ID   SIGI_MYCTO              Reviewed;         290 AA.
AC   P9WGH2; L7N4U5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Probable ECF RNA polymerase sigma factor SigI;
DE            Short=ECF sigma factor SigI;
DE   AltName: Full=Alternative RNA polymerase sigma factor SigI;
DE   AltName: Full=RNA polymerase sigma-I factor;
DE            Short=Sigma-I factor;
GN   Name=sigI; OrderedLocusNames=MT1226;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Extracytoplasmic function (ECF) sigma factors are held in an
CC       inactive form by a cognate anti-sigma factor until released, although
CC       no anti-sigma factor is known for this protein (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC       formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC       omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC       transcription. {ECO:0000250}.
CC   -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC       interaction with the -10 element in promoter DNA, and plays an
CC       important role in melting the double-stranded DNA and the formation of
CC       the transcription bubble. The sigma-70 factor domain-2 mediates
CC       interaction with the RNA polymerase subunits RpoB and RpoC (By
CC       similarity). {ECO:0000250}.
CC   -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC       H) motif that mediates interaction with the -35 element in promoter
CC       DNA. The domain also mediates interaction with the RNA polymerase
CC       subunit RpoA (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45483.1; -; Genomic_DNA.
DR   PIR; E70877; E70877.
DR   RefSeq; WP_003406207.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGH2; -.
DR   SMR; P9WGH2; -.
DR   EnsemblBacteria; AAK45483; AAK45483; MT1226.
DR   KEGG; mtc:MT1226; -.
DR   PATRIC; fig|83331.31.peg.1325; -.
DR   HOGENOM; CLU_047691_22_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Sigma factor; Transcription; Transcription regulation.
FT   CHAIN           1..290
FT                   /note="Probable ECF RNA polymerase sigma factor SigI"
FT                   /id="PRO_0000428362"
FT   DNA_BIND        137..156
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          11..74
FT                   /note="Sigma-70 factor domain-2"
FT   REGION          110..162
FT                   /note="Sigma-70 factor domain-4_2"
FT   MOTIF           34..37
FT                   /note="Polymerase core binding"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   290 AA;  31431 MW;  5120BD4F8A374F8C CRC64;
     MSQHDPVSAA WRAHRAYLVD LAFRMVGDIG VAEDMVQEAF SRLLRAPVGD IDDERGWLIV
     VTSRLCLDHI KSASTRRERP QDIAAWHDGD ASVSSVDPAD RVTLDDEVRL ALLIMLERLG
     PAERVVFVLH EIFGLPYQQI ATTIGSQAST CRQLAHRARR KINESRIAAS VEPAQHRVVT
     RAFIEACSNG DLDTLLEVLD PGVAGEIDAR KGVVVVGADR VGPTILRHWS HPATVLVAQP
     VCGQPAVLAF VNRALAGVLA LSIEAGKITK IHVLVQPSTL DPLRAELGGG