SIGI4_ACET2
ID SIGI4_ACET2 Reviewed; 274 AA.
AC A3DCG2;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=RNA polymerase sigma factor SigI4 {ECO:0000305};
GN Name=sigI4 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_0403 {ECO:0000312|EMBL:ABN51641.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
RN [4]
RP INDUCTION.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=24782837; DOI=10.3389/fmicb.2014.00142;
RA Wei H., Fu Y., Magnusson L., Baker J.O., Maness P.C., Xu Q., Yang S.,
RA Bowersox A., Bogorad I., Wang W., Tucker M.P., Himmel M.E., Ding S.Y.;
RT "Comparison of transcriptional profiles of Clostridium thermocellum grown
RT on cellobiose and pretreated yellow poplar using RNA-Seq.";
RL Front. Microbiol. 5:142-142(2014).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism
CC (Probable). {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000305|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI4 (By similarity). Binding of the polysaccharide substrate to RsgI4
CC may lead to the release and activation of SigI4 (By similarity).
CC {ECO:0000250|UniProtKB:A3DBH0, ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI4. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Up-regulated in the presence of cellulose and further up-
CC regulated in the presence of both cellulose and xylan
CC (PubMed:20937888). Up-regulated in pretreated yellow poplar (PYP)-grown
CC cells (PubMed:24782837). {ECO:0000269|PubMed:20937888,
CC ECO:0000269|PubMed:24782837}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN51641.1; -; Genomic_DNA.
DR AlphaFoldDB; A3DCG2; -.
DR SMR; A3DCG2; -.
DR STRING; 203119.Cthe_0403; -.
DR EnsemblBacteria; ABN51641; ABN51641; Cthe_0403.
DR KEGG; cth:Cthe_0403; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; YSAEKGN; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..274
FT /note="RNA polymerase sigma factor SigI4"
FT /id="PRO_0000436518"
FT DNA_BIND 226..245
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 87..100
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 274 AA; 32452 MW; AFB43770C0FB96C4 CRC64;
MLNVQLKIFC HAALIFLEVV YLFEPNLIYG VKKREKKSRS DSINHIIIKI KNGDIELKEK
FIKKYKPYLL KIISSTLGRY VDPEVSEEYS VGLMAFNEAI DGFNPEINGN FTNYCNMVVN
HRIIDYIRKN KKYSNVIPFS YFEERNDFEE KYLVSDSHYL YENIEVKEEI LQFEQQLKQF
GITLEDLVMN SPKHKDSREL CISIARILSE NDKLFEKMIR KKCIPLSELM GLVNVHRKTV
ERNRKFIIAV SLILRSGLDE IKQFFRASEE RREK
