SIMC1_MOUSE
ID SIMC1_MOUSE Reviewed; 1354 AA.
AC E9Q6E9; Q3TM32; Q8C747; Q8CE66;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=SUMO-interacting motif-containing protein 1;
DE AltName: Full=Platform element for inhibition of autolytic degradation {ECO:0000303|PubMed:23707407};
GN Name=Simc1; Synonyms=Pleiad {ECO:0000303|PubMed:23707407};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 713-1354 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Mammary gland, Skin, and Stomach;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP IDENTIFICATION, ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RX PubMed=23707407; DOI=10.1016/j.jmb.2013.05.009;
RA Ono Y., Iemura S., Novak S.M., Doi N., Kitamura F., Natsume T.,
RA Gregorio C.C., Sorimachi H.;
RT "PLEIAD/SIMC1/C5orf25, a novel autolysis regulator for a skeletal-muscle-
RT specific calpain, CAPN3, scaffolds a CAPN3 substrate, CTBP1.";
RL J. Mol. Biol. 425:2955-2972(2013).
CC -!- FUNCTION: Inhibits the protease activity of CAPN3.
CC {ECO:0000250|UniProtKB:Q8NDZ2}.
CC -!- SUBUNIT: Interacts (via SIM domains) with SUMO1 and SUMO2. Interacts
CC with CAPN3 and CTBP1. {ECO:0000250|UniProtKB:Q8NDZ2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms may exist. {ECO:0000305|PubMed:23707407};
CC Name=1; Synonyms=PLEIADa {ECO:0000303|PubMed:23707407};
CC IsoId=E9Q6E9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=E9Q6E9-2; Sequence=VSP_060887, VSP_060888;
CC -!- TISSUE SPECIFICITY: Skeletal muscle. {ECO:0000269|PubMed:23707407}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC35039.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE38610.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK052561; BAC35039.1; ALT_INIT; mRNA.
DR EMBL; AK028926; BAC26197.1; -; mRNA.
DR EMBL; AK166176; BAE38610.1; ALT_INIT; mRNA.
DR EMBL; AC155262; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC165145; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS26528.2; -. [E9Q6E9-1]
DR RefSeq; NP_795961.3; NM_176987.4. [E9Q6E9-1]
DR AlphaFoldDB; E9Q6E9; -.
DR STRING; 10090.ENSMUSP00000113676; -.
DR iPTMnet; E9Q6E9; -.
DR PhosphoSitePlus; E9Q6E9; -.
DR EPD; E9Q6E9; -.
DR MaxQB; E9Q6E9; -.
DR PaxDb; E9Q6E9; -.
DR PRIDE; E9Q6E9; -.
DR ProteomicsDB; 351391; -. [E9Q6E9-1]
DR Antibodypedia; 60845; 52 antibodies from 12 providers.
DR DNASU; 319719; -.
DR Ensembl; ENSMUST00000121401; ENSMUSP00000113676; ENSMUSG00000043183. [E9Q6E9-1]
DR GeneID; 319719; -.
DR KEGG; mmu:319719; -.
DR UCSC; uc007qoe.2; mouse. [E9Q6E9-1]
DR CTD; 375484; -.
DR MGI; MGI:2442599; Simc1.
DR VEuPathDB; HostDB:ENSMUSG00000043183; -.
DR eggNOG; ENOG502RR6K; Eukaryota.
DR GeneTree; ENSGT00940000153451; -.
DR HOGENOM; CLU_264356_0_0_1; -.
DR InParanoid; E9Q6E9; -.
DR OMA; GDAIQSP; -.
DR OrthoDB; 117288at2759; -.
DR TreeFam; TF332523; -.
DR BioGRID-ORCS; 319719; 1 hit in 58 CRISPR screens.
DR ChiTaRS; Simc1; mouse.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; E9Q6E9; protein.
DR Bgee; ENSMUSG00000043183; Expressed in undifferentiated genital tubercle and 248 other tissues.
DR ExpressionAtlas; E9Q6E9; baseline and differential.
DR Genevisible; E9Q6E9; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0030017; C:sarcomere; ISS:UniProtKB.
DR GO; GO:0030414; F:peptidase inhibitor activity; ISS:UniProtKB.
DR GO; GO:0032184; F:SUMO polymer binding; ISO:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Alternative splicing; Protease inhibitor; Reference proteome.
