ID   SIL1_BOVIN              Reviewed;         462 AA.
AC   Q32KV6;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Nucleotide exchange factor SIL1;
DE   Flags: Precursor;
GN   Name=SIL1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for protein translocation and folding in the
CC       endoplasmic reticulum (ER). Functions as a nucleotide exchange factor
CC       for the ER lumenal chaperone HSPA5. {ECO:0000250|UniProtKB:Q9H173}.
CC   -!- SUBUNIT: Interacts with HSPA5. {ECO:0000250|UniProtKB:Q9H173}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000250|UniProtKB:Q9H173}.
CC   -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q9H173}.
CC   -!- PTM: Ubiquitinated by the CRL2(FEM1A) and CRL2(FEM1C) complexes, which
CC       recognize the -Lys-Xaa-Xaa-Arg C-degron at the C-terminus, leading to
CC       its degradation. {ECO:0000250|UniProtKB:Q9H173}.
CC   -!- SIMILARITY: Belongs to the SIL1 family. {ECO:0000305}.
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DR   EMBL; BC109907; AAI09908.1; -; mRNA.
DR   RefSeq; NP_001069276.1; NM_001075808.2.
DR   RefSeq; XP_005209496.1; XM_005209439.3.
DR   RefSeq; XP_005209498.1; XM_005209441.3.
DR   RefSeq; XP_005209499.1; XM_005209442.3.
DR   RefSeq; XP_010805655.1; XM_010807353.2.
DR   AlphaFoldDB; Q32KV6; -.
DR   SMR; Q32KV6; -.
DR   STRING; 9913.ENSBTAP00000045075; -.
DR   PaxDb; Q32KV6; -.
DR   PeptideAtlas; Q32KV6; -.
DR   PRIDE; Q32KV6; -.
DR   Ensembl; ENSBTAT00000047926; ENSBTAP00000045075; ENSBTAG00000033747.
DR   GeneID; 520650; -.
DR   KEGG; bta:520650; -.
DR   CTD; 64374; -.
DR   VEuPathDB; HostDB:ENSBTAG00000033747; -.
DR   VGNC; VGNC:34622; SIL1.
DR   eggNOG; KOG2160; Eukaryota.
DR   GeneTree; ENSGT00940000153909; -.
DR   HOGENOM; CLU_046547_1_0_1; -.
DR   InParanoid; Q32KV6; -.
DR   OMA; FQPTHEW; -.
DR   OrthoDB; 1501690at2759; -.
DR   TreeFam; TF324307; -.
DR   Proteomes; UP000009136; Chromosome 7.
DR   Bgee; ENSBTAG00000033747; Expressed in cumulus cell and 108 other tissues.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:Ensembl.
DR   GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Protein transport; Reference proteome;
KW   Signal; Translocation; Transport; Ubl conjugation.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..462
FT                   /note="Nucleotide exchange factor SIL1"
FT                   /id="PRO_0000223353"
FT   REGION          1..257
FT                   /note="Interaction with HSPA5 and localization to the
FT                   endoplasmic reticulum"
FT                   /evidence="ECO:0000250|UniProtKB:Q9EPK6"
FT   CARBOHYD        194
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        237
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   462 AA;  52581 MW;  475E782E8F1E8434 CRC64;
     MPHQGLLLFR MAPLCVLLSM LMVSCFNFCL CHKNSEFALT NPEKSSTKET ERKETEEELE
     PKILEVFHPT HEWQALRPGQ AVPAGSHVRL NLQTGAREVK LHDEDKFQTN LKGLKKGKRL
     DINTNTYTSQ DLKNALAKFK EGAEMENSKE DQERQAKVKR LFRPIEELKK DFEELNVVIE
     TDMQIMVRLI NKFNSSSSSL EEKIAALFDL EYYVHQMDNA QDLLSFGGLQ VVINGLNSTE
     PLVKEYAAFV LGAAFSSNPK VQVEAIEGGA LQKLLVILAT EQPLTTKKKA LFALCSLLRH
     FPYAQQQFLK LGGLQVLRSL VQEKGMEVLA VRVVTLLYDL VTEKMFAEED AELTWDMSPK
     RLQQYRQVHL LPSLQEQGWC EITAHLLALP EHDAREKVLQ TLGALLATCR DRFHQDTQLH
     RTLGTLQAEY QALAALELQE GEDEGYFREL LDSIDSLLTE LR