BH121_ARATH
ID BH121_ARATH Reviewed; 337 AA.
AC Q9LT23; Q8VZA6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Transcription factor bHLH121;
DE AltName: Full=Basic helix-loop-helix protein 121;
DE Short=AtbHLH121;
DE Short=bHLH 121;
DE AltName: Full=Transcription factor EN 138;
DE AltName: Full=bHLH transcription factor bHLH121;
GN Name=BHLH121; Synonyms=EN138; OrderedLocusNames=At3g19860;
GN ORFNames=MPN9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, GENE FAMILY,
RP AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q9LT23-2; Q5XVH0: BHLH109; NbExp=3; IntAct=EBI-15197803, EBI-15193531;
CC Q9LT23-2; Q9LXU1: PIM1; NbExp=3; IntAct=EBI-15197803, EBI-15193025;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LT23-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LT23-2; Sequence=VSP_036103;
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
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DR EMBL; AF488633; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AB025631; BAB01300.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76300.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76301.1; -; Genomic_DNA.
DR EMBL; AY065107; AAL38283.1; -; mRNA.
DR EMBL; AY081703; AAM10265.1; -; mRNA.
DR RefSeq; NP_001030729.1; NM_001035652.4. [Q9LT23-1]
DR RefSeq; NP_188620.1; NM_112876.6. [Q9LT23-2]
DR AlphaFoldDB; Q9LT23; -.
DR SMR; Q9LT23; -.
DR BioGRID; 6855; 17.
DR IntAct; Q9LT23; 16.
DR STRING; 3702.AT3G19860.2; -.
DR PaxDb; Q9LT23; -.
DR PRIDE; Q9LT23; -.
DR EnsemblPlants; AT3G19860.1; AT3G19860.1; AT3G19860. [Q9LT23-2]
DR EnsemblPlants; AT3G19860.2; AT3G19860.2; AT3G19860. [Q9LT23-1]
DR GeneID; 821523; -.
DR Gramene; AT3G19860.1; AT3G19860.1; AT3G19860. [Q9LT23-2]
DR Gramene; AT3G19860.2; AT3G19860.2; AT3G19860. [Q9LT23-1]
DR KEGG; ath:AT3G19860; -.
DR Araport; AT3G19860; -.
DR TAIR; locus:2092216; AT3G19860.
DR eggNOG; ENOG502QRHF; Eukaryota.
DR InParanoid; Q9LT23; -.
DR PhylomeDB; Q9LT23; -.
DR PRO; PR:Q9LT23; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LT23; baseline and differential.
DR Genevisible; Q9LT23; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR044579; bHLH11/121.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR47001; PTHR47001; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..337
FT /note="Transcription factor bHLH121"
FT /id="PRO_0000358806"
FT DOMAIN 58..108
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 235..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..267
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..53
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12679534,
FT ECO:0000303|PubMed:14593172"
FT /id="VSP_036103"
FT CONFLICT 299
FT /note="N -> D (in Ref. 1; AF488633)"
FT /evidence="ECO:0000305"
FT CONFLICT 303
FT /note="I -> M (in Ref. 1; AF488633)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="S -> P (in Ref. 1; AF488633)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 337 AA; 37920 MW; 7BCC04175438E9F3 CRC64;
MGIRENGIML VSRERERARR LENRESIFAE PPCLLLAHRI SPSPSILPAE EEVMDVSARK
SQKAGREKLR REKLNEHFVE LGNVLDPERP KNDKATILTD TVQLLKELTS EVNKLKSEYT
ALTDESRELT QEKNDLREEK TSLKSDIENL NLQYQQRLRS MSPWGAAMDH TVMMAPPPSF
PYPMPIAMPP GSIPMHPSMP SYTYFGNQNP SMIPAPCPTY MPYMPPNTVV EQQSVHIPQN
PGNRSREPRA KVSRESRSEK AEDSNEVATQ LELKTPGSTS DKDTLQRPEK TKRCKRNNNN
NSIEESSHSS KCSSSPSVRD HSSSSSVAGG QKPDDAK
