ID   SIGNS_REOVL             Reviewed;         366 AA.
AC   P07940;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   12-AUG-2020, entry version 70.
DE   RecName: Full=Protein sigma-NS;
DE            Short=SigmaNS;
GN   Name=S3;
OS   Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus.
OX   NCBI_TaxID=10884;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3767989; DOI=10.1016/s0006-291x(86)80067-5;
RA   George C.X., Atwater J.A., Samuel C.E.;
RT   "Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning
RT   and nucleotide sequence of the reovirus serotype 1 Lang strain s3 mRNA
RT   which encodes the nonstructural RNA-binding protein sigma NS.";
RL   Biochem. Biophys. Res. Commun. 139:845-851(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3686825; DOI=10.1016/0042-6822(87)90125-5;
RA   Wiener J.R., Joklik W.K.;
RT   "Comparison of the reovirus serotype 1, 2, and 3 S3 genome segments
RT   encoding the nonstructural protein sigma NS.";
RL   Virology 161:332-339(1987).
RN   [3]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=9448684; DOI=10.1006/viro.1997.8905;
RA   Gillian A.L., Nibert M.L.;
RT   "Amino terminus of reovirus nonstructural protein sigma NS is important for
RT   ssRNA binding and nucleoprotein complex formation.";
RL   Virology 240:1-11(1998).
RN   [4]
RP   INTERACTION WITH PROTEIN MU-NS.
RX   PubMed=12663763; DOI=10.1128/jvi.77.8.4566-4576.2003;
RA   Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.;
RT   "Reovirus sigma NS protein localizes to inclusions through an association
RT   requiring the mu NS amino terminus.";
RL   J. Virol. 77:4566-4576(2003).
CC   -!- FUNCTION: Protein that binds to ssRNA and may be involved in genome
CC       packaging. {ECO:0000269|PubMed:9448684}.
CC   -!- SUBUNIT: Homomultimer (Probable). Interacts with protein mu-NS; in
CC       viral inclusions. {ECO:0000269|PubMed:12663763,
CC       ECO:0000269|PubMed:9448684, ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orthoreovirus sigma-NS protein family.
CC       {ECO:0000305}.
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DR   EMBL; M14325; AAA47273.1; -; Genomic_RNA.
DR   EMBL; M18389; AAA47281.1; -; Genomic_RNA.
DR   PIR; A25068; MNXRST.
DR   Proteomes; UP000007253; Genome.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0003727; F:single-stranded RNA binding; IEA:InterPro.
DR   InterPro; IPR002507; Reovirus_polyG_pol.
DR   Pfam; PF01518; PolyG_pol; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..366
FT                   /note="Protein sigma-NS"
FT                   /id="PRO_0000222761"
FT   REGION          1..11
FT                   /note="Important for ssRNA-binding and formation of
FT                   complexes"
FT   CONFLICT        250
FT                   /note="V -> I (in Ref. 2; AAA47281)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   366 AA;  41189 MW;  B9172A6A9251CC9A CRC64;
     MASSLRAAIS KIKRDDVGQQ VCPNYVMLRS SVTTKVVRNV VEYQIRTGGF FSCLAMLRPL
     QYAKRERLLG QRNLERISTR DILQTRDLHS LCMPTPDAPM SNHQAATMRE LICSYFKVDH
     TDGLKYIPMD ERYSPSSLAR LFTMGMAGLH ITTEPSYKRV PIMHLAADLD CMTLALPYMI
     TLDGDTVVPV APTLSAEQLL DDGLKGLACM DISYGCEVDA SNRSAGDQSM DSSRCINELY
     CEETAEAICV LKTCLVLNCM QFKLEMDDLA HNATELDKIQ MMIPFSERVF RMASSFATID
     AQCFRFCVMM KDKNLKIDMR ETMRLWTRSA LDDSVVTSSL SISLDRGRWV AADATDARLL
     VFPIRV