SIRBL_HUMAN
ID SIRBL_HUMAN Reviewed; 398 AA.
AC Q5TFQ8; Q7Z3B9;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Signal-regulatory protein beta-1 isoform 3;
DE Short=SIRP-beta-1 isoform 3;
DE Flags: Precursor;
GN Name=SIRPB1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-23.
RC TISSUE=Caudate nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-23.
RC TISSUE=Esophageal carcinoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 30-148, AND DISULFIDE BOND.
RX PubMed=18657508; DOI=10.1016/j.molcel.2008.05.026;
RA Hatherley D., Graham S.C., Turner J., Harlos K., Stuart D.I., Barclay A.N.;
RT "Paired receptor specificity explained by structures of signal regulatory
RT proteins alone and complexed with CD47.";
RL Mol. Cell 31:266-277(2008).
CC -!- FUNCTION: Immunoglobulin-like cell surface receptor involved in the
CC negative regulation of receptor tyrosine kinase-coupled signaling
CC processes. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=3;
CC IsoId=Q5TFQ8-1; Sequence=Displayed;
CC Name=1;
CC IsoId=O00241-1; Sequence=External;
CC Name=2;
CC IsoId=O00241-2; Sequence=External;
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DR EMBL; AK289866; BAF82555.1; -; mRNA.
DR EMBL; BX537992; CAD97951.1; -; mRNA.
DR EMBL; AL049634; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL138804; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL592544; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS46571.1; -. [Q5TFQ8-1]
DR RefSeq; NP_001129316.1; NM_001135844.3. [Q5TFQ8-1]
DR RefSeq; NP_001316086.1; NM_001329157.1. [Q5TFQ8-1]
DR PDB; 2JJV; X-ray; 1.80 A; A/B=30-148.
DR PDBsum; 2JJV; -.
DR AlphaFoldDB; Q5TFQ8; -.
DR SMR; Q5TFQ8; -.
DR BioGRID; 115609; 10.
DR GlyConnect; 1747; 11 N-Linked glycans (2 sites).
DR iPTMnet; Q5TFQ8; -.
DR BioMuta; SIRPB1; -.
DR DMDM; 205829937; -.
DR jPOST; Q5TFQ8; -.
DR MassIVE; Q5TFQ8; -.
DR MaxQB; Q5TFQ8; -.
DR PaxDb; Q5TFQ8; -.
DR PeptideAtlas; Q5TFQ8; -.
DR PRIDE; Q5TFQ8; -.
DR ProteomicsDB; 65089; -. [Q5TFQ8-1]
DR Antibodypedia; 23124; 348 antibodies from 34 providers.
DR DNASU; 10326; -.
DR Ensembl; ENST00000279477.11; ENSP00000279477.7; ENSG00000101307.16. [Q5TFQ8-1]
DR Ensembl; ENST00000568365.1; ENSP00000456826.1; ENSG00000101307.16. [Q5TFQ8-1]
DR GeneID; 10326; -.
DR KEGG; hsa:10326; -.
DR UCSC; uc002wfl.5; human. [Q5TFQ8-1]
DR CTD; 10326; -.
DR DisGeNET; 10326; -.
DR GeneCards; SIRPB1; -.
DR HGNC; HGNC:15928; SIRPB1.
DR HPA; ENSG00000101307; Tissue enhanced (bone marrow, lung, lymphoid tissue).
DR neXtProt; NX_Q5TFQ8; -.
DR OpenTargets; ENSG00000101307; -.
DR PharmGKB; PA38051; -.
DR VEuPathDB; HostDB:ENSG00000101307; -.
DR GeneTree; ENSGT00960000186656; -.
DR HOGENOM; CLU_044430_0_0_1; -.
DR OMA; DNTETHN; -.
DR PathwayCommons; Q5TFQ8; -.
DR BioGRID-ORCS; 10326; 10 hits in 1065 CRISPR screens.
DR ChiTaRS; SIRPB1; human.
DR EvolutionaryTrace; Q5TFQ8; -.
DR GenomeRNAi; 10326; -.
DR Pharos; Q5TFQ8; Tbio.
DR Proteomes; UP000005640; Chromosome 20.
DR Bgee; ENSG00000101307; Expressed in monocyte and 121 other tissues.
DR ExpressionAtlas; Q5TFQ8; baseline and differential.
DR Genevisible; Q5TFQ8; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003597; Ig_C1-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07654; C1-set; 2.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 2.
DR SMART; SM00407; IGc1; 2.
DR SMART; SM00406; IGv; 1.
DR SUPFAM; SSF48726; SSF48726; 3.
DR PROSITE; PS50835; IG_LIKE; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..398
FT /note="Signal-regulatory protein beta-1 isoform 3"
FT /id="PRO_0000349108"
FT TOPO_DOM 30..371
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 31..136
FT /note="Ig-like V-type"
FT DOMAIN 147..246
FT /note="Ig-like C1-type 1"
FT DOMAIN 253..347
FT /note="Ig-like C1-type 2"
FT REGION 337..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 244
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 54..120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114,
FT ECO:0000269|PubMed:18657508"
FT DISULFID 169..227
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 272..330
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VARIANT 23
FT /note="R -> G (in dbSNP:rs1535882)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:17974005"
FT /id="VAR_056077"
FT VARIANT 51
FT /note="T -> I (in dbSNP:rs1135196)"
FT /id="VAR_059411"
FT STRAND 40..45
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 76..80
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 95..97
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:2JJV"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 116..124
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 131..135
FT /evidence="ECO:0007829|PDB:2JJV"
FT STRAND 139..144
FT /evidence="ECO:0007829|PDB:2JJV"
SQ SEQUENCE 398 AA; 43359 MW; 7F3B930235BD556C CRC64;
MPVPASWPHL PSPFLLMTLL LGRLTGVAGE EELQVIQPDK SISVAAGESA TLHCTVTSLI
PVGPIQWFRG AGPGRELIYN QKEGHFPRVT TVSDLTKRNN MDFSIRISNI TPADAGTYYC
VKFRKGSPDH VEFKSGAGTE LSVRAKPSAP VVSGPAARAT PQHTVSFTCE SHGFSPRDIT
LKWFKNGNEL SDFQTNVDPA GDSVSYSIHS TAKVVLTRED VHSQVICEVA HVTLQGDPLR
GTANLSETIR VPPTLEVTQQ PVRAENQVNV TCQVRKFYPQ RLQLTWLENG NVSRTETAST
LTENKDGTYN WMSWLLVNVS AHRDDVKLTC QVEHDGQPAV SKSHDLKVSA HPKEQGSNTA
PGPALASAAP LLIAFLLGPK VLLVVGVSVI YVYWKQKA
