SIGI7_ACET2
ID SIGI7_ACET2 Reviewed; 235 AA.
AC A3DIE4;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=RNA polymerase sigma factor SigI7 {ECO:0000305};
GN Name=sigI7 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_2521 {ECO:0000312|EMBL:ABN53723.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=24782837; DOI=10.3389/fmicb.2014.00142;
RA Wei H., Fu Y., Magnusson L., Baker J.O., Maness P.C., Xu Q., Yang S.,
RA Bowersox A., Bogorad I., Wang W., Tucker M.P., Himmel M.E., Ding S.Y.;
RT "Comparison of transcriptional profiles of Clostridium thermocellum grown
RT on cellobiose and pretreated yellow poplar using RNA-Seq.";
RL Front. Microbiol. 5:142-142(2014).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI7. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI7. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC -!- INDUCTION: Up-regulated in pretreated yellow poplar (PYP)-grown cells.
CC {ECO:0000269|PubMed:24782837}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR EMBL; CP000568; ABN53723.1; -; Genomic_DNA.
DR RefSeq; WP_020457849.1; NC_009012.1.
DR AlphaFoldDB; A3DIE4; -.
DR SMR; A3DIE4; -.
DR STRING; 203119.Cthe_2521; -.
DR EnsemblBacteria; ABN53723; ABN53723; Cthe_2521.
DR KEGG; cth:Cthe_2521; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_082361_0_0_9; -.
DR OMA; MYSVTIN; -.
DR OrthoDB; 1261602at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..235
FT /note="RNA polymerase sigma factor SigI7"
FT /id="PRO_0000436521"
FT DNA_BIND 191..210
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT MOTIF 49..62
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ SEQUENCE 235 AA; 27959 MW; 9357CC19AC183B09 CRC64;
MYSVTINQRV EAIKNNEEEI NLFVEEYKPF IAACTQKVVG RYVAYGQDDE LSIALMAFVE
TIRSYDVSKG NFLSFSQNVI KRRIIDYYRK EKKHSVVVNI NGHLEDEEEE TDLGIAMSID
KYSEEEISEY RRLELEQLKK ELKEWDISFF DLVNISPKHK RTKKIYSKII KFVLSRPDIM
EKIKQKKYLP VAEIEQSLKI PRKTIERARK YIITVVIIFT GDYEFIRDYV NWEVE
