SIGK_MYCTE
ID SIGK_MYCTE Reviewed; 187 AA.
AC H8EVT0;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 1.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=ECF RNA polymerase sigma factor SigK;
DE Short=ECF sigma factor SigK;
DE AltName: Full=Alternative RNA polymerase sigma factor SigK;
DE AltName: Full=RNA polymerase sigma-K factor;
DE Short=Sigma-K factor;
GN Name=sigK; OrderedLocusNames=ERDMAN_0488;
OS Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=652616;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=22535945; DOI=10.1128/jb.00353-12;
RA Miyoshi-Akiyama T., Matsumura K., Iwai H., Funatogawa K., Kirikae T.;
RT "Complete annotated genome sequence of Mycobacterium tuberculosis Erdman.";
RL J. Bacteriol. 194:2770-2770(2012).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=20545848; DOI=10.1111/j.1365-2958.2010.07232.x;
RA Sklar J.G., Makinoshima H., Schneider J.S., Glickman M.S.;
RT "M. tuberculosis intramembrane protease Rip1 controls transcription through
RT three anti-sigma factor substrates.";
RL Mol. Microbiol. 77:605-617(2010).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Extracytoplasmic function (ECF) sigma factors are held in an
CC inactive form by an anti-sigma factor until released by regulated
CC intramembrane proteolysis (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC transcription. Interacts (via sigma-70 factor domain 4) with anti-
CC sigma-K factor RsKA (By similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-
CC sigma factors prevents interaction of sigma factors with the RNA
CC polymerase catalytic core (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Loss of phenanthroline induction of katG.
CC {ECO:0000269|PubMed:20545848}.
CC -!- MISCELLANEOUS: Extracytoplasmic function (ECF) sigma factors are held
CC in an inactive form by an anti-sigma factor until released by regulated
CC intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic
CC signal triggers a concerted proteolytic cascade to transmit information
CC and elicit cellular responses. The membrane-spanning anti-sigma factor
CC is first cut extracytoplasmically (site-1 protease, S1P), then within
CC the membrane itself (site-2 protease, S2P, Rip1), while cytoplasmic
CC proteases finish degrading the regulatory protein, liberating SigK
CC (Probable). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
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DR EMBL; AP012340; BAL64304.1; -; Genomic_DNA.
DR RefSeq; WP_003402246.1; NZ_KK339487.1.
DR AlphaFoldDB; H8EVT0; -.
DR SMR; H8EVT0; -.
DR EnsemblBacteria; BAL64304; BAL64304; ERDMAN_0488.
DR KEGG; mtn:ERDMAN_0488; -.
DR PATRIC; fig|652616.3.peg.494; -.
DR HOGENOM; CLU_047691_9_3_11; -.
DR Proteomes; UP000007568; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE 3: Inferred from homology;
KW DNA-binding; Sigma factor; Transcription; Transcription regulation.
FT CHAIN 1..187
FT /note="ECF RNA polymerase sigma factor SigK"
FT /id="PRO_0000422692"
FT DNA_BIND 155..174
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 30..96
FT /note="Sigma-70 factor domain-2"
FT REGION 133..182
FT /note="Sigma-70 factor domain-4"
FT MOTIF 53..56
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 187 AA; 21035 MW; 2156CEB813620F02 CRC64;
MTGPPRLSSD LDALLRRVAG HDQAAFAEFY DHTKSRVYGL VMRVLRDTGY SEETTQEIYL
EVWRNASEFD SAKGSALAWL LTMAHRRAVD RVRCEQAGNQ REVRYGAANV DPASDVVADL
AIAGDERRRV TECLKALTDT QRQCIELAYY GGLTYVEVSR RLAANLSTIK SRMRDALRSL
RNCLDVS
