BH130_ARATH
ID BH130_ARATH Reviewed; 359 AA.
AC Q66GR3; O48535; Q3E6N5;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transcription factor bHLH130;
DE AltName: Full=Basic helix-loop-helix protein 130;
DE Short=AtbHLH130;
DE Short=bHLH 130;
DE AltName: Full=Transcription factor EN 69;
DE AltName: Full=bHLH transcription factor bHLH130;
GN Name=BHLH130; Synonyms=EN69; OrderedLocusNames=At2g42280;
GN ORFNames=T24P15.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q66GR3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q66GR3-2; Sequence=VSP_036104, VSP_036105;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB88652.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002561; AAB88652.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10099.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10100.1; -; Genomic_DNA.
DR EMBL; BT015339; AAU05462.1; -; mRNA.
DR EMBL; BT015712; AAU45210.1; -; mRNA.
DR EMBL; AK228981; BAF00869.1; -; mRNA.
DR PIR; T00937; T00937.
DR RefSeq; NP_181757.2; NM_129790.5. [Q66GR3-1]
DR RefSeq; NP_973670.1; NM_201941.2. [Q66GR3-2]
DR AlphaFoldDB; Q66GR3; -.
DR SMR; Q66GR3; -.
DR BioGRID; 4166; 33.
DR IntAct; Q66GR3; 29.
DR STRING; 3702.AT2G42280.1; -.
DR iPTMnet; Q66GR3; -.
DR PaxDb; Q66GR3; -.
DR PRIDE; Q66GR3; -.
DR ProteomicsDB; 240793; -. [Q66GR3-1]
DR EnsemblPlants; AT2G42280.1; AT2G42280.1; AT2G42280. [Q66GR3-1]
DR EnsemblPlants; AT2G42280.2; AT2G42280.2; AT2G42280. [Q66GR3-2]
DR GeneID; 818829; -.
DR Gramene; AT2G42280.1; AT2G42280.1; AT2G42280. [Q66GR3-1]
DR Gramene; AT2G42280.2; AT2G42280.2; AT2G42280. [Q66GR3-2]
DR KEGG; ath:AT2G42280; -.
DR Araport; AT2G42280; -.
DR TAIR; locus:2059979; AT2G42280.
DR eggNOG; ENOG502R5PU; Eukaryota.
DR HOGENOM; CLU_042981_0_1_1; -.
DR InParanoid; Q66GR3; -.
DR OMA; DHQIRDK; -.
DR PhylomeDB; Q66GR3; -.
DR PRO; PR:Q66GR3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q66GR3; baseline and differential.
DR Genevisible; Q66GR3; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:TAIR.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd11393; bHLH_AtbHLH_like; 1.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045239; bHLH95_bHLH.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..359
FT /note="Transcription factor bHLH130"
FT /id="PRO_0000358814"
FT DOMAIN 285..335
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 161..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..186
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT VAR_SEQ 297..300
FT /note="RRTR -> TSPS (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036104"
FT VAR_SEQ 301..359
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036105"
SQ SEQUENCE 359 AA; 39908 MW; A41416BEC3D023C8 CRC64;
MDSNNHLYDP NPTGSGLLRF RSAPSSVLAA FVDDDKIGFD SDRLLSRFVT SNGVNGDLGS
PKFEDKSPVS LTNTSVSYAA TLPPPPQLEP SSFLGLPPHY PRQSKGIMNS VGLDQFLGIN
NHHTKPVESN LLRQSSSPAG MFTNLSDQNG YGSMRNLMNY EEDEESPSNS NGLRRHCSLS
SRPPSSLGML SQIPEIAPET NFPYSHWNDP SSFIDNLSSL KREAEDDGKL FLGAQNGESG
NRMQLLSHHL SLPKSSSTAS DMVSVDKYLQ LQDSVPCKIR AKRGCATHPR SIAERVRRTR
ISERMRKLQE LVPNMDKQTN TSDMLDLAVD YIKDLQRQYK ILNDNRANCK CMNKEKKSI
