SIM_ARATH
ID SIM_ARATH Reviewed; 127 AA.
AC Q9LZ78; Q680L7;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Cyclin-dependent protein kinase inhibitor SIM {ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
DE AltName: Full=Protein SIAMESE;
GN Name=SIM {ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
GN OrderedLocusNames=At5g04470 {ECO:0000312|Araport:AT5G04470};
GN ORFNames=T32M21.70 {ECO:0000312|EMBL:CAB85553.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 73-127.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10952891; DOI=10.1242/dev.127.18.3931;
RA Walker J.D., Oppenheimer D.G., Concienne J., Larkin J.C.;
RT "SIAMESE, a gene controlling the endoreduplication cell cycle in
RT Arabidopsis thaliana trichomes.";
RL Development 127:3931-3940(2000).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11882294; DOI=10.1016/s0960-9822(02)00693-0;
RA Schnittger A., Schoebinger U., Stierhof Y.-D., Huelskamp M.;
RT "Ectopic B-type cyclin expression induces mitotic cycles in
RT endoreduplicating Arabidopsis trichomes.";
RL Curr. Biol. 12:415-420(2002).
RN [8]
RP FUNCTION, INTERACTION WITH CDKA-1; CYCD2-1; CYCD3-2 AND CYCD4-1,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17098811; DOI=10.1105/tpc.106.044834;
RA Churchman M.L., Brown M.L., Kato N., Kirik V., Huelskamp M., Inze D.,
RA De Veylder L., Walker J.D., Zheng Z., Oppenheimer D.G., Gwin T.,
RA Churchman J., Larkin J.C.;
RT "SIAMESE, a plant-specific cell cycle regulator, controls endoreplication
RT onset in Arabidopsis thaliana.";
RL Plant Cell 18:3145-3157(2006).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17764505; DOI=10.1111/j.1365-313x.2007.03243.x;
RA Marks M.D., Gilding E., Wenger J.P.;
RT "Genetic interaction between glabra3-shapeshifter and siamese in
RT Arabidopsis thaliana converts trichome precursors into cells with
RT meristematic activity.";
RL Plant J. 52:352-361(2007).
RN [10]
RP INDUCTION.
RX PubMed=19576768; DOI=10.1016/j.cub.2009.05.059;
RA Achard P., Gusti A., Cheminant S., Alioua M., Dhondt S., Coppens F.,
RA Beemster G.T., Genschik P.;
RT "Gibberellin signaling controls cell proliferation rate in Arabidopsis.";
RL Curr. Biol. 19:1188-1193(2009).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19717615; DOI=10.1105/tpc.109.068387;
RA Breuer C., Kawamura A., Ichikawa T., Tominaga-Wada R., Wada T., Kondou Y.,
RA Muto S., Matsui M., Sugimoto K.;
RT "The trihelix transcription factor GTL1 regulates ploidy-dependent cell
RT growth in the Arabidopsis trichome.";
RL Plant Cell 21:2307-2322(2009).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20194967; DOI=10.1534/genetics.109.113274;
RA Kasili R., Walker J.D., Simmons L.A., Zhou J., De Veylder L., Larkin J.C.;
RT "SIAMESE cooperates with the CDH1-like protein CCS52A1 to establish
RT endoreplication in Arabidopsis thaliana trichomes.";
RL Genetics 185:257-268(2010).
RN [13]
RP INTERACTION WITH CDKB1-1.
RX PubMed=20706207; DOI=10.1038/msb.2010.53;
RA Van Leene J., Hollunder J., Eeckhout D., Persiau G., Van De Slijke E.,
RA Stals H., Van Isterdael G., Verkest A., Neirynck S., Buffel Y., De Bodt S.,
RA Maere S., Laukens K., Pharazyn A., Ferreira P.C.G., Eloy N., Renne C.,
RA Meyer C., Faure J.-D., Steinbrenner J., Beynon J., Larkin J.C.,
RA Van de Peer Y., Hilson P., Kuiper M., De Veylder L., Van Onckelen H.,
RA Inze D., Witters E., De Jaeger G.;
RT "Targeted interactomics reveals a complex core cell cycle machinery in
RT Arabidopsis thaliana.";
RL Mol. Syst. Biol. 6:397-397(2010).
