ID   SIGI_BACAN              Reviewed;         240 AA.
AC   P0DO98;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=RNA polymerase sigma factor SigI {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000305};
GN   Name=sigI {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000303|PubMed:26744224};
GN   OrderedLocusNames=BAS3231 {ECO:0000312|EMBL:AAT55539.1};
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Sterne;
RX   PubMed=26744224; DOI=10.1099/mic.0.000236;
RA   Kim J.G., Wilson A.C.;
RT   "Loss of sigmaI affects heat-shock response and virulence gene expression
RT   in Bacillus anthracis.";
RL   Microbiology 162:564-574(2016).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor contributes to both stress response and
CC       virulence gene expression. {ECO:0000269|PubMed:26744224}.
CC   -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC       RsgI. {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000269|PubMed:26744224}.
CC   -!- SUBUNIT: Interacts with RsgI. {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064}.
CC   -!- INDUCTION: Part of the sigI-rsgI operon, which is induced by heat
CC       shock. Expressed from both the sigma I and sigma A promoters.
CC       Transcription from the sigma A promoter is likely more significant to
CC       virulence regulation. {ECO:0000269|PubMed:26744224}.
CC   -!- DISRUPTION PHENOTYPE: Disruption results in increased sensitivity to
CC       heat shock. Loss of SigI results in a coordinated decrease in
CC       expression of all three toxin subunits. {ECO:0000269|PubMed:26744224}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02064}.
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DR   EMBL; AE017225; AAT55539.1; -; Genomic_DNA.
DR   RefSeq; WP_003160074.1; NZ_QPKQ01000003.1.
DR   RefSeq; YP_029488.1; NC_005945.1.
DR   AlphaFoldDB; P0DO98; -.
DR   SMR; P0DO98; -.
DR   STRING; 260799.BAS3231; -.
DR   GeneID; 45023230; -.
DR   KEGG; bat:BAS3231; -.
DR   PATRIC; fig|260799.14.peg.3461; -.
DR   eggNOG; COG1191; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02064; Sigma70_SigI; 1.
DR   InterPro; IPR014244; RNA_pol_sigma-I.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   PIRSF; PIRSF038953; SigI; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02895; spore_sigI; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..240
FT                   /note="RNA polymerase sigma factor SigI"
FT                   /id="PRO_0000436443"
FT   DNA_BIND        194..213
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
FT   MOTIF           56..69
FT                   /note="Polymerase core binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02064"
SQ   SEQUENCE   240 AA;  28822 MW;  18555689A4875271 CRC64;
     MLSLVMKILR KPKIEDIVCN IQNNEEDKEA FIVQYQPFIR KSISSVCRRY ITEQDDEYSI
     GLFAFNEAIE QYSYKKGKSF LAFADLLIKR DVIDYIRKES KHNLVFLKED EQEEMLEMQV
     SLTEYMKEIE NGNRKEEILH FQSVLADFKI TFSELAKESP KHRDTREHLI EIVKVIIKEE
     EMMEELFRKK KLPLKHIEPR VRVSRKTLER HRKYIIAMCI IFANNYTYIL DYIRGGKHDE