SIR2E_DICDI
ID SIR2E_DICDI Reviewed; 343 AA.
AC Q55DB0;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=NAD-dependent deacetylase sir2E;
DE EC=2.3.1.286;
DE AltName: Full=Silent information regulator 2E;
GN Name=sir2E; ORFNames=DDB_G0270928;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18707607; DOI=10.1111/j.1440-169x.2008.01062.x;
RA Katayama T., Yasukawa H.;
RT "Analysis of Sir2E in the cellular slime mold Dictyostelium discoideum:
RT cellular localization, spatial expression and overexpression.";
RL Dev. Growth Differ. 50:645-652(2008).
CC -!- FUNCTION: NAD-dependent deacetylase, which plays an important role in
CC the regulation of transcriptional repression (By similarity). May play
CC a role in cell cycle. When overexpressed, the cell cycle is
CC accelerated. {ECO:0000250, ECO:0000269|PubMed:18707607}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-
CC ADP-D-ribose + L-lysyl-[protein] + nicotinamide;
CC Xref=Rhea:RHEA:43636, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10731,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:17154, ChEBI:CHEBI:29969,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:61930, ChEBI:CHEBI:83767;
CC EC=2.3.1.286; Evidence={ECO:0000255|PROSITE-ProRule:PRU00236};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18707607}.
CC -!- DEVELOPMENTAL STAGE: Expressed almost at the same level throughout the
CC life cycle. Expressed in PstA, PstB and PstO prestalk cells in the
CC developmental phase and not in the prespore cells.
CC {ECO:0000269|PubMed:18707607}.
CC -!- DISRUPTION PHENOTYPE: Null cells could not be established, suggesting
CC that sir2E might be essential. {ECO:0000269|PubMed:18707607}.
CC -!- SIMILARITY: Belongs to the sirtuin family. {ECO:0000305}.
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DR EMBL; AAFI02000005; EAL72814.2; -; Genomic_DNA.
DR RefSeq; XP_646221.2; XM_641129.2.
DR AlphaFoldDB; Q55DB0; -.
DR SMR; Q55DB0; -.
DR STRING; 44689.DDB0219995; -.
DR PaxDb; Q55DB0; -.
DR EnsemblProtists; EAL72814; EAL72814; DDB_G0270928.
DR GeneID; 8617175; -.
DR KEGG; ddi:DDB_G0270928; -.
DR dictyBase; DDB_G0270928; sir2E.
DR eggNOG; KOG2683; Eukaryota.
DR HOGENOM; CLU_809934_0_0_1; -.
DR InParanoid; Q55DB0; -.
DR OMA; WMNEADL; -.
DR PhylomeDB; Q55DB0; -.
DR Reactome; R-DDI-2151201; Transcriptional activation of mitochondrial biogenesis.
DR PRO; PR:Q55DB0; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0033558; F:protein lysine deacetylase activity; IEA:UniProtKB-EC.
DR GO; GO:0061697; F:protein-glutaryllysine deglutarylase activity; IBA:GO_Central.
DR GO; GO:0036054; F:protein-malonyllysine demalonylase activity; IBA:GO_Central.
DR GO; GO:0036055; F:protein-succinyllysine desuccinylase activity; IBA:GO_Central.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0036047; P:peptidyl-lysine demalonylation; IBA:GO_Central.
DR GO; GO:0036049; P:peptidyl-lysine desuccinylation; IBA:GO_Central.
DR GO; GO:0061698; P:protein deglutarylation; IBA:GO_Central.
DR Gene3D; 3.30.1600.10; -; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR003000; Sirtuin.
DR InterPro; IPR026591; Sirtuin_cat_small_dom_sf.
DR InterPro; IPR026590; Ssirtuin_cat_dom.
DR Pfam; PF02146; SIR2; 1.
DR SUPFAM; SSF52467; SSF52467; 1.
DR PROSITE; PS50305; SIRTUIN; 1.
PE 2: Evidence at transcript level;
KW NAD; Nucleus; Reference proteome; Transferase.
FT CHAIN 1..343
FT /note="NAD-dependent deacetylase sir2E"
FT /id="PRO_0000393270"
FT DOMAIN 35..302
FT /note="Deacetylase sirtuin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
FT ACT_SITE 152
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00236"
SQ SEQUENCE 343 AA; 39579 MW; 802E8C1837E50FF6 CRC64;
MTDDIVFENM VIDDTIVEEE DVEMKYYLKN KKEFEFLAKE MLSGKKILFI TGAGLSINSG
ISAYRNTKTS VWSNFITEWG TRKKFEQDPA QFWNHFWLRT HEKQEYLDAL PNSGHLAISN
FVEYLGSNVI TQNVDALHLK AKVPIEKLVE VHGRISLYKC ITKGCRFEYD DTIDNIEIGD
YSINGTTMKQ GNLEITPPLC PECKKPILPQ SLLFDENYSS HQFYNIEKAM DWIQEADIFI
FIGTSFSVGI TEEVICHAQS ERKKMFNFNI FKETKISGLK SIVGKSEITL PLLERQLLYE
AQKRNNGKKQ IWYGNTIKRI VSENSMLLKE AMLKNDTKSS VKV
