BH148_ARATH
ID BH148_ARATH Reviewed; 221 AA.
AC Q9C8Z9; C0SVA2; Q8L8X9;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcription factor bHLH148 {ECO:0000303|PubMed:24009530};
DE AltName: Full=ATBS1 interacting factor 2 {ECO:0000303|PubMed:20023194};
DE AltName: Full=Basic helix-loop-helix protein 148 {ECO:0000303|PubMed:24009530};
DE Short=AtbHLH148 {ECO:0000303|PubMed:24009530};
DE Short=bHLH 148 {ECO:0000303|PubMed:24009530};
DE AltName: Full=Protein RSA1 INTERACTING TRANSCRIPTION FACTOR 1 {ECO:0000303|PubMed:24009530};
DE Short=AtRITF1 {ECO:0000303|PubMed:24009530};
DE AltName: Full=Transcription factor EN 143 {ECO:0000303|PubMed:14600211};
DE AltName: Full=bHLH transcription factor bHLH148 {ECO:0000303|PubMed:24009530};
GN Name=BHLH148 {ECO:0000303|PubMed:24009530};
GN Synonyms=AIF2 {ECO:0000303|PubMed:20023194},
GN EN143 {ECO:0000303|PubMed:14600211}, RITF1 {ECO:0000303|PubMed:24009530};
GN OrderedLocusNames=At3g06590 {ECO:0000312|Araport:AT3G06590};
GN ORFNames=F5E6.8 {ECO:0000312|EMBL:AAG51338.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP FUNCTION, AND INTERACTION WITH PRE3.
RC STRAIN=cv. Columbia;
RX PubMed=20023194; DOI=10.1105/tpc.109.072504;
RA Wang H., Zhu Y., Fujioka S., Asami T., Li J., Li J.;
RT "Regulation of Arabidopsis brassinosteroid signaling by atypical basic
RT helix-loop-helix proteins.";
RL Plant Cell 21:3781-3791(2009).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH RSA1, SUBCELLULAR
RP LOCATION, AND INDUCTION BY SALT STRESS.
RC STRAIN=cv. Columbia, and cv. Columbia GL1;
RX PubMed=24009530; DOI=10.1371/journal.pgen.1003755;
RA Guan Q., Wu J., Yue X., Zhang Y., Zhu J.;
RT "A nuclear calcium-sensing pathway is critical for gene regulation and salt
RT stress tolerance in Arabidopsis.";
RL PLoS Genet. 9:E1003755-E1003755(2013).
CC -!- FUNCTION: bHLH transcription factor that binds DNA on specific sequence
CC 5'-CANNTG-3' in target gene promoters (PubMed:24009530). Negatively
CC regulates brassinosteroid signaling (PubMed:20023194). Together with
CC BHLH148/RITF1, regulates the transcription of several genes involved in
CC the detoxification of reactive oxygen species (ROS) generated by salt
CC (NaCl) stress. Confers tolerance to salt and to the oxidative stress-
CC inducing reagents hydrogen peroxide H(2)O(2) and methyl viologen (MV)
CC (PubMed:24009530). {ECO:0000269|PubMed:20023194,
CC ECO:0000269|PubMed:24009530}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with PRE3. Binds to RSA1
CC (PubMed:24009530). {ECO:0000269|PubMed:20023194,
CC ECO:0000269|PubMed:24009530, ECO:0000305}.
CC -!- INTERACTION:
CC Q9C8Z9; Q93VJ4: BEE2; NbExp=4; IntAct=EBI-4434374, EBI-4424312;
CC Q9C8Z9; Q8GY61: BHLH63; NbExp=3; IntAct=EBI-4434374, EBI-4469930;
CC Q9C8Z9; Q9LV17: BHLH79; NbExp=5; IntAct=EBI-4434374, EBI-15191993;
CC Q9C8Z9; Q9ZPW3-2: HBI1; NbExp=4; IntAct=EBI-4434374, EBI-15197377;
CC Q9C8Z9; Q9SZI2: NAP1;1; NbExp=3; IntAct=EBI-4434374, EBI-4424361;
CC Q9C8Z9; Q9LXG5: PRE2; NbExp=5; IntAct=EBI-4434374, EBI-4459591;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24009530}.
