SINE2_ARATH
ID SINE2_ARATH Reviewed; 554 AA.
AC Q9SQR5;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Protein SINE2 {ECO:0000303|PubMed:24891605};
GN Name=SINE2 {ECO:0000303|PubMed:24891605};
GN OrderedLocusNames=At3g03970 {ECO:0000312|Araport:AT3G03970};
GN ORFNames=T11I18.8 {ECO:0000312|EMBL:AAF05856.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, INTERACTION WITH SINE1 AND
RP SINE2, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=24891605; DOI=10.1083/jcb.201401138;
RA Zhou X., Graumann K., Wirthmueller L., Jones J.D., Meier I.;
RT "Identification of unique SUN-interacting nuclear envelope proteins with
RT diverse functions in plants.";
RL J. Cell Biol. 205:677-692(2014).
RN [6]
RP REVIEW.
RX PubMed=25740919; DOI=10.1093/jxb/erv082;
RA Zhou X., Graumann K., Meier I.;
RT "The plant nuclear envelope as a multifunctional platform LINCed by SUN and
RT KASH.";
RL J. Exp. Bot. 66:1649-1659(2015).
CC -!- FUNCTION: Plays a role in innate immunity against the oomycete pathogen
CC A.arabidopsidis (Hpa). {ECO:0000269|PubMed:24891605}.
CC -!- SUBUNIT: Interacts with SUN1 and SUN2. {ECO:0000269|PubMed:24891605}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:24891605};
CC Single-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in epidermal cells, mesophyll cells,
CC trichomes and root cells. {ECO:0000269|PubMed:24891605}.
CC -!- DOMAIN: The KASH domain, which contains a transmembrane domain,
CC mediates the nuclear envelope targeting and is involved in the binding
CC to the SUN proteins. {ECO:0000305|PubMed:24891605}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. More susceptible to the
CC oomycete pathogen H.arabidopsidis (Hpa) infection.
CC {ECO:0000269|PubMed:24891605}.
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DR EMBL; AC011698; AAF05856.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74019.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74020.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74021.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64427.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64428.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64429.1; -; Genomic_DNA.
DR EMBL; AK117787; BAC42434.1; -; mRNA.
DR EMBL; BT005915; AAO64850.1; -; mRNA.
DR RefSeq; NP_001319464.1; NM_001337495.1.
DR RefSeq; NP_001326457.1; NM_001337497.1.
DR RefSeq; NP_001326458.1; NM_001337496.1.
DR RefSeq; NP_187047.1; NM_111268.3.
DR RefSeq; NP_974214.1; NM_202485.2.
DR RefSeq; NP_974215.1; NM_202486.2.
DR AlphaFoldDB; Q9SQR5; -.
DR STRING; 3702.AT3G03970.3; -.
DR iPTMnet; Q9SQR5; -.
DR PaxDb; Q9SQR5; -.
DR PRIDE; Q9SQR5; -.
DR ProteomicsDB; 232508; -.
DR EnsemblPlants; AT3G03970.1; AT3G03970.1; AT3G03970.
DR EnsemblPlants; AT3G03970.2; AT3G03970.2; AT3G03970.
DR EnsemblPlants; AT3G03970.3; AT3G03970.3; AT3G03970.
DR EnsemblPlants; AT3G03970.4; AT3G03970.4; AT3G03970.
DR EnsemblPlants; AT3G03970.5; AT3G03970.5; AT3G03970.
DR EnsemblPlants; AT3G03970.6; AT3G03970.6; AT3G03970.
DR GeneID; 819552; -.
DR Gramene; AT3G03970.1; AT3G03970.1; AT3G03970.
DR Gramene; AT3G03970.2; AT3G03970.2; AT3G03970.
DR Gramene; AT3G03970.3; AT3G03970.3; AT3G03970.
DR Gramene; AT3G03970.4; AT3G03970.4; AT3G03970.
DR Gramene; AT3G03970.5; AT3G03970.5; AT3G03970.
DR Gramene; AT3G03970.6; AT3G03970.6; AT3G03970.
DR KEGG; ath:AT3G03970; -.
DR Araport; AT3G03970; -.
DR TAIR; locus:2095963; AT3G03970.
DR eggNOG; ENOG502QQPA; Eukaryota.
DR HOGENOM; CLU_028938_0_0_1; -.
DR InParanoid; Q9SQR5; -.
DR OMA; SEAKSHM; -.
DR OrthoDB; 385794at2759; -.
DR PhylomeDB; Q9SQR5; -.
DR PRO; PR:Q9SQR5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SQR5; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0032797; C:SMN complex; IBA:GO_Central.
DR GO; GO:0061760; P:antifungal innate immune response; IMP:UniProtKB.
DR GO; GO:0000387; P:spliceosomal snRNP assembly; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Immunity; Innate immunity; Membrane; Nucleus; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..554
FT /note="Protein SINE2"
FT /id="PRO_0000441682"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 509..554
FT /note="KASH"
FT /evidence="ECO:0000305|PubMed:24891605"
FT REGION 17..290
FT /note="ARMADILLO-type fold"
FT /evidence="ECO:0000255"
FT REGION 306..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 411..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 465..487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 551..554
FT /note="Required for nuclear localization"
FT /evidence="ECO:0000269|PubMed:24891605"
FT COMPBIAS 308..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..442
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 554 AA; 61442 MW; F2DEBB2EC182FAFB CRC64;
MGRNLGSAFR QELANLDKDP DSHKTAMSNL RSIVKDLDAK VVHVFVAQLS DVKEIGLESG
GYTVSLFEDL ARAHGVKIAP HIDIIMPAII RTLSSSEGSL RVQQACSRAV AAMARYGIDP
TTPEDKKTNV IHSLCKPLSD SLIDSQHQQH LALGSALCLK SLVDCDNWRS ASSEMVNNVC
QSLAVALEAT SSEAKSHMAL VMALSKHNPF TVEAYARLFV KSGLRILDLG VVEGDSQKRL
LAIQMLNFLM KNLNPKSISS ELELIYQEME KYQKDQHYVK MAAHETMRQA ERLICEADPM
FDAENCKPRN SLSGSVKSTS SLREHDGSVY SRQDRSYVND QDEYDVLFSG VASGRTLVSG
SPLVTFGDNN QETGFVIESP RIGDQIQCSG VENGNIESVW FHQRNRSSEF NESVCSRTNR
SRSSRRNTKK RQSGDICSKH HRHGFAQDPF TELLDNRQQL LQYSETSSSS SIYDTSGTTT
PTNTTEDICE KPKTDLDSEA KLKTVETELD PRLGRSKGVL KLGLSVFSIA VAGFASFMWM
YLQDDMMPPH LVPT
