SIGI_BACSU
ID SIGI_BACSU Reviewed; 251 AA.
AC O31654;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=RNA polymerase sigma factor SigI {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000305};
GN Name=sigI {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000303|PubMed:11157964};
GN Synonyms=ykoZ; OrderedLocusNames=BSU13450;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=11157964; DOI=10.1128/jb.183.4.1472-1475.2001;
RA Zuber U., Drzewiecki K., Hecker M.;
RT "Putative sigma factor sigI (ykoZ) of Bacillus subtilis is induced by heat
RT shock.";
RL J. Bacteriol. 183:1472-1475(2001).
RN [3]
RP INDUCTION, ACTIVITY REGULATION, AND INTERACTION WITH RSGI.
RC STRAIN=168;
RX PubMed=17185538; DOI=10.1099/mic.0.29239-0;
RA Asai K., Ootsuji T., Obata K., Matsumoto T., Fujita Y., Sadaie Y.;
RT "Regulatory role of rsgI in sigI expression in Bacillus subtilis.";
RL Microbiology 153:92-101(2007).
RN [4]
RP PROBABLE FUNCTION IN HEAT SHOCK RESPONSE.
RC STRAIN=168;
RX PubMed=18156261; DOI=10.1128/jb.01497-07;
RA Tseng C.-L., Shaw G.-C.;
RT "Genetic evidence for the actin homolog gene mreBH and the bacitracin
RT resistance gene bcrC as targets of the alternative sigma factor sigI of
RT Bacillus subtilis.";
RL J. Bacteriol. 190:1561-1567(2008).
RN [5]
RP FUNCTION IN COLD STRESS RESPONSE, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=19114499; DOI=10.1128/jb.01497-08;
RA Schirner K., Errington J.;
RT "The cell wall regulator sigmaI specifically suppresses the lethal
RT phenotype of mbl mutants in Bacillus subtilis.";
RL J. Bacteriol. 191:1404-1413(2009).
RN [6]
RP FUNCTION.
RC STRAIN=168;
RX PubMed=21541672; DOI=10.1007/s00203-011-0710-0;
RA Tseng C.L., Chen J.T., Lin J.H., Huang W.Z., Shaw G.C.;
RT "Genetic evidence for involvement of the alternative sigma factor SigI in
RT controlling expression of the cell wall hydrolase gene lytE and
RT contribution of LytE to heat survival of Bacillus subtilis.";
RL Arch. Microbiol. 193:677-685(2011).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=23199363; DOI=10.1111/mmi.12092;
RA Salzberg L.I., Powell L., Hokamp K., Botella E., Noone D., Devine K.M.;
RT "The WalRK (YycFG) and sigma(I) RsgI regulators cooperate to control CwlO
RT and LytE expression in exponentially growing and stressed Bacillus subtilis
RT cells.";
RL Mol. Microbiol. 87:180-195(2013).
RN [8]
RP INDUCTION.
RX PubMed=24125693; DOI=10.1016/j.resmic.2013.10.003;
RA Huang W.Z., Wang J.J., Chen H.J., Chen J.T., Shaw G.C.;
RT "The heat-inducible essential response regulator WalR positively regulates
RT transcription of sigI, mreBH and lytE in Bacillus subtilis under heat
RT stress.";
RL Res. Microbiol. 164:998-1008(2013).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of cell wall
CC metabolism in response to heat stress. Acts by regulating the
CC expression of genes such as bcrC, mreBH and lytE (PubMed:18156261,
CC PubMed:21541672, PubMed:23199363). Also plays a role in survival at low
CC temperatures (PubMed:19114499). {ECO:0000269|PubMed:18156261,
CC ECO:0000269|PubMed:19114499, ECO:0000269|PubMed:21541672,
CC ECO:0000269|PubMed:23199363}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI. Upon exposure to heat, SigI is released from RsgI and activated.
CC Transient heat activation of SigI may depend upon DnaK chaperone.
CC {ECO:0000269|PubMed:17185538}.
CC -!- SUBUNIT: Interacts with RsgI. {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000269|PubMed:17185538}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02064,
CC ECO:0000305}.
CC -!- INDUCTION: Part of the sigI-rsgI operon, which is transiently induced
CC by heat stress. Expression is positively autoregulated via the sigma-I
CC promoter (PubMed:11157964, PubMed:17185538). In exponentially growing
CC cells, expression is regulated by the WalRK two-component system, which
CC represses the sigma-I promoter and activates the sigma-A promoter,
CC leading to the formation of a basal level of SigI (PubMed:23199363).
CC WalRK can also positively and directly regulate transcription of the
CC operon under heat stress through a binding site located upstream of the
CC sigma-I promoter (PubMed:24125693). Repressed by glucose
CC (PubMed:17185538). {ECO:0000269|PubMed:11157964,
CC ECO:0000269|PubMed:17185538, ECO:0000269|PubMed:23199363,
CC ECO:0000269|PubMed:24125693}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene exhibit normal cell
CC morphology and growth rate at 37 degrees Celsius, but cannot grow at
CC high temperature. {ECO:0000269|PubMed:11157964,
CC ECO:0000269|PubMed:19114499}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000305}.
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DR EMBL; AL009126; CAB13218.1; -; Genomic_DNA.
DR PIR; D69861; D69861.
DR RefSeq; NP_389228.1; NC_000964.3.
DR RefSeq; WP_003245855.1; NZ_CP053102.1.
DR AlphaFoldDB; O31654; -.
DR SMR; O31654; -.
DR IntAct; O31654; 1.
DR STRING; 224308.BSU13450; -.
DR PaxDb; O31654; -.
DR PRIDE; O31654; -.
DR EnsemblBacteria; CAB13218; CAB13218; BSU_13450.
DR GeneID; 939363; -.
DR KEGG; bsu:BSU13450; -.
DR PATRIC; fig|224308.179.peg.1460; -.
DR eggNOG; COG1191; Bacteria.
DR OMA; YSAEKGN; -.
DR PhylomeDB; O31654; -.
DR BioCyc; BSUB:BSU13450-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR PANTHER; PTHR30385:SF6; PTHR30385:SF6; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02895; spore_sigI; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..251
FT /note="RNA polymerase sigma factor SigI"
FT /id="PRO_0000379970"
FT DNA_BIND 206..225
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000305"
FT MOTIF 61..74
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02064, ECO:0000305"
SQ SEQUENCE 251 AA; 29193 MW; B0F8A2CDAC136137 CRC64;
MKPVLSLLFK LGKKKQTLEK AVESIQKGNK DLQNELIQQY KPFIAKTVSS VCKRYIDEKD
DEFSIGLIAF NEAIEKYSPE KGNSLLAFAE LIIKRKVIDY IRKEARSAQN INIDLQEGDD
QESSQSLIEA ELSIDEYRRQ IEQEQRREEI LYFQKQLKDY GLSFKELLEN SPKHTDARQN
AIKVAMTLVE HEELAAILYT KKQLPVKQLE QLVSVSRKTI ERNRKYIIAM CIIITGDYIY
LKDYLKGVLH S