FT CHAIN 1..1354
FT /note="SUMO-interacting motif-containing protein 1"
FT /id="PRO_0000451959"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 432..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..770
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 783..870
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 945..1354
FT /note="Required for inhibition of CAPN3 protease activity"
FT /evidence="ECO:0000250|UniProtKB:Q8NDZ2"
FT REGION 1111..1142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 45..49
FT /note="SUMO interaction motif 1 (SIM); mediates the binding
FT to polysumoylated substrates"
FT /evidence="ECO:0000250|UniProtKB:Q8NDZ2"
FT MOTIF 64..68
FT /note="SUMO interaction motif 2 (SIM); mediates the binding
FT to polysumoylated substrates"
FT /evidence="ECO:0000250|UniProtKB:Q8NDZ2"
FT COMPBIAS 432..461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 530..545
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 561..580
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 591..614
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 627..642
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 654..697
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 712..726
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..758
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 787..824
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 841..869
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1112..1142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..858
FT /note="MEDFIVISDDSGSESSAGTRSGRARRLRRALSRTPGALPRRTVDFIDLTREN
FT RARTKDRNGLCVIDLTRNEEENRPIATLDLTLEPVASSQKEPTSLQTCTSLSGKEVMEA
FT HEDRGSQPAAQRIINNDPVDLDLLEENLFEGSRPPTSISQDSVYPPEPNCSSITYKGDL
FT SFLTSLQLSSDVSSFSSTSNQRASLPCPQQDVPCQSQGLLCPLQALSCPQTASPCPPRA
FT SSCPPQALSCPPQALSCPSQTLQCQLQALPQPPQEVPCSTQNVPCPQQNMPSTPQGLSW
FT HPRHTLYPYQDTLGLPQDVPGRPQNMSYPQDVTQLQDMPWSLHDMPLSLQDVLQSLQDV
FT PPLLGDVPPSPEVMQLPGYVTQTSRDVIQSPAGVTQSLGSMMQSPGSVTQSLRSVMQSS
FT GSVTQSLRSVMQSSGSVTQSLRSVMQSSGSVMQSPGGVTQSLRSVTQSPGGVMQSPGGV
FT MQSPRDVMQSPRDVMQSPRDVMQSPRGVTQSLGSMMQSPGGVMQSLRSVMQSSGGVTQS
FT LRSVMQSPGGVMQSPGGVTQSPRGMIKSPGMMLSPGDVIQSLNSVPQSSRDRMQSAGHV
FT PSASGDAIQSPGGMSPTSRDRMQSPGGVSLATEDSIQLPGGVPLSSDVIQSQGGVPLSS
FT KDRMQSPGGVPPSSGDMIQSQGGVPQSLGDAIQSAGGVPQSSGDAIQSPGGVSLATGDS
FT IQLPGGVPLSSGDVIQSQGGVPRSSRDRMQSPGGVPPSSGGVIQSPGGVSPASGDAIQS
FT PGSVLPASGDAIQSAGGVLLASGDAIQSPRSVPQSPSGTLRSPGNMSESLGDTPNLSGD
FT VSNSPQALLDLARDRPKSSPNDVQNRDTPMDISASSSSS -> MQYSHQEVCYRHQEMQ
FT YSQQEVCYWHQEMQ (in isoform 2)"
FT /id="VSP_060887"
FT VAR_SEQ 1022
FT /note="Q -> QYEPLQ (in isoform 2)"
FT /id="VSP_060888"
FT CONFLICT 814
FT /note="P -> L (in Ref. 1; BAE38610)"
FT /evidence="ECO:0000305"
FT CONFLICT 887
FT /note="R -> K (in Ref. 1; BAE38610)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1354 AA; 146618 MW; D9B2573C7AA23360 CRC64;
MEDFIVISDD SGSESSAGTR SGRARRLRRA LSRTPGALPR RTVDFIDLTR ENRARTKDRN
GLCVIDLTRN EEENRPIATL DLTLEPVASS QKEPTSLQTC TSLSGKEVME AHEDRGSQPA
AQRIINNDPV DLDLLEENLF EGSRPPTSIS QDSVYPPEPN CSSITYKGDL SFLTSLQLSS
DVSSFSSTSN QRASLPCPQQ DVPCQSQGLL CPLQALSCPQ TASPCPPRAS SCPPQALSCP
PQALSCPSQT LQCQLQALPQ PPQEVPCSTQ NVPCPQQNMP STPQGLSWHP RHTLYPYQDT
LGLPQDVPGR PQNMSYPQDV TQLQDMPWSL HDMPLSLQDV LQSLQDVPPL LGDVPPSPEV
MQLPGYVTQT SRDVIQSPAG VTQSLGSMMQ SPGSVTQSLR SVMQSSGSVT QSLRSVMQSS
GSVTQSLRSV MQSSGSVMQS PGGVTQSLRS VTQSPGGVMQ SPGGVMQSPR DVMQSPRDVM
QSPRDVMQSP RGVTQSLGSM MQSPGGVMQS LRSVMQSSGG VTQSLRSVMQ SPGGVMQSPG
GVTQSPRGMI KSPGMMLSPG DVIQSLNSVP QSSRDRMQSA GHVPSASGDA IQSPGGMSPT
SRDRMQSPGG VSLATEDSIQ LPGGVPLSSD VIQSQGGVPL SSKDRMQSPG GVPPSSGDMI
QSQGGVPQSL GDAIQSAGGV PQSSGDAIQS PGGVSLATGD SIQLPGGVPL SSGDVIQSQG
GVPRSSRDRM QSPGGVPPSS GGVIQSPGGV SPASGDAIQS PGSVLPASGD AIQSAGGVLL
ASGDAIQSPR SVPQSPSGTL RSPGNMSESL GDTPNLSGDV SNSPQALLDL ARDRPKSSPN
DVQNRDTPMD ISASSSSSCS ANPQSRQSEF KLDKVPWLTI TDSSARREKS LPQLANPGSA
QIQGQIPQVG VYNRPCLHRL KYFLRPPVHH LFFQTLIPDK DTRESKGQKL EPIPHRRLRM
VTNTIEENFP LGTVQFLMDF VSPQHYPPRE IVAHIVQKIL LSGSETVDVL KEAYMLLMKI
QQLHPANAKT VEWDWKLLTY VMEEEGQTLP GRVLFLRYVV QTLEDDFQQI LRRQRQHLQQ
SIANTVLSCD KQPHNVRDVI KWLVKAVTEN ELTPPQDETQ TSPRTGVLKT SSDHLSPRPN
QNKNTNQLIV CQLQRMLSIA VEVDRTPTCS SNKIAEMMFG FVLDIPERSQ REMFFTTMES
HLLRCKVLEI IFLHSCETPT RLPLSLAQTL YFLNNSTSLL KCQSDKTQWQ TWDELVEHLQ
FLLSSYQHVL REHLRSSVID RKDLIIKRIK PKPQQGDDIT VLDVEKQIEA FRSRLVHILG
EPLVPQLQDK VHLLKLLLFY AADLNPDTEP ASEH
