SIM20_BOVIN
ID SIM20_BOVIN Reviewed; 67 AA.
AC F1MDB2;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Small integral membrane protein 20;
DE AltName: Full=Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa {ECO:0000250|UniProtKB:Q8N5G0};
DE Short=MITRAC7 {ECO:0000250|UniProtKB:Q8N5G0};
DE Contains:
DE RecName: Full=Phoenixin-14 {ECO:0000303|PubMed:22963497};
DE Short=PNX-14 {ECO:0000303|PubMed:22963497};
DE Contains:
DE RecName: Full=Phoenixin-20 {ECO:0000250|UniProtKB:C0HLM6};
DE Short=PNX-20 {ECO:0000250|UniProtKB:C0HLM6};
GN Name=SMIM20 {ECO:0000312|Ensembl:ENSBTAP00000039462};
GN Synonyms=LOC101904614 {ECO:0000312|Ensembl:ENSBTAP00000056059};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN [1] {ECO:0000312|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000312|Proteomes:UP000009136};
RX PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT "A whole-genome assembly of the domestic cow, Bos taurus.";
RL Genome Biol. 10:R42.01-R42.10(2009).
RN [2] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, PROTEIN SEQUENCE OF 51-64, FUNCTION,
RP TISSUE SPECIFICITY, AND AMIDATION AT PHE-64.
RX PubMed=22963497; DOI=10.1111/j.1365-2826.2012.02381.x;
RA Yosten G.L., Lyu R.M., Hsueh A.J., Avsian-Kretchmer O., Chang J.K.,
RA Tullock C.W., Dun S.L., Dun N., Samson W.K.;
RT "A novel reproductive peptide, phoenixin.";
RL J. Neuroendocrinol. 25:206-215(2013).
CC -!- FUNCTION: [Small integral membrane protein 20]: Component of the MITRAC
CC (mitochondrial translation regulation assembly intermediate of
CC cytochrome c oxidase complex) complex, that regulates cytochrome c
CC oxidase assembly (By similarity). Promotes the progression of complex
CC assembly after the association of MT-CO1/COX1 with COX4I1 and COX6C (By
CC similarity). Chaperone-like assembly factor required to stabilize newly
CC synthesized MT-CO1/COX1 and to prevent its premature turnover (By
CC similarity). {ECO:0000250|UniProtKB:Q8N5G0}.
CC -!- FUNCTION: [Phoenixin-14]: Peptide involved in a broad spectrum of
CC regulatory functions (By similarity). Is a ligand for GPR173 (By
CC similarity). As part of the reproductive cycle, it regulates
CC gonadotropin-releasing hormone (GnRH) signaling in the hypothalamus and
CC pituitary gland which augments the release of luteinizing hormone (By
CC similarity). Plays a protective role in memory retention through
CC activation of GNRHR (By similarity). Regulates the secretion of AVP by
CC hypothalamic neurons (By similarity). Plays a role in the transduction
CC of the itch sensation (By similarity). Induces anxiolytic effects,
CC reducing behavior associated with anxiety (By similarity). Regulates
CC food intake as well as satiation and satiety (By similarity). In the
CC ovary, it regulates follicular growth by stimulating granulosa cell
CC proliferation by increasing the expression of GPR173, CREB1, CYP19A1,
CC KITLG, FSHR, and LHCGR (By similarity). It also increases the
CC production of estradiol (E2) (By similarity). In the heart, it
CC regulates contractility and relaxation (By similarity). It also plays a
CC cardioprotective role during ischemia, where it activates the SAFE and
CC RISK pathways (By similarity). Stimulates the proliferation and
CC differentiation of preadipocytes (By similarity). In pancreatic islet
CC cells, it induces proliferation of islet cells as well as the
CC production of INS (By similarity). {ECO:0000250|UniProtKB:C0HLM6,
CC ECO:0000250|UniProtKB:D3Z7Q2, ECO:0000250|UniProtKB:Q8N5G0}.