CC -!- INDUCTION: Slightly induced by salt (NaCl) stress.
CC {ECO:0000269|PubMed:24009530}.
CC -!- DISRUPTION PHENOTYPE: Hypersensitivity to salt (NaCl) stress during
CC seed germination and during seedling growth and development.
CC Hypersensitivity to hydrogen peroxide H(2)O(2) and methyl viologen
CC (MV). {ECO:0000269|PubMed:24009530}.
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DR EMBL; AC020580; AAG51338.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74418.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74419.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63825.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63826.1; -; Genomic_DNA.
DR EMBL; AY081288; AAL91177.1; -; mRNA.
DR EMBL; AY093390; AAM13389.1; -; mRNA.
DR EMBL; AY088744; AAM67062.1; -; mRNA.
DR EMBL; AB493605; BAH30443.1; -; mRNA.
DR RefSeq; NP_001325894.1; NM_001337664.1.
DR RefSeq; NP_001325895.1; NM_001337665.1.
DR RefSeq; NP_566287.1; NM_111535.3.
DR RefSeq; NP_974239.1; NM_202510.1.
DR AlphaFoldDB; Q9C8Z9; -.
DR SMR; Q9C8Z9; -.
DR BioGRID; 5173; 28.
DR IntAct; Q9C8Z9; 22.
DR STRING; 3702.AT3G06590.2; -.
DR PaxDb; Q9C8Z9; -.
DR PRIDE; Q9C8Z9; -.
DR ProteomicsDB; 240468; -.
DR EnsemblPlants; AT3G06590.1; AT3G06590.1; AT3G06590.
DR EnsemblPlants; AT3G06590.2; AT3G06590.2; AT3G06590.
DR EnsemblPlants; AT3G06590.3; AT3G06590.3; AT3G06590.
DR EnsemblPlants; AT3G06590.4; AT3G06590.4; AT3G06590.
DR GeneID; 819838; -.
DR Gramene; AT3G06590.1; AT3G06590.1; AT3G06590.
DR Gramene; AT3G06590.2; AT3G06590.2; AT3G06590.
DR Gramene; AT3G06590.3; AT3G06590.3; AT3G06590.
DR Gramene; AT3G06590.4; AT3G06590.4; AT3G06590.
DR KEGG; ath:AT3G06590; -.
DR Araport; AT3G06590; -.
DR TAIR; locus:2084339; AT3G06590.
DR eggNOG; ENOG502RY2C; Eukaryota.
DR HOGENOM; CLU_090794_0_1_1; -.
DR InParanoid; Q9C8Z9; -.
DR OMA; NNHTRWK; -.
DR OrthoDB; 1412976at2759; -.
DR PhylomeDB; Q9C8Z9; -.
DR PRO; PR:Q9C8Z9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9C8Z9; baseline and differential.
DR Genevisible; Q9C8Z9; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0042542; P:response to hydrogen peroxide; IMP:UniProtKB.
DR GO; GO:0006979; P:response to oxidative stress; IMP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:UniProtKB.
DR CDD; cd11444; bHLH_AtIBH1_like; 1.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR044549; bHLH_AtIBH1-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR044660; IBH1-like.
DR PANTHER; PTHR33124; PTHR33124; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..221
FT /note="Transcription factor bHLH148"
FT /id="PRO_0000358825"
FT DOMAIN 148..197
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 70..84
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 130..132
FT /note="MAI -> VAV (in Ref. 4; AAM67062)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24256 MW; 1592A94433798E55 CRC64;
MASLISDIEP PTSTTSDLVR RKKRSSASSA ASSRSSASSV SGEIHARWRS EKQQRIYSAK
LFQALQQVRL NSSASTSSSP TAQKRGKAVR EAADRALAVS ARGRTLWSRA ILANRIKLKF
RKQRRPRATM AIPAMTTVVS SSSNRSRKRR VSVLRLNKKS IPDVNRKVRV LGRLVPGCGK
QSVPVILEEA TDYIQALEMQ VRAMNSLVQL LSSYGSAPPP I
