SIGI6_ACET2
ID SIGI6_ACET2 Reviewed; 253 AA.
AC A3DH98;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=RNA polymerase sigma factor SigI6 {ECO:0000305};
GN Name=sigI6 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_2120 {ECO:0000312|EMBL:ABN53327.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP FUNCTION, INTERACTION WITH RSGI6, AND INDUCTION.
RX PubMed=20937888; DOI=10.1073/pnas.1012175107;
RA Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer E.A.,
RA Sonenshein A.L., Shoham Y.;
RT "Clostridium thermocellum cellulosomal genes are regulated by
RT extracytoplasmic polysaccharides via alternative sigma factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18646-18651(2010).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=26731480; DOI=10.1371/journal.pone.0146316;
RA Munoz-Gutierrez I., Ortiz de Ora L., Rozman Grinberg I., Garty Y.,
RA Bayer E.A., Shoham Y., Lamed R., Borovok I.;
RT "Decoding biomass-sensing regulons of Clostridium thermocellum alternative
RT sigma-I factors in a heterologous Bacillus subtilis host system.";
RL PLoS ONE 11:E0146316-E0146316(2016).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (By similarity). This sigma factor is involved in regulation
CC of cellulosomal genes via an external polysaccharide-sensing mechanism
CC (Probable). Recognizes the predicted promoters associated with sigI6
CC itself, xyn11B, xyn10D, xyn10Z, xyn10Y, cel9V, cseP, sigI1, cipA, and
CC rsgI5 (PubMed:26731480). {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:26731480, ECO:0000305|PubMed:20937888}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI6 (By similarity). Binding of the polysaccharide substrate to RsgI6
CC may lead to the release and activation of SigI6 (By similarity).
CC {ECO:0000250|UniProtKB:A3DBH0, ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI6. {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:20937888}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Autoregulated (Probable). Up-regulated in the presence of
CC xylan (PubMed:20937888). {ECO:0000269|PubMed:20937888,
CC ECO:0000305|PubMed:26731480}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN53327.1; -; Genomic_DNA.
DR RefSeq; WP_003514231.1; NC_009012.1.
DR AlphaFoldDB; A3DH98; -.
DR SMR; A3DH98; -.
DR DIP; DIP-59453N; -.
DR IntAct; A3DH98; 1.
DR STRING; 203119.Cthe_2120; -.
DR EnsemblBacteria; ABN53327; ABN53327; Cthe_2120.
DR KEGG; cth:Cthe_2120; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; LEIRMEM; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..253
FT /note="RNA polymerase sigma factor SigI6"
FT /id="PRO_0000436520"
FT DNA_BIND 203..222
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 63..76
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 253 AA; 29969 MW; BC117974DFF78D7C CRC64;
MDWHFQGTND DREHTKRIII EYLNRIKAGD DSAREEFILR FRPFILKLVY KATDRHVEPE
NSEEYSVALL AFNEAINAYD EEKHSNFLVF SEQVINRRLI DYKRKNHKNK MVYPFSYFEN
EDIKLERTLS DADGNNAIER LEFTDEIRLF KSELASFDIT FKDLLSCTPK HRDSRELLIN
IAKKIASNDG LYEKLKKTKK LPTLELLKLA KVSRRTIERN KKYIIAVSLI LRSNLEIFKE
YAAGIQEKEV DLR
