BH104_ARATH
ID BH104_ARATH Reviewed; 283 AA.
AC Q8L467; O23297;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transcription factor bHLH104;
DE AltName: Full=Basic helix-loop-helix protein 104;
DE Short=AtbHLH104;
DE Short=bHLH 104;
DE AltName: Full=Transcription factor EN 136;
DE AltName: Full=bHLH transcription factor bHLH104;
GN Name=BHLH104; Synonyms=EN136; OrderedLocusNames=At4g14410;
GN ORFNames=dl3245w, FCAALL.201;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [9]
RP INTERACTION WITH BTS.
RC STRAIN=cv. Columbia;
RX PubMed=20675571; DOI=10.1105/tpc.110.074096;
RA Long T.A., Tsukagoshi H., Busch W., Lahner B., Salt D.E., Benfey P.N.;
RT "The bHLH transcription factor POPEYE regulates response to iron deficiency
RT in Arabidopsis roots.";
RL Plant Cell 22:2219-2236(2010).
RN [10]
RP INTERACTION WITH BTS AND BHLH47/PYE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=25452667; DOI=10.1104/pp.114.250837;
RA Selote D., Samira R., Matthiadis A., Gillikin J.W., Long T.A.;
RT "Iron-binding E3 ligase mediates iron response in plants by targeting basic
RT helix-loop-helix transcription factors.";
RL Plant Physiol. 167:273-286(2015).
CC -!- SUBUNIT: Homodimer (Probable). Interacts with BTS and BHLH47/PYE
CC (PubMed:20675571, PubMed:25452667). {ECO:0000269|PubMed:20675571,
CC ECO:0000269|PubMed:25452667, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:25452667}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L467-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L467-2; Sequence=VSP_036096;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
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DR EMBL; AF488628; AAM10963.1; -; mRNA.
DR EMBL; Z97336; CAB10220.1; -; Genomic_DNA.
DR EMBL; AL161538; CAB78483.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83441.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83442.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66359.1; -; Genomic_DNA.
DR EMBL; AY102106; AAM26676.1; -; mRNA.
DR EMBL; AY133565; AAM91395.1; -; mRNA.
DR EMBL; AY088053; AAM65599.1; -; mRNA.
DR PIR; B71406; B71406.
DR RefSeq; NP_001328258.1; NM_001340935.1. [Q8L467-2]
DR RefSeq; NP_567431.1; NM_117520.4. [Q8L467-1]
DR RefSeq; NP_849383.1; NM_179052.2. [Q8L467-2]
DR AlphaFoldDB; Q8L467; -.
DR SMR; Q8L467; -.
DR BioGRID; 12383; 6.
DR IntAct; Q8L467; 2.
DR STRING; 3702.AT4G14410.1; -.
DR iPTMnet; Q8L467; -.
DR PaxDb; Q8L467; -.
DR PRIDE; Q8L467; -.
DR ProteomicsDB; 240691; -. [Q8L467-1]
DR EnsemblPlants; AT4G14410.1; AT4G14410.1; AT4G14410. [Q8L467-1]
DR EnsemblPlants; AT4G14410.2; AT4G14410.2; AT4G14410. [Q8L467-2]
DR EnsemblPlants; AT4G14410.3; AT4G14410.3; AT4G14410. [Q8L467-2]
DR GeneID; 827086; -.
DR Gramene; AT4G14410.1; AT4G14410.1; AT4G14410. [Q8L467-1]
DR Gramene; AT4G14410.2; AT4G14410.2; AT4G14410. [Q8L467-2]
DR Gramene; AT4G14410.3; AT4G14410.3; AT4G14410. [Q8L467-2]
DR KEGG; ath:AT4G14410; -.
DR Araport; AT4G14410; -.
DR TAIR; locus:2129710; AT4G14410.
DR eggNOG; ENOG502RXAH; Eukaryota.
DR HOGENOM; CLU_869720_0_0_1; -.
DR InParanoid; Q8L467; -.
DR OMA; ANKMAIF; -.
DR OrthoDB; 1293523at2759; -.
DR PhylomeDB; Q8L467; -.
DR PRO; PR:Q8L467; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8L467; baseline and differential.
DR Genevisible; Q8L467; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IGI:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR044818; ILR3-like.
DR PANTHER; PTHR46133; PTHR46133; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..283
FT /note="Transcription factor bHLH104"
FT /id="PRO_0000358792"
FT DOMAIN 130..181
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 96..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..124
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..18
FT /note="MYPSLDDDFVSDLFCFDQ -> MDVNLFGHDDSC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12679534"
FT /id="VSP_036096"
SQ SEQUENCE 283 AA; 31995 MW; AB758D25A07E12BB CRC64;
MYPSLDDDFV SDLFCFDQSN GAELDDYTQF GVNLQTDQED TFPDFVSYGV NLQQEPDEVF
SIGASQLDLS SYNGVLSLEP EQVGQQDCEV VQEEEVEINS GSSGGAVKEE QEHLDDDCSR
KRARTGSCSR GGGTKACRER LRREKLNERF MDLSSVLEPG RTPKTDKPAI LDDAIRILNQ
LRDEALKLEE TNQKLLEEIK SLKAEKNELR EEKLVLKADK EKTEQQLKSM TAPSSGFIPH
IPAAFNHNKM AVYPSYGYMP MWHYMPQSVR DTSRDQELRP PAA
