BH100_ARATH
ID BH100_ARATH Reviewed; 242 AA.
AC Q9ZVB5; Q3E7M6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcription factor bHLH100;
DE AltName: Full=Basic helix-loop-helix protein 100;
DE Short=AtbHLH100;
DE Short=bHLH 100;
DE AltName: Full=Transcription factor EN 7;
DE AltName: Full=bHLH transcription factor bHLH100;
GN Name=BHLH100; Synonyms=EN7; OrderedLocusNames=At2g41240;
GN ORFNames=F13H10.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-242 (ISOFORM 2), TISSUE SPECIFICITY,
RP INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP INDUCTION.
RX PubMed=17516080; DOI=10.1007/s00425-007-0535-x;
RA Wang H.-Y., Klatte M., Jakoby M., Baeumlein H., Weisshaar B., Bauer P.;
RT "Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes
RT in Arabidopsis thaliana.";
RL Planta 226:897-908(2007).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=18088336; DOI=10.1111/j.1365-3040.2007.01764.x;
RA van de Mortel J.E., Schat H., Moerland P.D., Ver Loren van Themaat E.,
RA van der Ent S., Blankestijn H., Ghandilyan A., Tsiatsiani S., Aarts M.G.M.;
RT "Expression differences for genes involved in lignin, glutathione and
RT sulphate metabolism in response to cadmium in Arabidopsis thaliana and the
RT related Zn/Cd-hyperaccumulator Thlaspi caerulescens.";
RL Plant Cell Environ. 31:301-324(2008).
CC -!- FUNCTION: Plays a role in metal homeostasis. Confers tolerance to high
CC zinc (Zn) and nickel (Ni). {ECO:0000269|PubMed:18088336}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q9ZVB5; Q0V7X4: FIT; NbExp=3; IntAct=EBI-15198769, EBI-1640543;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZVB5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZVB5-2; Sequence=VSP_036095;
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and
CC stems. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Up regulated by iron deficiency in roots and leaves, as well
CC as by nickel, high zinc or high copper treatments. Repressed by heat
CC treatment, high iron, low copper and low zinc treatments.
CC {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:17516080,
CC ECO:0000269|PubMed:18088336}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
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DR EMBL; AC005662; AAC78547.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09951.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09952.1; -; Genomic_DNA.
DR EMBL; AY074635; AAL69451.1; -; mRNA.
DR EMBL; AF488626; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; E84839; E84839.
DR RefSeq; NP_181657.1; NM_129689.2. [Q9ZVB5-1]
DR RefSeq; NP_850349.1; NM_180018.1. [Q9ZVB5-2]
DR AlphaFoldDB; Q9ZVB5; -.
DR SMR; Q9ZVB5; -.
DR BioGRID; 4060; 6.
DR IntAct; Q9ZVB5; 6.
DR STRING; 3702.AT2G41240.1; -.
DR PaxDb; Q9ZVB5; -.
DR PRIDE; Q9ZVB5; -.
DR EnsemblPlants; AT2G41240.1; AT2G41240.1; AT2G41240. [Q9ZVB5-1]
DR EnsemblPlants; AT2G41240.2; AT2G41240.2; AT2G41240. [Q9ZVB5-2]
DR GeneID; 818723; -.
DR Gramene; AT2G41240.1; AT2G41240.1; AT2G41240. [Q9ZVB5-1]
DR Gramene; AT2G41240.2; AT2G41240.2; AT2G41240. [Q9ZVB5-2]
DR KEGG; ath:AT2G41240; -.
DR Araport; AT2G41240; -.
DR TAIR; locus:2040287; AT2G41240.
DR eggNOG; ENOG502RXMR; Eukaryota.
DR HOGENOM; CLU_089779_1_1_1; -.
DR InParanoid; Q9ZVB5; -.
DR OMA; TNQNAKP; -.
DR PhylomeDB; Q9ZVB5; -.
DR PRO; PR:Q9ZVB5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZVB5; baseline and differential.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010106; P:cellular response to iron ion starvation; IEP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..242
FT /note="Transcription factor bHLH100"
FT /id="PRO_0000358789"
FT DOMAIN 61..113
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT VAR_SEQ 96
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12679534"
FT /id="VSP_036095"
FT CONFLICT 215
FT /note="Q -> R (in Ref. 4; AF488626)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 242 AA; 27217 MW; 2E3FDE4D3653C1E3 CRC64;
MCALVPPLYP NFGWPCGDHS FYETDDVSNT FLDFPLPDLT VTHENVSSEN NRTLLDNPVV
MKKLNHNASE RERRKKINTM FSSLRSCLPP TNQTKKLSVS ATVSQALKYI PELQEQVKKL
MKKKEELSFQ ISGQRDLVYT DQNSKSEEGV TSYASTVSST RLSETEVMVQ ISSLQTEKCS
FGNVLSGVEE DGLVLVGASS SRSHGERLFY SMHLQIKNGQ VNSEELGDRL LYLYEKCGHS
FT
