ID   SIR1_YEAS7              Reviewed;         654 AA.
AC   A7A061;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Regulatory protein SIR1;
DE   AltName: Full=Heterochromatin protein SIR1;
DE   AltName: Full=Silent information regulator 1;
GN   Name=SIR1; ORFNames=SCY_3471;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Involved in the establishment, but not the maintenance, of
CC       heterochromatic silencing at the cryptic mating-type loci HMR and HML.
CC       Is recruited by interacting with the ORC1 subunit of the origin
CC       recognition complex (ORC), which binds to HML-I or HMR-E silencers, DNA
CC       elements that direct the formation of silent chromatin at the mating-
CC       type loci. Establishes transcriptional silencing by recruiting the
CC       three other SIR proteins, SIR2, SIR3, and SIR4, that function directly
CC       in silenced chromatin and establish repression. Also found in
CC       centromeric chromatin. Binds to and helps retain CAC1, a subunit of
CC       chromatin assembly factor I (CAF-I) at centromeric loci independent on
CC       the other SIR proteins (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via OIR domain) with ORC1 (via BAH domain).
CC       Interacts with SIR4. Interacts with CAC1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Note=Associated primarily with the HMR-E silencer at the
CC       HMR locus. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDN60004.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AAFW02000152; EDN60004.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A7A061; -.
DR   SMR; A7A061; -.
DR   TopDownProteomics; A7A061; -.
DR   EnsemblFungi; EDN60004; EDN60004; SCY_3471.
DR   HOGENOM; CLU_449838_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR037240; ORC1-binding_dom.
DR   InterPro; IPR021646; Sir1_ORC-binding.
DR   Pfam; PF11603; Sir1; 2.
DR   SUPFAM; SSF144005; SSF144005; 2.
PE   3: Inferred from homology;
KW   Centromere; Chromosome; Nucleus; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..654
FT                   /note="Regulatory protein SIR1"
FT                   /id="PRO_0000380180"
FT   REGION          322..654
FT                   /note="Sufficient for interaction with SIR4"
FT                   /evidence="ECO:0000250"
FT   REGION          449..587
FT                   /note="ORC interacting region (OIR)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   654 AA;  76810 MW;  50B00D7E37678964 CRC64;
     MLQINSRLAV IDGWLVDTVK RKPINFRSPE VRLLLPNDDD YKKLSQQNLV DWTRLKKDSN
     SVLVGVKSME LFKHIKLVLR EFFLLEDGRI ILKRIRSKLR YKVVKKLTCK CCRLNLPKWG
     TVYIHPMLKD KEKPLAGVCE FSLDVNPDRE YPLIEINVSH QYIIIEGFLL YLNERRLYRW
     NDNNLRSQVG LTKWAHLRKT YNPVSLDILY SLNSNFYFVK DDLLFQLLGK RVFVKFCKVM
     ENGKCGKAPL WYRVKRTTTA KATHIAYAIS NSTAPDSFKS KNNDYRFIVR EKPIVENTIS
     NLDYSDIKKQ QFTEAEVVKR KISADISQIE NVHTQFNSQK EKNNIRVNKV SSEVLDQISK
     FPVSRVTLLL ISAGQDKNYI ELVEELARRL EKICIEKTTQ SLEEIRDTFQ ANPEMQARFD
     KEYYQSIEEY KITLELIKED LLITLIKQME NMWAAEKKFS TEEEYVSPRF LVADGFLIDL
     AEEKPINPKD PRLLTLLKDH QRAMIDQMNL VKWNDFKKYQ DPIPLKAKTL FKFCKQIKKK
     FLRGADFKLH TLPTEANLKY EPERMTVSCS CVPILLDDQT VQYLYDDSII PEFEATSSYA
     TKQSKCGGKM SLQMEPDLLF QEAIRRMRHL TAYDVLRRNY IAAFEELYMG NCND