RN [14]
RP DISRUPTION PHENOTYPE.
RX PubMed=21558384; DOI=10.1242/dev.058982;
RA Kasili R., Huang C.C., Walker J.D., Simmons L.A., Zhou J., Faulk C.,
RA Huelskamp M., Larkin J.C.;
RT "BRANCHLESS TRICHOMES links cell shape and cell cycle control in
RT Arabidopsis trichomes.";
RL Development 138:2379-2388(2011).
RN [15]
RP INDUCTION BY ZEOCIN.
RX PubMed=21613568; DOI=10.1073/pnas.1103584108;
RA Adachi S., Minamisawa K., Okushima Y., Inagaki S., Yoshiyama K., Kondou Y.,
RA Kaminuma E., Kawashima M., Toyoda T., Matsui M., Kurihara D., Matsunaga S.,
RA Umeda M.;
RT "Programmed induction of endoreduplication by DNA double-strand breaks in
RT Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:10004-10009(2011).
RN [16]
RP FUNCTION, AND INTERACTION WITH CPR5.
RX PubMed=25455564; DOI=10.1016/j.chom.2014.10.005;
RA Wang S., Gu Y., Zebell S.G., Anderson L.K., Wang W., Mohan R., Dong X.;
RT "A noncanonical role for the CKI-RB-E2F cell-cycle signaling pathway in
RT plant effector-triggered immunity.";
RL Cell Host Microbe 16:787-794(2014).
RN [17]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24399300; DOI=10.1105/tpc.113.118943;
RA Yi D., Alvim Kamei C.L., Cools T., Vanderauwera S., Takahashi N.,
RA Okushima Y., Eekhout T., Yoshiyama K.O., Larkin J., Van den Daele H.,
RA Conklin P., Britt A., Umeda M., De Veylder L.;
RT "The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5
RT and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen
RT species.";
RL Plant Cell 26:296-309(2014).
RN [18]
RP FUNCTION, GENE FAMILY, NOMENCLATURE, AND INTERACTION WITH CDKA-1 AND
RP CDKB1-1.
RX PubMed=26546445; DOI=10.1105/tpc.15.00489;
RA Kumar N., Harashima H., Kalve S., Bramsiepe J., Wang K., Sizani B.L.,
RA Bertrand L.L., Johnson M.C., Faulk C., Dale R., Simmons L.A.,
RA Churchman M.L., Sugimoto K., Kato N., Dasanayake M., Beemster G.,
RA Schnittger A., Larkin J.C.;
RT "Functional conservation in the SIAMESE-RELATED family of cyclin-dependent
RT kinase inhibitors in land plants.";
RL Plant Cell 27:3065-3080(2015).
CC -!- FUNCTION: Cyclin-dependent protein kinase (CDK) inhibitor that
CC functions as a repressor of mitosis in the endoreduplication cell cycle
CC (PubMed:10952891, PubMed:11882294, PubMed:17098811, PubMed:17764505,
CC PubMed:19717615, PubMed:20194967). Inhibits the kinase activity of
CC CYCD3-1/CDKA-1, CYCD2-1/CDKA-1 and CYCB1-1/CDKB1-1 complexes in a dose
CC dependent manner (PubMed:26546445). Cooperates with SMR1 and SMR2 to
CC promote endoreplication during leaf development (PubMed:26546445).
CC Required for normal trichome endoreplicating cell cycles
CC (PubMed:10952891, PubMed:11882294, PubMed:17098811, PubMed:17764505,
CC PubMed:19717615, PubMed:20194967). Positive regulator of effector-
CC triggered immunity (ETI) (PubMed:25455564).
CC {ECO:0000269|PubMed:10952891, ECO:0000269|PubMed:11882294,
CC ECO:0000269|PubMed:17098811, ECO:0000269|PubMed:17764505,
CC ECO:0000269|PubMed:19717615, ECO:0000269|PubMed:20194967,
CC ECO:0000269|PubMed:25455564, ECO:0000269|PubMed:26546445}.
CC -!- SUBUNIT: Interacts with CDKA-1 (PubMed:17098811, PubMed:26546445).
CC Interacts with CYCD2-1, CYCD3-2 and CYCD4-1 (PubMed:17098811).
