ID   SIGI8_ACET2             Reviewed;         254 AA.
AC   A3DJP3;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=RNA polymerase sigma factor SigI8 {ECO:0000305};
GN   Name=sigI8 {ECO:0000303|PubMed:20487018};
GN   OrderedLocusNames=Cthe_2975 {ECO:0000312|EMBL:ABN54172.1};
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE.
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RX   PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA   Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA   Borovok I., Lamed R.;
RT   "The unique set of putative membrane-associated anti-sigma factors in
RT   Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT   sensing mechanism involved in gene regulation.";
RL   FEMS Microbiol. Lett. 308:84-93(2010).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC       RsgI8. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- SUBUNIT: Interacts with RsgI8. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR   EMBL; CP000568; ABN54172.1; -; Genomic_DNA.
DR   RefSeq; WP_003514757.1; NC_009012.1.
DR   AlphaFoldDB; A3DJP3; -.
DR   SMR; A3DJP3; -.
DR   STRING; 203119.Cthe_2975; -.
DR   EnsemblBacteria; ABN54172; ABN54172; Cthe_2975.
DR   KEGG; cth:Cthe_2975; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_082361_0_0_9; -.
DR   OMA; DFREQEQ; -.
DR   OrthoDB; 1261602at2; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02064; Sigma70_SigI; 1.
DR   InterPro; IPR014244; RNA_pol_sigma-I.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   PIRSF; PIRSF038953; SigI; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02895; spore_sigI; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..254
FT                   /note="RNA polymerase sigma factor SigI8"
FT                   /id="PRO_0000436522"
FT   DNA_BIND        209..228
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT   MOTIF           61..74
FT                   /note="Polymerase core binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ   SEQUENCE   254 AA;  30101 MW;  BDD84BBBB8755135 CRC64;
     MINLGSYNLL RHEKDSFLEI IRRIKDGDNL LRNKFIDDFK PFILKCVSQL VGKKNDLTQS
     DEYSIALIAF NEAIESYDLD KKTKFVSFSK QVIKRRLIDY LRSTKKNNVA VPFSYFNDCN
     SSFNDSSTGN FEEKFLYDRN SDYSIDFEAR EEIKNLELKI CEYKMTIEDL IECSPKHRDT
     IILCLNVANI IIEDESLYQM FNKRKTLPYK ELTERFNLCR RTLEKNRKFI TAMVLILKSD
     LEVLKKYIYD TLGR