ID   SIGO_BACSU              Reviewed;         176 AA.
AC   O34843; Q7B2K5;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=RNA polymerase sigma factor SigO;
GN   Name=sigO; Synonyms=yvrI; OrderedLocusNames=BSU33230;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA   Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT   "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT   chromosome containing genes involved in metal ion uptake and a putative
RT   sigma factor.";
RL   Microbiology 144:1593-1600(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 174.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION AS A SIGMA FACTOR, INTERACTION WITH RNA POLYMERASE, AND INDUCTION.
RC   STRAIN=168 / CU1065;
RX   PubMed=18573182; DOI=10.1111/j.1365-2958.2008.06331.x;
RA   MacLellan S.R., Wecke T., Helmann J.D.;
RT   "A previously unidentified sigma factor and two accessory proteins regulate
RT   oxalate decarboxylase expression in Bacillus subtilis.";
RL   Mol. Microbiol. 69:954-967(2008).
RN   [5]
RP   FUNCTION IN OXDC INDUCTION, AND INDUCTION.
RC   STRAIN=168 / CU1065;
RX   PubMed=19047353; DOI=10.1128/jb.01435-08;
RA   MacLellan S.R., Helmann J.D., Antelmann H.;
RT   "The yvrI alternative sigma factor is essential for acid stress induction
RT   of oxalate decarboxylase in Bacillus subtilis.";
RL   J. Bacteriol. 191:931-939(2009).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Together with its coactivator RsoA, positively regulates the
CC       expression of at least three operons, including oxdC-yvrL, sigO-rsoA
CC       and yvrJ. Required for the acid stress-dependent induction of the
CC       oxalate decarboxylase oxdC. {ECO:0000269|PubMed:18573182,
CC       ECO:0000269|PubMed:19047353}.
CC   -!- SUBUNIT: Interacts with RNA polymerase. {ECO:0000269|PubMed:18573182}.
CC   -!- INTERACTION:
CC       O34843; C0H3R4: rsoA; NbExp=2; IntAct=EBI-15816883, EBI-15816929;
CC   -!- INDUCTION: In the mid-exponential phase of growth under acidic
CC       conditions. Positively autoregulated, in the presence of RsoA. Down-
CC       regulated by YvrL under nonstress conditions.
CC       {ECO:0000269|PubMed:18573182, ECO:0000269|PubMed:19047353}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA11728.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ223978; CAA11728.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAB15313.2; -; Genomic_DNA.
DR   RefSeq; NP_391203.2; NC_000964.3.
DR   RefSeq; WP_003243707.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O34843; -.
DR   SMR; O34843; -.
DR   DIP; DIP-49019N; -.
DR   IntAct; O34843; 3.
DR   STRING; 224308.BSU33230; -.
DR   PaxDb; O34843; -.
DR   PRIDE; O34843; -.
DR   EnsemblBacteria; CAB15313; CAB15313; BSU_33230.
DR   GeneID; 935983; -.
DR   KEGG; bsu:BSU33230; -.
DR   PATRIC; fig|224308.43.peg.3483; -.
DR   eggNOG; COG1191; Bacteria.
DR   OMA; INEGLAM; -.
DR   BioCyc; BSUB:BSU33230-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..176
FT                   /note="RNA polymerase sigma factor SigO"
FT                   /id="PRO_0000377726"
FT   DNA_BIND        139..158
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           30..43
FT                   /note="Polymerase core binding"
FT   CONFLICT        174
FT                   /note="G -> R (in Ref. 1; CAA11728)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   176 AA;  20757 MW;  70B869300CFAE709 CRC64;
     MKHPIVKHFL SNPQHYRLFK NVMESPNEKD ARSLDELFKQ FYKEIRIVKY MNSMIRIFSI
     DFDKRVRKNQ KRYPLTVDHP EAGDRLSSET GSDAFEEFLD RQDDLSQHVQ DYQLYQAIQK
     LTDKQKSVLT KVYLHGATMQ EIADSLGESR QNISNIHKKG LENIRKQLAA QKKGEK