CC Interacts with CDKB1-1 (PubMed:20706207, PubMed:26546445). Interacts
CC with CPR5 (PubMed:25455564). {ECO:0000269|PubMed:17098811,
CC ECO:0000269|PubMed:20706207, ECO:0000269|PubMed:25455564,
CC ECO:0000269|PubMed:26546445}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17098811}.
CC -!- TISSUE SPECIFICITY: Expressed in the shoot apical meristem, leaf
CC primordia and the elongation zone of the root.
CC {ECO:0000269|PubMed:17098811, ECO:0000269|PubMed:24399300}.
CC -!- INDUCTION: Down-regulated by gibberellin (PubMed:19576768). Up-
CC regulated by zeocin treatment (PubMed:21613568).
CC {ECO:0000269|PubMed:19576768, ECO:0000269|PubMed:21613568}.
CC -!- DISRUPTION PHENOTYPE: Multicellular trichomes with nuclei showing
CC reduced levels of endoreduplication. {ECO:0000269|PubMed:10952891,
CC ECO:0000269|PubMed:11882294, ECO:0000269|PubMed:17098811,
CC ECO:0000269|PubMed:17764505, ECO:0000269|PubMed:19717615,
CC ECO:0000269|PubMed:20194967, ECO:0000269|PubMed:21558384}.
CC -!- MISCELLANEOUS: Plants over-expressing SIM are dwarf with serrated
CC leaves containing enlarged cells with increased levels of nuclear DNA.
CC {ECO:0000305|PubMed:17098811}.
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DR EMBL; AL162875; CAB85553.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90749.1; -; Genomic_DNA.
DR EMBL; BT009698; AAP88332.1; -; mRNA.
DR EMBL; AY085204; AAM61754.1; -; mRNA.
DR EMBL; AK175850; BAD43613.1; -; mRNA.
DR PIR; T48443; T48443.
DR RefSeq; NP_196067.1; NM_120529.3.
DR AlphaFoldDB; Q9LZ78; -.
DR BioGRID; 15605; 7.
DR IntAct; Q9LZ78; 10.
DR STRING; 3702.AT5G04470.1; -.
DR PaxDb; Q9LZ78; -.
DR PRIDE; Q9LZ78; -.
DR ProteomicsDB; 234580; -.
DR EnsemblPlants; AT5G04470.1; AT5G04470.1; AT5G04470.
DR GeneID; 830326; -.
DR Gramene; AT5G04470.1; AT5G04470.1; AT5G04470.
DR KEGG; ath:AT5G04470; -.
DR Araport; AT5G04470; -.
DR TAIR; locus:2184422; AT5G04470.
DR eggNOG; ENOG502R1UX; Eukaryota.
DR HOGENOM; CLU_130615_0_0_1; -.
DR InParanoid; Q9LZ78; -.
DR OMA; HKIPPTT; -.
DR OrthoDB; 1569151at2759; -.
DR PhylomeDB; Q9LZ78; -.
DR PRO; PR:Q9LZ78; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZ78; baseline and differential.
DR Genevisible; Q9LZ78; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; NAS:TAIR.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:TAIR.
DR GO; GO:0045786; P:negative regulation of cell cycle; IMP:TAIR.
DR GO; GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; NAS:TAIR.
DR GO; GO:0045839; P:negative regulation of mitotic nuclear division; IMP:TAIR.
DR GO; GO:0032875; P:regulation of DNA endoreduplication; IEA:InterPro.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR InterPro; IPR040389; SMR.
DR PANTHER; PTHR33142; PTHR33142; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Nucleus; Protein kinase inhibitor; Reference proteome.
FT CHAIN 1..127
FT /note="Cyclin-dependent protein kinase inhibitor SIM"
FT /id="PRO_0000418063"
FT REGION 21..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..62
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 127 AA; 14091 MW; 3CD095F760CBE781 CRC64;
MDLDLIQDLP ILNFPPAIKI RANTNRDDDG GGCTTPTSSD HKIPPTTATT PPPPPQKPRP
PSTPSSLGIR SCKRKLMTSL SKYEIIVNKD EIERFFSSVY NQTMASSTTT AITVAKRRRS
FRSCSRR
