ID   SIGNS_REOVD             Reviewed;         366 AA.
AC   P03526; A4ZY28; Q9ENN1;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   07-OCT-2020, entry version 74.
DE   RecName: Full=Protein sigma-NS;
DE            Short=SigmaNS;
GN   Name=S3;
OS   Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus.
OX   NCBI_TaxID=10886;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6312421; DOI=10.1093/nar/11.18.6399;
RA   Richardson M.A., Furuichi Y.;
RT   "Nucleotide sequence of reovirus genome segment S3, encoding non-structural
RT   protein sigma NS.";
RL   Nucleic Acids Res. 11:6399-6408(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=Mutant tsE320;
RX   PubMed=11152519; DOI=10.1128/jvi.75.3.1459-1475.2001;
RA   Becker M.M., Goral M.I., Hazelton P.R., Baer G.S., Rodgers S.E.,
RA   Brown E.G., Coombs K.M., Dermody T.S.;
RT   "Reovirus sigmaNS protein is required for nucleation of viral assembly
RT   complexes and formation of viral inclusions.";
RL   J. Virol. 75:1459-1475(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Infectious clone;
RX   PubMed=18005692; DOI=10.1016/j.chom.2007.03.003;
RA   Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M.,
RA   Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D.,
RA   Wilson G.J., Chappell J.D., Dermody T.S.;
RT   "A plasmid-based reverse genetics system for animal double-stranded RNA
RT   viruses.";
RL   Cell Host Microbe 1:147-157(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-17.
RX   PubMed=6927854; DOI=10.1016/0042-6822(82)90170-2;
RA   Antczak J.B., Chmelo R.A., Pickup D.J., Joklik W.K.;
RT   "Sequence at both termini of the 10 genes of reovirus serotype 3 (strain
RT   Dearing).";
RL   Virology 121:307-319(1982).
CC   -!- FUNCTION: Protein that binds to ssRNA and may be involved in genome
CC       packaging. {ECO:0000250, ECO:0000269|PubMed:11152519}.
CC   -!- SUBUNIT: Homomultimer. Interacts with protein mu-NS; in viral
CC       inclusions. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the orthoreovirus sigma-NS protein family.
CC       {ECO:0000305}.
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DR   EMBL; X01627; CAA25768.1; -; Genomic_RNA.
DR   EMBL; AF076293; AAG02124.1; -; mRNA.
DR   EMBL; EF494443; ABP48921.1; -; Genomic_RNA.
DR   EMBL; J02330; AAA47280.1; -; Genomic_RNA.
DR   PIR; A04126; MNXRSD.
DR   PIR; C27401; C27401.
DR   Proteomes; UP000006373; Genome.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0003727; F:single-stranded RNA binding; IEA:InterPro.
DR   InterPro; IPR002507; Reovirus_polyG_pol.
DR   Pfam; PF01518; PolyG_pol; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..366
FT                   /note="Protein sigma-NS"
FT                   /id="PRO_0000222759"
FT   REGION          1..11
FT                   /note="Important for ssRNA-binding and formation of
FT                   complexes"
FT                   /evidence="ECO:0000250"
FT   VARIANT         260
FT                   /note="M -> T (in strain: Mutant tsE320)"
SQ   SEQUENCE   366 AA;  41057 MW;  201A6223C4290D7D CRC64;
     MASSLRAAIS KIKRDDVGQQ VCPNYVMLRS SVTTKVVRNV VEYQIRTGGF FSCLAMLRPL
     QYAKRERLLG QRNLERISTR DILQTRDLHS LCMPTPDAPM SNHQASTMRE LICSYFKVDH
     ADGLKYIPMD ERYSPSSLAR LFTMGMAGLH ITTEPSYKRV PIMHLAADLD CMTLALPYMI
     TLDGDTVVPV APTLSAEQLL DDGLKGLACM DISYGCEVDA NSRPAGDQSM DSSRCINELY
     CEETAEAICV LKTCLVLNCM QFKLEMDDLA HNAAELDKIQ MMIPFSERVF RMASSFATID
     AQCFRFCVMM KDKNLKIDMR ETTRLWTRSA SDDSVATSSL SISLDRGRWV AADASDARLL
     VFPIRV