SILS_SALTM
ID SILS_SALTM Reviewed; 497 AA.
AC Q9ZHD4;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Probable sensor kinase SilS;
DE EC=2.7.13.3;
GN Name=silS;
OS Salmonella typhimurium.
OG Plasmid pMG101.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=90371;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9930866; DOI=10.1038/5545;
RA Gupta A., Matsui K., Lo J.-F., Silver S.;
RT "Molecular basis for resistance to silver cations in Salmonella.";
RL Nat. Med. 5:183-188(1999).
CC -!- FUNCTION: Component of the sil cation-efflux system that confers
CC resistance to silver. Probable member of a two-component regulatory
CC system SilS/SilR. May activate SilR by phosphorylation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
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DR EMBL; AF067954; AAD11744.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9ZHD4; -.
DR SMR; Q9ZHD4; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR006290; CztS_silS_copS.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR01386; cztS_silS_copS; 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Plasmid; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..497
FT /note="Probable sensor kinase SilS"
FT /id="PRO_0000074878"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..186
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..497
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 208..261
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 269..487
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 272
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ SEQUENCE 497 AA; 56247 MW; C5F19D6DC11A0D96 CRC64;
MHSKPSRLPF SLALRLTFFI SLSTILAFIA FTWFMLHSVE KHFAEQDVSD LQQISTTLSR
ILQSPADPDE KKVSKIKESI ASYRNVALLL LNPRGEVLYS SAQGAALRPA VNSADFSEHS
RARDVFLWTV EDTARAMDTG SGMKMETYRI IASSGQATFQ GKQQNYVMLT GLSINFHLHY
LDALKKNLIA IAVVISLLIV LIIRIAVRQG HLPLRNVSNA IKNITSENLD ARLEPTRVPI
ELEQLVISFN HMIGKIEDVF TRQANFSADI AHEIRTPITN LVTQTEIALS QDRTQKELED
VLYSSLEEYN RMTKMVSDML FLAQADNNQL IPDRVRFDLQ SQNSLKVFRV FSEALGPKET
PILLLKFNGM PCLVEGDPQM FRRAINNLLS NALRYTPEGQ AITVSIREQE SFFDLVIENP
GKPIPEEHLS RLFDRFYRVD PSRQRKGEGS GIGLAIVKSI VEAHHGRVQV ESDVHSTRFI
LSVPRLEKMI PDTQCWE
