SIGI5_ACET2
ID SIGI5_ACET2 Reviewed; 245 AA.
AC A3DEX5;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=RNA polymerase sigma factor SigI5 {ECO:0000305};
GN Name=sigI5 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_1272 {ECO:0000312|EMBL:ABN52504.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism
CC (Probable). {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000305|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI5 (By similarity). Binding of the polysaccharide substrate to RsgI5
CC may lead to the release and activation of SigI5 (By similarity).
CC {ECO:0000250|UniProtKB:A3DBH0, ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI5. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Up-regulated in the presence of xylan.
CC {ECO:0000269|PubMed:20937888}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN52504.1; -; Genomic_DNA.
DR RefSeq; WP_003517500.1; NC_009012.1.
DR AlphaFoldDB; A3DEX5; -.
DR SMR; A3DEX5; -.
DR STRING; 203119.Cthe_1272; -.
DR PRIDE; A3DEX5; -.
DR EnsemblBacteria; ABN52504; ABN52504; Cthe_1272.
DR KEGG; cth:Cthe_1272; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; ASNICKR; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..245
FT /note="RNA polymerase sigma factor SigI5"
FT /id="PRO_0000436519"
FT DNA_BIND 202..221
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 60..73
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 245 AA; 28843 MW; C4D8B28ABFFF5ED8 CRC64;
MLFVSAIIDY AGETATHIVE KIKNGDKHLK EKFIKDYIPF ILNIVSSFYS SKTGDLKSSD
EYSIGLMAFN EAIEKFDVNK SKSFLKFAEM VIKKRMIDYF RKTSSIGRKE IPFSYFNSNN
ETEFRKKINN LDIEEEFNSY EFICELRDFS KKLESFGLSI NNLPDYMPKH KDSREMCINI
AKKIVENKSI FDKLKTKKYI QMKELSKIID VHPKTVERNR AFIICLCILF DNDYGNFKSY
LNKIF
