ID   SIP1_SCHPO              Reviewed;        1919 AA.
AC   O42998; O13661;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Pof6 interactor protein 1;
GN   Name=sip1; ORFNames=pi070, SPBC27B12.08;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10620777;
RX   DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA   Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA   Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA   Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT   "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT   yeast chromosome II: sequence analysis and characterization of the genomic
RT   DNA and cDNAs encoded on the segment.";
RL   Yeast 16:71-80(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-48, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH POF6 AND SKP1, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19243310; DOI=10.1042/bj20081659;
RA   Jourdain I., Spielewoy N., Thompson J., Dhut S., Yates J.R., Toda T.;
RT   "Identification of a conserved F-box protein 6 interactor essential for
RT   endocytosis and cytokinesis in fission yeast.";
RL   Biochem. J. 420:169-177(2009).
CC   -!- FUNCTION: Required for endocytosis. Also involved in cytokinesis and
CC       cell division. {ECO:0000269|PubMed:19243310}.
CC   -!- SUBUNIT: Forms a complex with pof6 and skp1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11859360,
CC       ECO:0000269|PubMed:16823372}. Cytoplasmic vesicle
CC       {ECO:0000269|PubMed:11859360}. Nucleus {ECO:0000269|PubMed:11859360,
CC       ECO:0000269|PubMed:16823372}. Golgi apparatus
CC       {ECO:0000269|PubMed:16823372}. Note=Localizes to endocytotic vesicles.
CC       {ECO:0000269|PubMed:11859360}.
CC   -!- DISRUPTION PHENOTYPE: Defective in endocytosis. Displays substantially
CC       delayed constriction of the actomyosin ring and dissociation of type 2
CC       myosin and septum materials at the end of cytokinesis.
CC       {ECO:0000269|PubMed:19243310}.
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DR   EMBL; AB004539; BAA21452.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAA16903.1; -; Genomic_DNA.
DR   PIR; T40032; T40032.
DR   RefSeq; NP_595540.1; NM_001021451.2.
DR   AlphaFoldDB; O42998; -.
DR   BioGRID; 276846; 14.
DR   STRING; 4896.SPBC27B12.08.1; -.
DR   iPTMnet; O42998; -.
DR   MaxQB; O42998; -.
DR   PaxDb; O42998; -.
DR   PRIDE; O42998; -.
DR   EnsemblFungi; SPBC27B12.08.1; SPBC27B12.08.1:pep; SPBC27B12.08.
DR   PomBase; SPBC27B12.08; sip1.
DR   VEuPathDB; FungiDB:SPBC27B12.08; -.
DR   eggNOG; KOG1822; Eukaryota.
DR   HOGENOM; CLU_235407_0_0_1; -.
DR   InParanoid; O42998; -.
DR   OMA; GIVCAHV; -.
DR   PhylomeDB; O42998; -.
DR   PRO; PR:O42998; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0030139; C:endocytic vesicle; IDA:PomBase.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0006897; P:endocytosis; IMP:PomBase.
DR   GO; GO:0006886; P:intracellular protein transport; IC:PomBase.
DR   GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISO:PomBase.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR040108; Laa1/Sip1/HEATR5.
DR   PANTHER; PTHR21663; PTHR21663; 1.
DR   SUPFAM; SSF48371; SSF48371; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Nucleus;
KW   Phosphoprotein; Reference proteome; Repeat.
