ID   SIGS_STAA8              Reviewed;         156 AA.
AC   Q2FXF2; Q8GQH6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=RNA polymerase sigma factor SigS;
GN   Name=sigS; OrderedLocusNames=SAOUHSC_01897;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Cramton S.E., Richard C., Oberfeld B., Pfitzner U., Goetz F.;
RT   "Identification and characterization of a novel glucosaminidase important
RT   for biofilm formation in Staphylococcus aureus.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [3]
RP   FUNCTION AS A SIGMA FACTOR, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19050758; DOI=10.1371/journal.pone.0003844;
RA   Shaw L.N., Lindholm C., Prajsnar T.K., Miller H.K., Brown M.C., Golonka E.,
RA   Stewart G.C., Tarkowski A., Potempa J.;
RT   "Identification and characterization of sigma(S), a novel component of the
RT   Staphylococcus aureus stress and virulence responses.";
RL   PLoS ONE 3:E3844-E3844(2008).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Sigma-S contributes to the protection against external
CC       stress, thus playing a role in cellular fitness and survival.
CC       {ECO:0000269|PubMed:19050758}.
CC   -!- INDUCTION: Autoregulated. {ECO:0000269|PubMed:19050758}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene display a decrease in
CC       viability during heat shock, long term survival and starvation
CC       experiments. They also exhibit significantly reduced virulence in a
CC       murine model of septic arthritis infection and decreased capacity to
CC       colonize host tissues. {ECO:0000269|PubMed:19050758}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN32728.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF422190; AAN32728.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP000253; ABD30960.1; -; Genomic_DNA.
DR   RefSeq; WP_000671063.1; NZ_LS483365.1.
DR   RefSeq; YP_500398.1; NC_007795.1.
DR   AlphaFoldDB; Q2FXF2; -.
DR   SMR; Q2FXF2; -.
DR   STRING; 1280.SAXN108_1807; -.
DR   EnsemblBacteria; ABD30960; ABD30960; SAOUHSC_01897.
DR   GeneID; 3920844; -.
DR   KEGG; sao:SAOUHSC_01897; -.
DR   PATRIC; fig|93061.5.peg.1726; -.
DR   eggNOG; COG1595; Bacteria.
DR   HOGENOM; CLU_047691_20_2_9; -.
DR   OMA; IILCENH; -.
DR   PHI-base; PHI:6112; -.
DR   PRO; PR:Q2FXF2; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..156
FT                   /note="RNA polymerase sigma factor SigS"
FT                   /id="PRO_0000367454"
FT   DNA_BIND        126..145
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           29..44
FT                   /note="Polymerase core binding"
FT   CONFLICT        22
FT                   /note="N -> D (in Ref. 1; AAN32728)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   156 AA;  19153 MW;  8285125CFA496E2B CRC64;
     MKFNDVYNKH QKIIHYLLKK YNISYNYDEY YQLLLIKMWQ LSQIYKPSSK QSLSSFLFTR
     LNFYLIDLFR QQNQLKDVIL CENNSPTLTE QPTYFNEHDL RLQDIFKLLN QRERLWLKLY
     LEGYKQFEIA EIMSLSLSTI KLIKMSVKRK CQHNFN