SIGI_MYCTU
ID SIGI_MYCTU Reviewed; 290 AA.
AC P9WGH3; L7N4U5;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Probable ECF RNA polymerase sigma factor SigI;
DE Short=ECF sigma factor SigI;
DE AltName: Full=Alternative RNA polymerase sigma factor SigI;
DE AltName: Full=RNA polymerase sigma-I factor;
DE Short=Sigma-I factor;
GN Name=sigI; OrderedLocusNames=Rv1189;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=10027986; DOI=10.1046/j.1365-2958.1999.01212.x;
RA Manganelli R., Dubnau E., Tyagi S., Kramer F.R., Smith I.;
RT "Differential expression of 10 sigma factor genes in Mycobacterium
RT tuberculosis.";
RL Mol. Microbiol. 31:715-724(1999).
RN [3]
RP INDUCTION BY SIGJ.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=18248429; DOI=10.1111/j.1574-6968.2007.01054.x;
RA Homerova D., Halgasova L., Kormanec J.;
RT "Cascade of extracytoplasmic function sigma factors in Mycobacterium
RT tuberculosis: identification of a sigmaJ-dependent promoter upstream of
RT sigI.";
RL FEMS Microbiol. Lett. 280:120-126(2008).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Extracytoplasmic function (ECF) sigma factors are held in an
CC inactive form by a cognate anti-sigma factor until released, although
CC no anti-sigma factor is known for this protein (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC transcription. {ECO:0000250}.
CC -!- INDUCTION: Constitutively expressed at a low level under all conditions
CC tested. Transcribed under control of SigJ.
CC {ECO:0000269|PubMed:10027986, ECO:0000269|PubMed:18248429}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP43945.1; -; Genomic_DNA.
DR PIR; E70877; E70877.
DR RefSeq; NP_215705.1; NC_000962.3.
DR RefSeq; WP_003406207.1; NZ_NVQJ01000025.1.
DR AlphaFoldDB; P9WGH3; -.
DR SMR; P9WGH3; -.
DR STRING; 83332.Rv1189; -.
DR PaxDb; P9WGH3; -.
DR DNASU; 886079; -.
DR GeneID; 886079; -.
DR KEGG; mtu:Rv1189; -.
DR TubercuList; Rv1189; -.
DR eggNOG; COG1595; Bacteria.
DR OMA; LAYQMLG; -.
DR PhylomeDB; P9WGH3; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IBA:GO_Central.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IEP:MTBBASE.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..290
FT /note="Probable ECF RNA polymerase sigma factor SigI"
FT /id="PRO_0000423647"
FT DNA_BIND 137..156
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 11..74
FT /note="Sigma-70 factor domain-2"
FT REGION 110..162
FT /note="Sigma-70 factor domain-4_2"
FT MOTIF 34..37
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255"
SQ SEQUENCE 290 AA; 31431 MW; 5120BD4F8A374F8C CRC64;
MSQHDPVSAA WRAHRAYLVD LAFRMVGDIG VAEDMVQEAF SRLLRAPVGD IDDERGWLIV
VTSRLCLDHI KSASTRRERP QDIAAWHDGD ASVSSVDPAD RVTLDDEVRL ALLIMLERLG
PAERVVFVLH EIFGLPYQQI ATTIGSQAST CRQLAHRARR KINESRIAAS VEPAQHRVVT
RAFIEACSNG DLDTLLEVLD PGVAGEIDAR KGVVVVGADR VGPTILRHWS HPATVLVAQP
VCGQPAVLAF VNRALAGVLA LSIEAGKITK IHVLVQPSTL DPLRAELGGG
