SIR2C_DICDI
ID SIR2C_DICDI Reviewed; 456 AA.
AC Q54P49;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=NAD-dependent deacetylase sir2C;
DE EC=2.3.1.286;
DE AltName: Full=Silent information regulator sir2C;
GN Name=sir2C; ORFNames=DDB_G0284795;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RX DOI=10.1264/jsme2.23.40;
RA Katayama T., Yasukawa H.;
RT "Developmental and spatial expression of sir2 genes in the cellular slime
RT mold Dictyostelium discoideum.";
RL Microbes Environ. 23:40-43(2008).
CC -!- FUNCTION: NAD-dependent deacetylase, which plays an important role in
CC the regulation of transcriptional repression. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-
CC ADP-D-ribose + L-lysyl-[protein] + nicotinamide;
CC Xref=Rhea:RHEA:43636, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10731,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:17154, ChEBI:CHEBI:29969,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:61930, ChEBI:CHEBI:83767;
CC EC=2.3.1.286; Evidence={ECO:0000255|PROSITE-ProRule:PRU00236};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in growing cells, but at
CC decreased levels in developing cells. Expressed in both prestalk and
CC prespore cells. {ECO:0000269|Ref.2}.
CC -!- SIMILARITY: Belongs to the sirtuin family. {ECO:0000305}.
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DR EMBL; AAFI02000071; EAL65050.1; -; Genomic_DNA.
DR RefSeq; XP_638409.1; XM_633317.1.
DR AlphaFoldDB; Q54P49; -.
DR SMR; Q54P49; -.
DR STRING; 44689.DDB0216433; -.
DR PaxDb; Q54P49; -.
DR EnsemblProtists; EAL65050; EAL65050; DDB_G0284795.
DR GeneID; 8624779; -.
DR KEGG; ddi:DDB_G0284795; -.
DR dictyBase; DDB_G0284795; sir2C.
DR eggNOG; KOG2682; Eukaryota.
DR HOGENOM; CLU_600549_0_0_1; -.
DR InParanoid; Q54P49; -.
DR OMA; HIENCNI; -.
DR PhylomeDB; Q54P49; -.
DR PRO; PR:Q54P49; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IBA:GO_Central.
DR GO; GO:0017136; F:NAD-dependent histone deacetylase activity; IBA:GO_Central.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1600.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR003000; Sirtuin.
DR InterPro; IPR026591; Sirtuin_cat_small_dom_sf.
DR InterPro; IPR026590; Ssirtuin_cat_dom.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF02146; SIR2; 1.
DR SUPFAM; SSF52467; SSF52467; 1.
DR PROSITE; PS50305; SIRTUIN; 1.
PE 2: Evidence at transcript level;
KW Metal-binding; NAD; Reference proteome; Transferase; Zinc.
FT CHAIN 1..456
FT /note="NAD-dependent deacetylase sir2C"
FT /id="PRO_0000393127"
FT DOMAIN 169..445
FT /note="Deacetylase sirtuin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT ACT_SITE 294
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT BINDING 331
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT BINDING 336
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
SQ SEQUENCE 456 AA; 52428 MW; BD793FF9BED69AAB CRC64;
MSKQTQCVHI ENCNINYESI DIQLYDNENI TIPNCYKCSS QDNILICLEC GIVVCMEDCK
LHSNHSIFKI FHGDDKTLWC NDCNELIKES TMIEKCTKNS DLINKKLKDI ENLFKVMKLF
IYYDQRNLKY NKNFDSIDNS IKDIIKNSNI IKNEIKIENE IEIENNKIKE FIKLIKNDKC
KNIIVLTGAG ISVASGIPDF RSVETGLYNN ENVSKFKLPF KEAVFDIDYF KFNPEPFYQL
SKDLYPSGKF KCTPVHYFIK LLSDKGLLLR NYAQNADTLE RIAGIPLDKL IEAHGSFAVS
RCTNCGLEYS QEYIKDSIFN NDPLKSVVPR CKVVQCNNAV IKPDIVFFGE SLPPIFNQNI
LDDINRCDCL IVIGTSLKVQ PIASMVHFFP HFKNIPRLLI NNQIVGENSF GGFNFNNNKN
FDFKMIGDCQ ESVLNLSKLL NWDTELLNLI NSKNHN
