SIM1_MOUSE
ID SIM1_MOUSE Reviewed; 765 AA.
AC Q61045; O70284; P70183;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 3.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Single-minded homolog 1;
DE Short=mSIM1;
GN Name=Sim1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=129/Sv, and Swiss Webster;
RC TISSUE=Embryonic brain, and Embryonic stem cell;
RX PubMed=8812055; DOI=10.1006/mcne.1996.0001;
RA Fan C.-M., Kuwana E., Bulfone A., Fletcher C.F., Copeland N.G.,
RA Jenkins N.A., Crews S., Martinez S., Puelles L., Rubenstein J.L.,
RA Tessier-Lavigne M.;
RT "Expression patterns of two murine homologs of Drosophila single-minded
RT suggest possible roles in embryonic patterning and in the pathogenesis of
RT Down syndrome.";
RL Mol. Cell. Neurosci. 7:1-16(1996).
RN [2]
RP ERRATUM OF PUBMED:8812055.
RX PubMed=8875433; DOI=10.1006/mcne.1996.0037;
RA Fan C.-M., Kuwana E., Bulfone A., Fletcher C.F., Copeland N.G.,
RA Jenkins N.A., Crews S., Martinez S., Puelles L., Rubenstein J.L.,
RA Tessier-Lavigne M.;
RL Mol. Cell. Neurosci. 7:519-519(1996).
RN [3]
RP SEQUENCE REVISION TO C-TERMINUS.
RX PubMed=9199934; DOI=10.1101/gr.7.6.615;
RA Chrast R., Scott H.S., Chen H., Kudoh J., Rossier C., Minoshima S.,
RA Wang Y., Shimizu N., Antonarakis S.E.;
RT "Cloning of two human homologs of the Drosophila single-minded gene SIM1 on
RT chromosome 6q and SIM2 on 21q within the Down syndrome chromosomal
RT region.";
RL Genome Res. 7:615-624(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=8927054; DOI=10.1128/mcb.16.10.5865;
RA Ema M., Morita M., Ikawa S., Tanaka M., Matsuda Y., Gotoh O., Saijoh Y.,
RA Fujii H., Hamada H., Kikuchi Y., Fujii-Kuriyama Y.;
RT "Two new members of the murine Sim gene family are transcriptional
RT repressors and show different expression patterns during mouse
RT embryogenesis.";
RL Mol. Cell. Biol. 16:5865-5875(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RA Hosoya T.;
RT "Mouse single-minded1 (mSim1) gene.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBUNIT.
RX PubMed=9020169; DOI=10.1074/jbc.272.7.4451;
RA Probst M.R., Fan C.-M., Tessier-Lavigne M., Hankinson O.;
RT "Two murine homologs of the Drosophila single-minded protein that interact
RT with the mouse aryl hydrocarbon receptor nuclear translocator protein.";
RL J. Biol. Chem. 272:4451-4457(1997).
RN [7]
RP INTERACTION WITH ARNT AND ARNT2.
RX PubMed=27782878; DOI=10.7554/elife.18790;
RA Wu D., Su X., Potluri N., Kim Y., Rastinejad F.;
RT "NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family
RT as multi-ligand binding transcription factors.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Transcriptional factor that may have pleiotropic effects
CC during embryogenesis and in the adult.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Heterodimer; forms a heterodimer with ARNT, ARNT2.
CC {ECO:0000269|PubMed:27782878, ECO:0000269|PubMed:9020169}.
CC -!- INTERACTION:
CC Q61045; P53762: Arnt; NbExp=4; IntAct=EBI-78890, EBI-78852;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00632,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Detected in lung, skeletal muscle and kidney.
CC During fetal development it is found in the CNS, developing kidney,
CC mesodermal and endodermal tissues, including developing somites,
CC mesonephric duct, and foregut.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA91201.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; U40575; AAA91201.1; ALT_SEQ; mRNA.
DR EMBL; AF038857; AAC05481.1; -; Genomic_DNA.
DR EMBL; AF038853; AAC05481.1; JOINED; Genomic_DNA.
DR EMBL; AF038854; AAC05481.1; JOINED; Genomic_DNA.
DR EMBL; AF038856; AAC05481.1; JOINED; Genomic_DNA.
DR EMBL; AF044913; AAC05481.1; JOINED; Genomic_DNA.
DR EMBL; AF038855; AAC05481.1; JOINED; Genomic_DNA.
DR EMBL; D79209; BAA11467.1; -; mRNA.
DR EMBL; AB013491; BAA28270.1; -; Genomic_DNA.
DR CCDS; CCDS48556.1; -.
DR RefSeq; NP_035506.2; NM_011376.3.
DR RefSeq; XP_006512690.1; XM_006512627.3.
DR AlphaFoldDB; Q61045; -.
DR SMR; Q61045; -.
DR BioGRID; 203254; 2.
DR CORUM; Q61045; -.
