECO:0000269|PubMed:20937888}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN51507.1; -; Genomic_DNA.
DR RefSeq; WP_003512418.1; NC_009012.1.
DR AlphaFoldDB; A3DC28; -.
DR SMR; A3DC28; -.
DR DIP; DIP-59451N; -.
DR IntAct; A3DC28; 1.
DR STRING; 203119.Cthe_0268; -.
DR EnsemblBacteria; ABN51507; ABN51507; Cthe_0268.
DR KEGG; cth:Cthe_0268; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; RYLTWGN; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..265
FT /note="RNA polymerase sigma factor SigI2"
FT /id="PRO_0000436516"
FT DNA_BIND 211..230
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 71..84
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 265 AA; 30744 MW; 2E5C873D33395AC9 CRC64;
MIDLFSPKGK KDTVSTTNKD KSFEDGIVNI INKIKAGDKL LREEFINSYT PYIIRTVSNL
TGKYVDVENS DEFSVGLAAF NEAIDSFDEG KNMFFFKFST LVIKRRLTDY ARHNKKHCHV
YPFTYFEDKN NSYFEQIHLK SEIDLQNTYE ISREIELYEQ KLRDFGISLE HLAKCAPKHK
DSKSLCIKIA KVIADNKELF SKLERTRNIP KTELLKLLKI NKKTIERNRT FIIAAALIFG
NDFNLLKDFL DISEPGGDNI ERSTK
