SIL1_DROME
ID SIL1_DROME Reviewed; 429 AA.
AC Q9VBV5;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Nucleotide exchange factor Sil1 {ECO:0000312|FlyBase:FBgn0039296};
DE Flags: Precursor;
GN Name=Sil1 {ECO:0000312|FlyBase:FBgn0039296};
GN ORFNames=CG10420 {ECO:0000312|FlyBase:FBgn0039296};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required for protein translocation and folding in the
CC endoplasmic reticulum (ER). Functions as a nucleotide exchange factor
CC for an ER lumenal chaperone of HSP70 family (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SIL1 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF56422.1; -; Genomic_DNA.
DR EMBL; AY058471; AAL13700.1; -; mRNA.
DR RefSeq; NP_651356.1; NM_143099.4.
DR AlphaFoldDB; Q9VBV5; -.
DR SMR; Q9VBV5; -.
DR BioGRID; 67953; 14.
DR IntAct; Q9VBV5; 1.
DR STRING; 7227.FBpp0084244; -.
DR GlyGen; Q9VBV5; 4 sites.
DR PaxDb; Q9VBV5; -.
DR PRIDE; Q9VBV5; -.
DR DNASU; 43034; -.
DR EnsemblMetazoa; FBtr0084870; FBpp0084244; FBgn0039296.
DR GeneID; 43034; -.
DR KEGG; dme:Dmel_CG10420; -.
DR UCSC; CG10420-RA; d. melanogaster.
DR CTD; 64374; -.
DR FlyBase; FBgn0039296; Sil1.
DR VEuPathDB; VectorBase:FBgn0039296; -.
DR eggNOG; KOG2160; Eukaryota.
DR GeneTree; ENSGT00940000153909; -.
DR HOGENOM; CLU_046547_1_0_1; -.
DR InParanoid; Q9VBV5; -.
DR OMA; NRFAHSQ; -.
DR OrthoDB; 1501690at2759; -.
DR PhylomeDB; Q9VBV5; -.
DR BioGRID-ORCS; 43034; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43034; -.
DR PRO; PR:Q9VBV5; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039296; Expressed in egg chamber and 24 other tissues.
DR Genevisible; Q9VBV5; DM.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; ISS:FlyBase.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; ISS:UniProtKB.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Endoplasmic reticulum; Glycoprotein; Protein transport;
KW Reference proteome; Signal; Translocation; Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..429
FT /note="Nucleotide exchange factor Sil1"
FT /id="PRO_0000223358"
FT REGION 70..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 104..135
FT /evidence="ECO:0000255"
FT MOTIF 426..429
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 150
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 199
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 400
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 429 AA; 47943 MW; 476349B0C0CD8EE4 CRC64;
MSGKQVVILL GSVLILGCLQ VAAATETDNK TNDFVATDEW QTIAEGQAIP RGLHVRINLQ
TGLKEAKLLD ESERGTSLQS QPDDQNARES HDDNEPLALD YKPDIIEESI RRVKEQKKSY
AELRKAYKEF QKNFRTDGEL IVQLIDQFRN FSRTPLESEM RSKLDCLENL EYLLHQIDNA
LMFIDNGGLD DVLLPIVVND TSTSLRVSAM RVLGSLASNN PKAQIKVFEK NFGSHLAQIL
TSSGNVGEIS AALHAFGALL RKFPLAQQRV LSTSGTQALI KVLQSPDVEL RSKAKVVTLI
SDLVLEKRSV LDVSKDDPEA SSTMAQYVLL DFESWLKTPG YCAAVDTVLT KEFLLLLEQP
EVVEQFATAL ETTEDMCTST WSQSSGLRHA LLTVRNRYAN STDEYRLEVS QILAKLCERL
FNKPKHTEL
