SIP21_ARATH
ID SIP21_ARATH Reviewed; 237 AA.
AC Q9M1K3;
DT 27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Probable aquaporin SIP2-1;
DE AltName: Full=Small basic intrinsic protein 2-1;
DE Short=AtSIP2;1;
GN Name=SIP2-1; OrderedLocusNames=At3g56950; ORFNames=F24I3.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=11806824; DOI=10.1186/gb-2001-3-1-research0001;
RA Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.;
RT "From genome to function: the Arabidopsis aquaporins.";
RL Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16223486; DOI=10.1016/j.febslet.2005.09.076;
RA Ishikawa F., Suga S., Uemura T., Sato M.H., Maeshima M.;
RT "Novel type aquaporin SIPs are mainly localized to the ER membrane and show
RT cell-specific expression in Arabidopsis thaliana.";
RL FEBS Lett. 579:5814-5820(2005).
CC -!- FUNCTION: Water channel required to facilitate the transport of water
CC across cell membrane. Inactive in yeast cells.
CC {ECO:0000269|PubMed:16223486}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M1K3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in dividing cells and elongating regions
CC of the root tips, emerging lateral roots, root steles, cotyledons, main
CC veins of the rosette leaves, vascular tissues of the flower petals,
CC stigma, stamens (anthers and filaments), pollen and the top and bottom
CC (receptacle) of siliques. {ECO:0000269|PubMed:11806824,
CC ECO:0000269|PubMed:16223486}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala/Leu (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. SIP (TC
CC 1.A.8.10) subfamily. {ECO:0000305}.
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DR EMBL; AL138655; CAB72165.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79591.1; -; Genomic_DNA.
DR EMBL; AF387020; AAK62465.1; -; mRNA.
DR EMBL; BT006280; AAP13388.1; -; mRNA.
DR EMBL; AY087153; AAM64711.1; -; mRNA.
DR PIR; T47755; T47755.
DR RefSeq; NP_191254.1; NM_115554.4. [Q9M1K3-1]
DR AlphaFoldDB; Q9M1K3; -.
DR SMR; Q9M1K3; -.
DR BioGRID; 10178; 11.
DR IntAct; Q9M1K3; 11.
DR STRING; 3702.AT3G56950.2; -.
DR PaxDb; Q9M1K3; -.
DR PRIDE; Q9M1K3; -.
DR EnsemblPlants; AT3G56950.1; AT3G56950.1; AT3G56950. [Q9M1K3-1]
DR GeneID; 824862; -.
DR Gramene; AT3G56950.1; AT3G56950.1; AT3G56950. [Q9M1K3-1]
DR KEGG; ath:AT3G56950; -.
DR Araport; AT3G56950; -.
DR eggNOG; KOG0223; Eukaryota.
DR HOGENOM; CLU_100006_0_0_1; -.
DR InParanoid; Q9M1K3; -.
DR OMA; HGGPAVY; -.
DR PhylomeDB; Q9M1K3; -.
DR PRO; PR:Q9M1K3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1K3; baseline and differential.
DR Genevisible; Q9M1K3; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR044226; SIP2-1-like.
DR PANTHER; PTHR47720; PTHR47720; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..237
FT /note="Probable aquaporin SIP2-1"
FT /id="PRO_0000064075"
FT TRANSMEM 15..35
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 69..71
FT /note="NPA 1"
FT MOTIF 180..182
FT /note="NPA 2"
SQ SEQUENCE 237 AA; 25945 MW; D639CBA427151042 CRC64;
MGRIGLVVTD LVLSFMWIWA GVLVNILVHG VLGFSRTDPS GEIVRYLFSI ISMFIFAYLQ
QATKGGLYNP LTALAAGVSG GFSSFIFSVF VRIPVEVIGS ILAVKHIIHV FPEIGKGPKL
NVAIHHGALT EGILTFFIVL LSMGLTRKIP GSFFMKTWIG SLAKLTLHIL GSDLTGGCMN
PAAVMGWAYA RGEHITKEHL LVYWLGPVKA TLLAVWFFKV VFKPLTEEQE KPKAKSE
