ID   SIL1_KLULA              Reviewed;         490 AA.
AC   Q6CKV0;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Nucleotide exchange factor SIL1;
DE   Flags: Precursor;
GN   Name=SIL1; OrderedLocusNames=KLLA0F07931g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for protein translocation and folding in the
CC       endoplasmic reticulum (ER). Functions as a nucleotide exchange factor
CC       for the ER lumenal chaperone KAR2 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with KAR2. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-
CC       ProRule:PRU10138}.
CC   -!- SIMILARITY: Belongs to the SIL1 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98147.1; -; Genomic_DNA.
DR   RefSeq; XP_455439.1; XM_455439.1.
DR   AlphaFoldDB; Q6CKV0; -.
DR   SMR; Q6CKV0; -.
DR   STRING; 28985.XP_455439.1; -.
DR   EnsemblFungi; CAG98147; CAG98147; KLLA0_F07931g.
DR   GeneID; 2895067; -.
DR   KEGG; kla:KLLA0_F07931g; -.
DR   eggNOG; KOG2160; Eukaryota.
DR   HOGENOM; CLU_034955_0_0_1; -.
DR   InParanoid; Q6CKV0; -.
DR   OMA; GLDIRMN; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR031884; Sil1_fungi.
DR   Pfam; PF16782; SIL1; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Glycoprotein; Protein transport; Reference proteome;
KW   Signal; Translocation; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..490
FT                   /note="Nucleotide exchange factor SIL1"
FT                   /id="PRO_0000223363"
FT   MOTIF           487..490
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT   CARBOHYD        258
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   490 AA;  55892 MW;  3DB5612E46C4FC75 CRC64;
     MRVKCVNRAI YVLTVLLFSR LVVSQVVLTP SNSNADPKQK DTANTVAAVE ANNDANIAKK
     DAESDLVIGD HLVCNTKECY PIGFVPSTEW KEIRPGQRLP PGLDIRVSLE KGVREAKLPE
     PGSENIGNEE EDVKGLVLGA EGSTLSESEL KETSEDLENE QSGFKLNNAE KESDILQQET
     DLKIAVSDNA EATSNEPAGH EFSEDFAKIK SLMQSPDEKT WEEVETLLDD LVEFAHDYKK
     GFKILSNEFE LLEYLSFNDT LSIQIRELAA RIIVSSLRNN PPSIDFVNEK YPQTTFKLCE
     HLSELQASQG SKLLIKRFLS ILDVLLSRTE YVSIKDDVLW RLYQIEDPSS KIKILEIIAK
     FYNEKNEQVI DTVQQDMKTV QKWVNELTTI IQTPELDELH LRSFFHCISF IKTRFKNRVK
     IDSDFLNWLI DEIEVRNEKS KDDIYKRDVD QLEFDNQLAK SRHAVFGNPN AARLKERLFD
     DDDTLIADEL