ID   SIGY_BACSU              Reviewed;         178 AA.
AC   P94370;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=RNA polymerase sigma factor SigY;
GN   Name=sigY; Synonyms=yxlB; OrderedLocusNames=BSU38700;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA   Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA   Fujita Y.;
RT   "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT   the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT   sacXY region.";
RL   Microbiology 142:3113-3123(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION AS A REGULATOR, INDUCTION, AND REGULATION BY YXLD AND YXLE.
RC   STRAIN=168 / CU1065;
RX   PubMed=12897008; DOI=10.1128/jb.185.16.4883-4890.2003;
RA   Cao M., Salzberg L., Tsai C.S., Mascher T., Bonilla C., Wang T., Ye R.W.,
RA   Marquez-Magana L., Helmann J.D.;
RT   "Regulation of the Bacillus subtilis extracytoplasmic function protein
RT   sigma(Y) and its target promoters.";
RL   J. Bacteriol. 185:4883-4890(2003).
RN   [4]
RP   INDUCTION BY NITROGEN STARVATION.
RC   STRAIN=168;
RX   PubMed=14769884; DOI=10.1093/jb/mvg225;
RA   Tojo S., Matsunaga M., Matsumoto T., Kang C.-M., Yamaguchi H., Asai K.,
RA   Sadaie Y., Yoshida K., Fujita Y.;
RT   "Organization and expression of the Bacillus subtilis sigY operon.";
RL   J. Biochem. 134:935-946(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Positively regulates the expression of the sigY-yxlCDEFG
CC       operon upon nitrogen starvation. Also positively regulates ybgB.
CC       {ECO:0000269|PubMed:12897008}.
CC   -!- INDUCTION: Positively autoregulated. Induced upon nitrogen starvation.
CC       {ECO:0000269|PubMed:12897008, ECO:0000269|PubMed:14769884}.
CC   -!- MISCELLANEOUS: Negatively regulated by both YxlD and YxlE.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D83026; BAA11733.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15896.1; -; Genomic_DNA.
DR   PIR; B69707; B69707.
DR   RefSeq; NP_391749.1; NC_000964.3.
DR   RefSeq; WP_003243678.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P94370; -.
DR   SMR; P94370; -.
DR   IntAct; P94370; 1.
DR   STRING; 224308.BSU38700; -.
DR   PaxDb; P94370; -.
DR   PRIDE; P94370; -.
DR   EnsemblBacteria; CAB15896; CAB15896; BSU_38700.
DR   GeneID; 937400; -.
DR   KEGG; bsu:BSU38700; -.
DR   PATRIC; fig|224308.179.peg.4189; -.
DR   eggNOG; COG1595; Bacteria.
DR   InParanoid; P94370; -.
DR   OMA; QAEVWRF; -.
DR   PhylomeDB; P94370; -.
DR   BioCyc; BSUB:BSU38700-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0006950; P:response to stress; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR039425; RNA_pol_sigma-70-like.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43133; PTHR43133; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS01063; SIGMA70_ECF; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..178
FT                   /note="RNA polymerase sigma factor SigY"
FT                   /id="PRO_0000094018"
FT   DNA_BIND        146..165
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           48..61
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   178 AA;  21358 MW;  2D1F42C3DDEABC6A CRC64;
     MDTQEEQRLI QQAKEGNDEA FTALFHYHYS FLYKYLLKLS LHPDLSEELV QETFLKGYIH
     LRSFQGRSKF STWLISIASR LYLDHQKKRK REWKRNQTVT EETIRKIKWD VSAKGAEWSE
     TLDLFSKLDP KLRTPVLLRH YYGYTYAEIG VMLQIKEGTV KSRVHKGLQQ IRKEWDDE