FT   CHAIN           1..1919
FT                   /note="Pof6 interactor protein 1"
FT                   /id="PRO_0000372352"
FT   REPEAT          106..145
FT                   /note="HEAT 1"
FT   REPEAT          166..204
FT                   /note="HEAT 2"
FT   REPEAT          378..416
FT                   /note="HEAT 3"
FT   REPEAT          457..497
FT                   /note="HEAT 4"
FT   REPEAT          783..820
FT                   /note="HEAT 5"
FT   REPEAT          854..892
FT                   /note="HEAT 6"
FT   REPEAT          894..930
FT                   /note="HEAT 7"
FT   REPEAT          935..972
FT                   /note="HEAT 8"
FT   REPEAT          1265..1301
FT                   /note="HEAT 9"
FT   REPEAT          1336..1378
FT                   /note="HEAT 10"
FT   REPEAT          1526..1552
FT                   /note="HEAT 11"
FT   REPEAT          1553..1589
FT                   /note="HEAT 12"
FT   REPEAT          1674..1714
FT                   /note="HEAT 13"
FT   REPEAT          1722..1760
FT                   /note="HEAT 14"
FT   REPEAT          1767..1806
FT                   /note="HEAT 15"
FT   REPEAT          1832..1869
FT                   /note="HEAT 16"
FT   REGION          256..278
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         45
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         48
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   CONFLICT        14..125
FT                   /note="Missing (in Ref. 1; BAA21452)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1919 AA;  217691 MW;  77EE67F005F318F0 CRC64;
     MSLASLPLEK LTKVADVDKR ETIYIDLLNY EKQLRNLNKN TREDSLQTNL NPLLSLIKKY
     GNFIAYPTRK LLSNCIIALL KGLEGYHQHR VLLYFVDSIH EKKDYIEIIN VLSVLTSCLE
     EYGNQINQQE TFIRFFFKYS KTNLSYGVRS IALDGLSKCI ETSIQSTNED LIKEIWKCIK
     SNLNTSSTNV LLSALRCAYY ILGSSRLRNS ELEWFKSFLF KKSSIDNSLL HSSLGRCFSA
     LASAQADKSI EAAANAVPST PADDENNEEV ENRSTTRRFD ETESFRHALR ILVENYHSKH
     AKYESSKAVL VFAVKHLFLM QRPSFVSLNY TTIVDIILLH QSHSTSKEWL SSLEQYHCTY
     LFRDVIGRRF LDQSSELQGI EILLKRVLEP YLKDQSKISE IAVKTAMAST ADILSRSKGS
     ATALYSQLLD TTLQILYLGK DSLKNEVAWL LRCLLSVMPS PLMPTLLALF NRLELEFKHL
     RSKPKEVSPK LNVGIFNNLT LSLMSIYVAA EENPLYVASS IFSKLFNFSL DLIKSSSKLD
     LLAAQAQIKT GWRLLSAYVS FGPSFTRMQL SQLLILWKNA LSRVPSKIHS QNFMETNADM
     FNRFEALQCL LSFLEYNKIL FTPDVRKRVI IFLNNCLKFY KAFSLTKRLE ITMPIPASDY
     SHLELRSLLL ARIFQCFFEL SQLSGMSLDD QELLSTTVST IAELSSLQGY DTADEIVQAK
     AFTSPRVIPG IGSRFYAQPE EEFQTTMDDI LPCYICESSS DSLSVISRSN SAKLPYVPPS
     LTSLIDNAIQ LFASMFCHQP PKVMESTLEI MVASVTNLKS GRDILRYKAV TANTLFSIYG
     FLSAFDNKKV TLPDKVVSLM IELLENLTAV NDPKLIPYIS KSYAFLAYLY QSKPTSVLDT
     IIKRAAESGD PNCRAVLILA VGHILKKVGS GVPQTTLITA YQLLKVFNTD SNDLVRECSI
     RASSVSLGSI AKTIPSVLTA QTRILLSYML LPECDIDVQL LKKRNSLFFP STLFANYLDC
     LINELGPNLR SDSSTSHFAF DLAHQLLLLS RDTGDNIIHV YKCYQHLYLF TPLETNSDFF
     IRELCGNIID LKQPKRREIS IEVLYQILLQ NHEFSESCYK RHFDRLIWQS LDTTPDNKLL
     KNILYIWLND YKDSDIHSWV NILLFLVGIK NTSSSERSNE QDIQNNLDED TVSLAYGTSE
     NASNPLFQKN YRWQTQFYAA TCLKTCISEV MAENKEPAKY LISRISDLVR AAFVLSTSDN
     FYLKQGGFEL LDTIITDFSN VMDPDFDDVS LLDQFQAQIS SAFSSAFVDD SSPEIVAMSL
     NIASNFIGTG LLKTESQAAK ILNLLKEALE SSLPERAGFE ENRHFKYDSK YFLRIATLSA
     WASLLVHSAN VSYLKEFLSS NIRRLSSHWI MALRGYSRLQ FGPSLVKDFV ADTSFTRVQS
     INPKILLNIY EKSWLNIAES LTILLSTDAN LIYGILSGDE LSLSEEDVFF QDNINYASNR
     YSFNFFLFSV CFQSLLIPST LQGFRNPVFR IMRIMTEVLT EELCTKVLYD HNVFPEVVDL
     LVRLILTEDW ETKASIVIFV KKLALANPAH NDLKFCETDS VTSMEPTVSE SVEMLFQLVK
     ILTLALTVRV PGLRSDPAES ATNKKASEFV ILCFNAFLDV SNIFPAIVRV DLFATAMHVY
     EVIMDDQQLL KNSKQELLAA LRKLLSTMVE QNDHTCTTLI YTLFEHLLNI YKETINDSDK
     KEKNETAFMS MVLVLTLAAP ILDSEQLVVH DFLEELFSSL KSSEEDFALR TRCVTSLMLV
     NSKHPSMSAL CRFIIYKSVT MLQNGEVLKS GDYVTEFIPA LLDMHVHIPE EKKKSFLSMS
     IPIAAHFSIL VEHADARKRI GEKLLKIMAI QPDEAKAIIQ QLSPRQKKCV EEILIQNVE