SINGL_DROME
ID SINGL_DROME Reviewed; 176 AA.
AC Q9VXD1; G2J5W8;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein singles bar {ECO:0000303|PubMed:17537424};
GN Name=sing {ECO:0000303|PubMed:17537424, ECO:0000312|FlyBase:FBgn0261245};
GN ORFNames=CG13011 {ECO:0000312|FlyBase:FBgn0261245};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AEM92656.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-176.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AEM92656.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP ALA-46; 142-TRP--ARG-176 AND 160-LEU--ARG-176.
RX PubMed=17537424; DOI=10.1016/j.ydbio.2007.04.045;
RA Estrada B., Maeland A.D., Gisselbrecht S.S., Bloor J.W., Brown N.H.,
RA Michelson A.M.;
RT "The MARVEL domain protein, Singles Bar, is required for progression past
RT the pre-fusion complex stage of myoblast fusion.";
RL Dev. Biol. 307:328-339(2007).
RN [5] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY MEF2, AND DISRUPTION PHENOTYPE.
RX PubMed=25797154; DOI=10.1016/j.ydbio.2015.02.026;
RA Brunetti T.M., Fremin B.J., Cripps R.M.;
RT "Identification of singles bar as a direct transcriptional target of
RT Drosophila Myocyte enhancer factor-2 and a regulator of adult myoblast
RT fusion.";
RL Dev. Biol. 401:299-309(2015).
CC -!- FUNCTION: Essential for myoblast fusion in developing embryos and
CC pupae, and consequently is essential for muscle formation in adults
CC (PubMed:17537424, PubMed:25797154). Required for progression past the
CC pre-fusion complex stage of myoblast fusion (PubMed:17537424).
CC {ECO:0000269|PubMed:17537424, ECO:0000269|PubMed:25797154}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: In embryos, expressed in the muscle founder cells
CC (FCs) and the fusion-competent myoblasts (FCMs) (PubMed:17537424).
CC Expressed in myoblasts of stage 13 embryos (PubMed:17537424,
CC PubMed:25797154). Expressed in the visceral mesoderm from stage 10 and
CC the somatic mesoderm from stage 11, and expression persists until stage
CC 13 (PubMed:17537424). {ECO:0000269|PubMed:17537424,
CC ECO:0000269|PubMed:25797154}.
CC -!- INDUCTION: By Mef2 during myoblast fusion in developing muscles of
CC embryos and pupae. {ECO:0000269|PubMed:25797154}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in pupae is adult lethal
CC (PubMed:25797154). Pupae develop to the pharate adult stage and die
CC before eclosion from the pupal case (PubMed:25797154). In pupae,
CC fusion-competent myoblasts (FCMs) fail to fuse with the muscle founder
CC templates (PubMed:25797154). Pharate adults display adherent muscle
CC formation; only partially developed dorsal longitudinal muscles appear
CC to be present but they are smaller and their nuclei are reduced in
CC number and clustered together (PubMed:25797154). RNAi-mediated
CC knockdown in embryos reduces myoblast fusion (PubMed:17537424). In RNAi
CC pupae, there is no effect on myoblast proliferation, migration of the
CC FCMs to the founder templates and initiation of myoblast fusion
CC (PubMed:25797154). {ECO:0000269|PubMed:17537424,
CC ECO:0000269|PubMed:25797154}.
CC -!- MISCELLANEOUS: Named 'singles bar' based upon the accumulation of
CC single myoblasts in mutant embryos. {ECO:0000303|PubMed:17537424}.
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DR EMBL; AE014298; AAF48643.1; -; Genomic_DNA.
DR EMBL; BT128825; AEM92656.1; -; mRNA.
DR RefSeq; NP_573146.1; NM_132918.2.
DR AlphaFoldDB; Q9VXD1; -.
DR STRING; 7227.FBpp0074092; -.
DR PaxDb; Q9VXD1; -.
DR EnsemblMetazoa; FBtr0074318; FBpp0074092; FBgn0261245.
DR GeneID; 32644; -.
DR KEGG; dme:Dmel_CG13011; -.
DR UCSC; CG13011-RA; d. melanogaster.
DR CTD; 32644; -.
DR FlyBase; FBgn0261245; sing.
DR VEuPathDB; VectorBase:FBgn0261245; -.
DR eggNOG; ENOG502S24W; Eukaryota.
DR HOGENOM; CLU_119831_0_0_1; -.
DR InParanoid; Q9VXD1; -.
DR OMA; RCCTCIH; -.
DR OrthoDB; 1376934at2759; -.
DR PhylomeDB; Q9VXD1; -.
DR BioGRID-ORCS; 32644; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32644; -.
DR PRO; PR:Q9VXD1; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0261245; Expressed in embryonic myoblast (Drosophila) and 18 other tissues.
DR Genevisible; Q9VXD1; DM.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0007527; P:adult somatic muscle development; IMP:FlyBase.
DR GO; GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
DR GO; GO:0007520; P:myoblast fusion; IMP:FlyBase.
DR InterPro; IPR008253; Marvel.
DR Pfam; PF01284; MARVEL; 1.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Membrane; Myogenesis; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..176
FT /note="Protein singles bar"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438867"
FT TRANSMEM 13..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 30..173
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT MUTAGEN 46
FT /note="A->V: In sing-22; myoblast fusion is abolished and
FT consequently multinucleated myofibers fail to form during
FT embryonic muscle development."
FT /evidence="ECO:0000269|PubMed:17537424"
FT MUTAGEN 142..176
FT /note="Missing: In sing-23; myoblast fusion is abolished
FT and consequently multinucleated myofibers fail to form
FT during embryonic muscle development."
FT /evidence="ECO:0000269|PubMed:17537424"
FT MUTAGEN 160..176
FT /note="Missing: In sing-27; myoblast fusion is abolished
FT and consequently multinucleated myofibers fail to form
FT during embryonic muscle development."
FT /evidence="ECO:0000269|PubMed:17537424"
SQ SEQUENCE 176 AA; 19460 MW; FDCA1AADE316A1D1 CRC64;
MASFGVRGMG QPLGIRICCC RVCTCINFGF VLSRIGLLKL MQLGLAMLCE GLLIRYGVPY
ADSIGQALTS FLATTGHCFT TTGILLLCYA FSDKSYSLIR QSLFETLFNG LASCMYFSSS
SYMGFACVVW LHPQFLVRPG FWAYPAMTAC YYMGYAAGIL HALDAYLAFR HFRGAR
