BH101_ARATH
ID BH101_ARATH Reviewed; 240 AA.
AC Q9FYE6; A0MFD6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Transcription factor bHLH101;
DE AltName: Full=Basic helix-loop-helix protein 101;
DE Short=AtbHLH101;
DE Short=bHLH 101;
DE AltName: Full=Transcription factor EN 10;
DE AltName: Full=bHLH transcription factor bHLH101;
GN Name=BHLH101; Synonyms=EN10; OrderedLocusNames=At5g04150;
GN ORFNames=F21E1.70;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP INDUCTION.
RX PubMed=17516080; DOI=10.1007/s00425-007-0535-x;
RA Wang H.-Y., Klatte M., Jakoby M., Baeumlein H., Weisshaar B., Bauer P.;
RT "Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes
RT in Arabidopsis thaliana.";
RL Planta 226:897-908(2007).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q9FYE6; Q0V7X4: FIT; NbExp=4; IntAct=EBI-15195485, EBI-1640543;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Flowers. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Up regulated by iron deficiency in roots and leaves, as well
CC as by nickel, high zinc or high copper treatments. Repressed by high
CC iron, low copper and low zinc treatments.
CC {ECO:0000269|PubMed:17516080}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28679.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ519810; CAD58594.1; -; mRNA.
DR EMBL; AL391716; CAC05497.1; -; Genomic_DNA.
DR EMBL; CP002688; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQ446915; ABE66128.1; -; mRNA.
DR EMBL; DQ653262; ABK28679.1; ALT_SEQ; mRNA.
DR AlphaFoldDB; Q9FYE6; -.
DR SMR; Q9FYE6; -.
DR BioGRID; 15573; 3.
DR IntAct; Q9FYE6; 2.
DR STRING; 3702.AT5G04150.1; -.
DR PaxDb; Q9FYE6; -.
DR PRIDE; Q9FYE6; -.
DR EnsemblPlants; AT5G04150.1; AT5G04150.1; AT5G04150.
DR Gramene; AT5G04150.1; AT5G04150.1; AT5G04150.
DR Araport; AT5G04150; -.
DR TAIR; locus:2146663; AT5G04150.
DR eggNOG; ENOG502RXMR; Eukaryota.
DR HOGENOM; CLU_089779_1_0_1; -.
DR InParanoid; Q9FYE6; -.
DR PhylomeDB; Q9FYE6; -.
DR PRO; PR:Q9FYE6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FYE6; baseline and differential.
DR Genevisible; Q9FYE6; AT.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010106; P:cellular response to iron ion starvation; IEP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..240
FT /note="Transcription factor bHLH101"
FT /id="PRO_0000358790"
FT DOMAIN 65..117
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 240 AA; 27717 MW; 780D6D5C3AFED9A7 CRC64;
MEYPWLQSQV HSFSPTLHFP SFLHPLDDSK SHNINLHHMS LSHSNNTNSN NNNYQEEDRG
AVVLEKKLNH NASERDRRRK LNALYSSLRA LLPLSDQKRK LSIPMTVARV VKYIPEQKQE
LQRLSRRKEE LLKRISRKTH QEQLRNKAMM DSIDSSSSQR IAANWLTDTE IAVQIATSKW
TSVSDMLLRL EENGLNVISV SSSVSSTARI FYTLHLQMRG DCKVRLEELI NGMLLGLRQS