CC -!- FUNCTION: [Phoenixin-20]: Peptide involved in a broad spectrum of
CC regulatory functions (By similarity). Is a ligand for GPR173 (By
CC similarity). As part of the reproductive cycle, it regulates
CC gonadotropin-releasing hormone (GnRH) signaling in the hypothalamus and
CC pituitary gland which augments the release of luteinizing hormone (By
CC similarity). Plays a protective role in memory retention through
CC activation of GNRHR (By similarity). Regulates the secretion of AVP by
CC hypothalamic neurons (By similarity). Plays a role in the transduction
CC of the itch sensation (By similarity). Induces anxiolytic effects,
CC reducing behavior associated with anxiety (By similarity). Regulates
CC food intake as well as satiation and satiety (By similarity). In the
CC ovary, it regulates follicular growth by stimulating granulosa cell
CC proliferation by increasing the expression of GPR173, CREB1, CYP19A1,
CC KITLG, FSHR, and LHCGR (By similarity). It also increases the
CC production of estradiol (E2) (By similarity). In the heart, it
CC regulates contractility and relaxation (By similarity). It also plays a
CC cardioprotective role during ischemia, where it activates the SAFE and
CC RISK pathways (By similarity). Stimulates the proliferation and
CC differentiation of preadipocytes (By similarity). In pancreatic islet
CC cells, it induces proliferation of islet cells as well as the
CC production of INS (By similarity). {ECO:0000250|UniProtKB:C0HLM6,
CC ECO:0000250|UniProtKB:D3Z7Q2, ECO:0000250|UniProtKB:Q8N5G0}.
CC -!- SUBUNIT: Component of the MITRAC (mitochondrial translation regulation
CC assembly intermediate of cytochrome c oxidase complex) complex, the
CC core components of this complex being COA3/MITRAC12 and COX14 (By
CC similarity). Interacts with COA3/MITRAC12 and COX4I1 (By similarity).
CC Directly interacts with newly synthesized MT-CO1/COX1 (By similarity).
CC {ECO:0000250|UniProtKB:Q8N5G0}.
CC -!- SUBCELLULAR LOCATION: [Small integral membrane protein 20]:
CC Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q8N5G0}; Single-
CC pass membrane protein {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Phoenixin-14]: Secreted
CC {ECO:0000250|UniProtKB:C0HLM6}.
CC -!- SUBCELLULAR LOCATION: [Phoenixin-20]: Secreted
CC {ECO:0000250|UniProtKB:C0HLM6}.
CC -!- TISSUE SPECIFICITY: Expressed in the heart (at protein level).
CC {ECO:0000269|PubMed:22963497}.
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DR RefSeq; NP_001138900.1; NM_001145428.1.
DR RefSeq; XP_005217461.1; XM_005217404.2.
DR RefSeq; XP_015322448.1; XM_015466962.1.
DR AlphaFoldDB; F1MDB2; -.
DR SMR; F1MDB2; -.
DR STRING; 9913.ENSBTAP00000039462; -.
DR PaxDb; F1MDB2; -.
DR PRIDE; F1MDB2; -.
DR Ensembl; ENSBTAT00000039673; ENSBTAP00000039462; ENSBTAG00000003897.
DR Ensembl; ENSBTAT00000063958; ENSBTAP00000056059; ENSBTAG00000045729.
DR GeneID; 101904614; -.
DR GeneID; 615093; -.
DR KEGG; bta:101904614; -.
DR KEGG; bta:615093; -.
DR CTD; 389203; -.
DR VEuPathDB; HostDB:ENSBTAG00000003897; -.
DR VEuPathDB; HostDB:ENSBTAG00000045729; -.
DR eggNOG; ENOG502S83Z; Eukaryota.
DR GeneTree; ENSGT00390000002398; -.
DR HOGENOM; CLU_175940_1_0_1; -.
DR InParanoid; F1MDB2; -.
DR OMA; VYPLTHK; -.
DR OrthoDB; 1639960at2759; -.
DR TreeFam; TF353700; -.
DR Proteomes; UP000009136; Chromosome 16.
DR Proteomes; UP000009136; Chromosome 6.
DR Bgee; ENSBTAG00000003897; Expressed in rumen papilla and 106 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IBA:GO_Central.
DR InterPro; IPR027917; SMIM20.
DR PANTHER; PTHR34923; PTHR34923; 1.
DR Pfam; PF15061; DUF4538; 1.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Secreted; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..67
FT /note="Small integral membrane protein 20"
FT /id="PRO_0000449027"
FT PEPTIDE 45..64
FT /note="Phoenixin-20"
FT /id="PRO_0000449028"
FT PEPTIDE 51..64
FT /note="Phoenixin-14"
FT /evidence="ECO:0000269|PubMed:22963497"
FT /id="PRO_0000449029"
FT TOPO_DOM 1..6
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000305"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..67
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000305"
FT MOD_RES 64
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:22963497"
SQ SEQUENCE 67 AA; 7702 MW; FCBA42B976E900AA CRC64;
MSRNLRTALI FGGFISLIGA AFYPIYFRPL MRLEEYKKEQ AINRAGIVQE DVQPPGLKVW
SDPFGRK
