SIMR1_CAEEL
ID SIMR1_CAEEL Reviewed; 691 AA.
AC O01477;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein simr-1 {ECO:0000305};
DE AltName: Full=siRNA-defective and mortal germline protein 1 {ECO:0000303|PubMed:32338603};
GN Name=simr-1 {ECO:0000303|PubMed:32338603, ECO:0000312|WormBase:C06A5.6};
GN ORFNames=C06A5.6 {ECO:0000312|WormBase:C06A5.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF ALA-11 AND
RP ARG-159.
RX PubMed=32338603; DOI=10.7554/elife.56731;
RA Manage K.I., Rogers A.K., Wallis D.C., Uebel C.J., Anderson D.C.,
RA Nguyen D.A.H., Arca K., Brown K.C., Cordeiro Rodrigues R.J.,
RA de Albuquerque B.F.M., Ketting R.F., Montgomery T.A., Phillips C.M.;
RT "A Tudor domain protein, SIMR-1, promotes siRNA production at piRNA-
RT targeted mRNAs in C. elegans.";
RL Elife 9:0-0(2020).
CC -!- FUNCTION: Acts downstream of piRNA production to promote mediator
CC complex-dependent endogenous siRNA biogenesis from piRNA-target mRNAs
CC in the RNA interference pathway in germ cells (PubMed:32338603). Not
CC required to identify target mRNA by the piRNA pathway
CC (PubMed:32338603). Plays a role in both spermatogenesis and oogenesis
CC and in maintaining fertility over multiple generations, probably by
CC directing mutator-dependent silencing to piRNA-targeted genes
CC (PubMed:32338603). {ECO:0000269|PubMed:32338603}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:32338603}. Note=Localizes to perinuclear foci in
CC germ cells, called SIMR foci, which are distinct from Mutator foci, P
CC granules and Z granules (PubMed:32338603). SIMR foci are stacked, with
CC znfx-1 localizing between simr-1 and pgl-1 (PubMed:32338603). Its
CC localization at SIMR foci is adjacent to the mutator complex protein
CC mut-16 (PubMed:32338603). Perinuclear localization is not dependent on
CC mut-16 (PubMed:32338603). {ECO:0000269|PubMed:32338603}.
CC -!- DOMAIN: Tudor domain specifically binds peptides with symmetrically
CC dimethylated arginines (sDMA) and may facilitate protein-protein
CC interactions (PubMed:32338603). The Tudor domain contains the conserved
CC arginine and aspartic acid residues, which play a structural role, but
CC lacks two of the four conserved aromatic residues, so it is unclear
CC whether it is functional to recognize a methylated substrate
CC (PubMed:32338603). {ECO:0000303|PubMed:32338603}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284601; CCD61216.1; -; Genomic_DNA.
DR PIR; T25519; T25519.
DR RefSeq; NP_491730.1; NM_059329.4.
DR AlphaFoldDB; O01477; -.
DR STRING; 6239.C06A5.6; -.
DR EPD; O01477; -.
DR PaxDb; O01477; -.
DR PeptideAtlas; O01477; -.
DR EnsemblMetazoa; C06A5.6.1; C06A5.6.1; WBGene00015504.
DR EnsemblMetazoa; C06A5.6.2; C06A5.6.2; WBGene00015504.
DR EnsemblMetazoa; C06A5.6.3; C06A5.6.3; WBGene00015504.
DR UCSC; C06A5.6; c. elegans.
DR WormBase; C06A5.6; CE07953; WBGene00015504; simr-1.
DR eggNOG; ENOG502RT5V; Eukaryota.
DR GeneTree; ENSGT00970000196511; -.
DR HOGENOM; CLU_358340_0_0_1; -.
DR InParanoid; O01477; -.
DR OrthoDB; 1788096at2759; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00015504; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:0090727; P:positive regulation of brood size; IMP:UniProtKB.
DR GO; GO:1905881; P:positive regulation of oogenesis; IMP:UniProtKB.
DR GO; GO:0030422; P:siRNA processing; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..691
FT /note="Protein simr-1"
FT /id="PRO_0000450823"
FT DOMAIN 139..204
FT /note="Tudor; degenerate"
FT /evidence="ECO:0000305|PubMed:32338603"
FT REGION 547..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 588..618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 11
FT /note="A->V: Inhibits piRNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:32338603"
FT MUTAGEN 159
FT /note="R->C: Progressive fertility defects at 25 degrees
FT Celsius, with animals becoming sterile after 10 to 11
FT generations. Expressed in the cytoplasm of germ cells, but
FT does not form germline foci. Inhibits piRNA-mediated gene
FT silencing."
FT /evidence="ECO:0000269|PubMed:32338603"
SQ SEQUENCE 691 AA; 76791 MW; 2C8A20C97C69FD49 CRC64;
MSNEKQQWDD AYCLAEEMHD AGRPFKREEL NIADQFICSR FSTIFEYDPY KDTYGFRNQE
LTHAIPVKSK CRHISDVDIL ELKKNTANAD IRFPSYLNIC PVYAMHPFTV LAFDLSKPVP
MGMNASMENA APNMKSPTEA EITPGTIYIF KHRDNKCYRC VILFEDGDNN VSDADRKYMV
AFLDTPQVVS VKLKTLFHLG KFTIESYPCA LYCCRAVGIL EIRKDFGADL NGQINEFYKD
KVKRKSGVHA LIYKKDDRGD KLIFDCPSIL GTSMTMALEI KDVIGHRSVA ENDPTALSYD
ELVSKQLPTV DIDDHNSSVV LDLEESVIAQ ELGSTNGADC PCNNDNIDDF MQSQRQNPLD
NNRDNWDRIN ESRSSMQSFA INQSQAITAN PTPQPTFDES SGEVQTIPES INNLALNGRY
LEDGRGTEEI REERSVESRQ IGNQVVSQAS CNYLEARQNS TQTANAESVC AIISESHAAL
PTDIQVIPSQ HVLNENNHTV LPSVAPIIRN ATGHSHIFGR QIPSPAFRRE SLSSGNSIQV
ATFAATTGPC GSNTSRPTAQ NTANSSINQD MSISNSSTNA RLITIAQDNL NDTENWPNSE
REQSATEMES GAEATTNSAV DEFAQVSDDM KGLADSMINF LRLTANSNNQ DAFKANIFAM
ELISTKIPNQ LTKRFFTLKI AEAKSLAEGF N
