SIGI3_ACET2
ID SIGI3_ACET2 Reviewed; 249 AA.
AC A3DC74;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=RNA polymerase sigma factor SigI3 {ECO:0000305};
GN Name=sigI3 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_0315 {ECO:0000312|EMBL:ABN51553.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Borovok I.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
RN [5]
RP INDUCTION.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=24782837; DOI=10.3389/fmicb.2014.00142;
RA Wei H., Fu Y., Magnusson L., Baker J.O., Maness P.C., Xu Q., Yang S.,
RA Bowersox A., Bogorad I., Wang W., Tucker M.P., Himmel M.E., Ding S.Y.;
RT "Comparison of transcriptional profiles of Clostridium thermocellum grown
RT on cellobiose and pretreated yellow poplar using RNA-Seq.";
RL Front. Microbiol. 5:142-142(2014).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=26731480; DOI=10.1371/journal.pone.0146316;
RA Munoz-Gutierrez I., Ortiz de Ora L., Rozman Grinberg I., Garty Y.,
RA Bayer E.A., Shoham Y., Lamed R., Borovok I.;
RT "Decoding biomass-sensing regulons of Clostridium thermocellum alternative
RT sigma-I factors in a heterologous Bacillus subtilis host system.";
RL PLoS ONE 11:E0146316-E0146316(2016).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism
CC (Probable). Recognizes the predicted promoters associated with sigI3
CC itself, pl11, ce12 and cipA (PubMed:26731480). {ECO:0000255|HAMAP-
CC Rule:MF_02064, ECO:0000269|PubMed:26731480,
CC ECO:0000305|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI3 (By similarity). Binding of the polysaccharide substrate to RsgI3
CC may lead to the release and activation of SigI3 (By similarity).
CC {ECO:0000250|UniProtKB:A3DBH0, ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI3. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Autoregulated (Probable). Up-regulated in the presence of
CC xylan (PubMed:20937888). Up-regulated in pretreated yellow poplar
CC (PYP)-grown cells (PubMed:24782837). {ECO:0000269|PubMed:20937888,
CC ECO:0000269|PubMed:24782837, ECO:0000305|PubMed:26731480}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000568; ABN51553.1; -; Genomic_DNA.
DR EMBL; KM504391; AIY67764.1; -; Genomic_DNA.
DR RefSeq; WP_003512514.1; NC_009012.1.
DR AlphaFoldDB; A3DC74; -.
DR SMR; A3DC74; -.
DR STRING; 203119.Cthe_0315; -.
DR EnsemblBacteria; ABN51553; ABN51553; Cthe_0315.
DR KEGG; cth:Cthe_0315; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; KYVSCEN; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..249
FT /note="RNA polymerase sigma factor SigI3"
FT /id="PRO_0000436517"
FT DNA_BIND 199..218
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 60..73
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 249 AA; 29048 MW; 6564714540DD3496 CRC64;
MHGLFVNKKK NDTGSTALVL KKIQSGDTKL KEEFIKDNVP YIIRTISNIL GIVVDDRNSE
EFSIGLAAFN EAIDRYDADK NGNFYTYSFV VIKSRLYDFI RRNRKHNNVL PFSYIEESTR
VDERLLMSDA SGQFEKIEVR QELVSFEKSL KEFGISLEDL VLSSPKHKDS RLLLIKIARI
IADDDNMFRK LVEKKYIPMK EVLSRIKVNH KTIQRNRKFI IAVSLILRSN LYDLKEYVQG
FEREGKYHG