DR IntAct; Q61045; 3.
DR MINT; Q61045; -.
DR STRING; 10090.ENSMUSP00000020071; -.
DR iPTMnet; Q61045; -.
DR PhosphoSitePlus; Q61045; -.
DR PaxDb; Q61045; -.
DR PRIDE; Q61045; -.
DR ProteomicsDB; 257250; -.
DR Antibodypedia; 899; 162 antibodies from 25 providers.
DR DNASU; 20464; -.
DR Ensembl; ENSMUST00000020071; ENSMUSP00000020071; ENSMUSG00000019913.
DR GeneID; 20464; -.
DR KEGG; mmu:20464; -.
DR UCSC; uc007fal.1; mouse.
DR CTD; 6492; -.
DR MGI; MGI:98306; Sim1.
DR VEuPathDB; HostDB:ENSMUSG00000019913; -.
DR eggNOG; KOG3559; Eukaryota.
DR GeneTree; ENSGT00940000156143; -.
DR HOGENOM; CLU_010044_4_0_1; -.
DR InParanoid; Q61045; -.
DR OMA; ANTSPCE; -.
DR OrthoDB; 231698at2759; -.
DR PhylomeDB; Q61045; -.
DR TreeFam; TF317772; -.
DR BioGRID-ORCS; 20464; 7 hits in 74 CRISPR screens.
DR ChiTaRS; Sim1; mouse.
DR PRO; PR:Q61045; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q61045; protein.
DR Bgee; ENSMUSG00000019913; Expressed in diencephalon lateral wall and 114 other tissues.
DR ExpressionAtlas; Q61045; baseline and differential.
DR Genevisible; Q61045; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0001657; P:ureteric bud development; IEP:UniProtKB.
DR CDD; cd00130; PAS; 2.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR InterPro; IPR013655; PAS_fold_3.
DR InterPro; IPR010578; SIM_C.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF00989; PAS; 1.
DR Pfam; PF08447; PAS_3; 1.
DR Pfam; PF06621; SIM_C; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00086; PAC; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 2.
DR PROSITE; PS51302; SIM_C; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..765
FT /note="Single-minded homolog 1"
FT /id="PRO_0000127440"
FT DOMAIN 1..53
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 77..147
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 218..288
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 292..335
FT /note="PAC"
FT DOMAIN 336..765
FT /note="Single-minded C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00632"
FT REGION 352..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 527..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 368..387
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 352..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 133
FT /note="H -> L (in Ref. 1; AAA91201/AAC05481)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="Missing (in Ref. 1; AAA91201)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="P -> R (in Ref. 1; AAA91201/AAC05481)"
FT /evidence="ECO:0000305"
FT CONFLICT 480
FT /note="A -> P (in Ref. 1; AAA91201)"
FT /evidence="ECO:0000305"
FT CONFLICT 537
FT /note="D -> S (in Ref. 1; AAA91201)"
FT /evidence="ECO:0000305"
FT CONFLICT 698
FT /note="F -> K (in Ref. 1; AAA91201)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 765 AA; 85541 MW; B1A7F7DA8578CD17 CRC64;
MKEKSKNAAR TRREKENSEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRVVFPEGL
GEAWGHTSRT SPLDNVGREL GSHLLQTLDG FIFVVAPDGK IMYISETASV HLGLSQVELT
GNSIYEYIHP ADHDEMTAVL TAHQPYHSHF VQEYEIERSF FLRMKCVLAK RNAGLTCGGY
KVIHCSGYLK IRQYSLDMSP FDGCYQNVGL VAVGHSLPPS AVTEIKLHSN MFMFRASLDM
KLIFLDSRVA ELTGYEPQDL IEKTLYHHVH GCDTFHLRCA HHLLLVKGQV TTKYYRFLAK
QGGWVWVQSY ATIVHNSRSS RPHCIVSVNY VLTDTEYKGL QLSLDQISAS KPTFSYTSSS
TPTISDNRKG AKSRLSSSKS KSRTSPYPQY SGFHTERSES DHDSQWGGSP LTDTASPQLL
DPERPGSQHE LSCAYRQFPD RSSLCYGFAL DHSRLVEDRH FHTQACEGGR CEAGRYFLGA
PPTGRDPWWG SRAALPLTKA SPESREAYEN SMPHITSIHR IHGRGHWDED SVVSSPDPGS
ASESGDRYRT EQYQNSPHEP SKIETLIRAT QQMIKEEENR LQLRKAPPDQ LASINGAGKK
HSLCFANYQQ PPPTGEVCHS SALASTSPCD HIQQREGKML SPHENDYDNS PTALSRISSP
SSDRITKSSL ILAKDYLHSD MSPHQTAGDH PAISPNCFGS HRQYFDKHAY TLTGYALEHL
YDSETIRNYS LGCNGSHFDV TSHLRMQPDP AQGHKGTSVI ITNGS
