SIRA_BACSU
ID SIRA_BACSU Reviewed; 148 AA.
AC P45707;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Sporulation inhibitor of replication protein SirA;
GN Name=sirA; Synonyms=yneE, yoxF; OrderedLocusNames=BSU17900;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9068642; DOI=10.1128/jb.179.6.1962-1973.1997;
RA Schioett T., von Wachenfeldt C., Hederstedt L.;
RT "Identification and characterization of the ccdA gene, required for
RT cytochrome c synthesis in Bacillus subtilis.";
RL J. Bacteriol. 179:1962-1973(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969507; DOI=10.1099/13500872-142-11-3097;
RA Rose M., Entian K.-D.;
RT "New genes in the 170 degrees region of the Bacillus subtilis genome encode
RT DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid
RT transporter.";
RL Microbiology 142:3097-3101(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION AS AN INHIBITOR OF DNA REPLICATION, AND INDUCTION.
RC STRAIN=168 / YB886 / BG214;
RX PubMed=19329632; DOI=10.1128/jb.00216-09;
RA Rahn-Lee L., Gorbatyuk B., Skovgaard O., Losick R.;
RT "The conserved sporulation protein yneE inhibits DNA replication in
RT Bacillus subtilis.";
RL J. Bacteriol. 191:3736-3739(2009).
CC -!- FUNCTION: Inhibits DNA replication in growing cells, thus reducing
CC chromosome copy number and playing a significant role during the onset
CC of sporulation. {ECO:0000269|PubMed:19329632}.
CC -!- INDUCTION: Up-regulated by spo0A. {ECO:0000269|PubMed:19329632}.
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DR EMBL; X87845; CAA61114.1; -; Genomic_DNA.
DR EMBL; Z73234; CAA97617.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13674.1; -; Genomic_DNA.
DR PIR; S57402; S57402.
DR RefSeq; NP_389673.1; NC_000964.3.
DR RefSeq; WP_003231591.1; NZ_JNCM01000035.1.
DR PDB; 4TPS; X-ray; 1.65 A; A/C=1-148.
DR PDBsum; 4TPS; -.
DR AlphaFoldDB; P45707; -.
DR SMR; P45707; -.
DR IntAct; P45707; 1.
DR STRING; 224308.BSU17900; -.
DR PaxDb; P45707; -.
DR PRIDE; P45707; -.
DR EnsemblBacteria; CAB13674; CAB13674; BSU_17900.
DR GeneID; 936340; -.
DR KEGG; bsu:BSU17900; -.
DR PATRIC; fig|224308.179.peg.1951; -.
DR eggNOG; ENOG5033MW4; Bacteria.
DR OMA; KDEFANH; -.
DR BioCyc; BSUB:BSU17900-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.30.310.250; -; 1.
DR InterPro; IPR019683; SirA.
DR InterPro; IPR038449; SirA_sf.
DR Pfam; PF10747; SirA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication inhibitor; Reference proteome; Sporulation.
FT CHAIN 1..148
FT /note="Sporulation inhibitor of replication protein SirA"
FT /id="PRO_0000049647"
FT STRAND 3..10
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 12..17
FT /evidence="ECO:0007829|PDB:4TPS"
FT TURN 18..20
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 22..33
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 39..52
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 58..69
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 80..84
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 86..88
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 90..97
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 100..108
FT /evidence="ECO:0007829|PDB:4TPS"
FT HELIX 110..122
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 126..130
FT /evidence="ECO:0007829|PDB:4TPS"
FT TURN 131..134
FT /evidence="ECO:0007829|PDB:4TPS"
FT STRAND 135..138
FT /evidence="ECO:0007829|PDB:4TPS"
SQ SEQUENCE 148 AA; 18142 MW; 1EBB6477E8D6942D CRC64;
MERHYYTYLI KEEFANHYFG RESVMFELFQ DYHWTSLEKQ QYEMTEKQIQ YITQPIPILH
MHQRLKMNLN KTDYRQLDYI YRIALPKAKG HATFMMKEHM IEIVASGDYE AETIFFEVLR
KVSPCFLAMD FNSKRYGWLN PVKERNFV
